LOCUS       sequence-404840-        2884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT5G60160
FEATURES             Location/Qualifiers
     source          1..2884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      290..878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     779..798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1032..1049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1800..1818)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1886..1990
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2212..2884,1..119)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
       61 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
      121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
      181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
      241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat cactctgtgg tctcaggagg ccttttttaa
     1141 ccctcttaat attatttata tatatgattt tgtctgccag atccacgttg ctgtcttgtt
     1201 aaaaaaaaaa aaaaaagggt ttctagaatt tattaccctt cgatttctaa ttttattacc
     1261 acttagtccg gttcggtttt gattggttta atggtgtttg aatgtgttac taccgaaatt
     1321 acccaaccac ataaaatctt ctagttttgg gaattcttat gttattacca aaattatcca
     1381 accacatata aggaagggta tttatagtat atgactacaa aatttatttg aaagttttta
     1441 aaatgaacaa atttttaaat agtgcgttaa ttcaaaattt caaatgaaac aaatttttgt
     1501 ataataattt attgaaagtt tttacataaa agtataaaac gtttatatga ataaataaca
     1561 ttttttttat gtactcatga ataaataact atttatttaa aaaaccaatt gggctttgta
     1621 atgggccaat aaaacttctc gggttactaa aatggaaacg gaacctccgg caagacttac
     1681 acagggacga tcggaaaccg aacatccatt ggagtgctgc ctcacaaaaa cccgaagaac
     1741 tcaagttaag agatccagcc aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg
     1801 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1861 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1981 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     2041 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
     2101 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     2161 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     2221 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     2281 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     2341 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     2401 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     2461 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     2521 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     2581 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     2641 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     2701 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     2761 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     2821 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     2881 atac
//
