LOCUS       sequence-404845-        3213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT2G43070
FEATURES             Location/Qualifiers
     source          1..3213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      276..864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     765..784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1018..1035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2115..2133)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2201..2305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2527..3213,1..105)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
       61 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
      121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1081 tggccgattc attaatcact ctgtggtctc agctttaaca tgattcaaca atattctaat
     1141 gatgtgttat caatcaaact gaattagctt ttccccaaaa catcgagcat agcttaagca
     1201 gatttcatat gtgagaggta cacaaaagct gatgatgtaa gttctctgca aagccaaagt
     1261 taatcggata ccaaaagtaa ttcaagacac atcggaaagt actgtattat atcgtttttt
     1321 tttccttgtt ttgggggaga agaaagaaag gttctctagt aagactcatg acatgttgta
     1381 cagagtctat attaaccttc tcttgaattc agactcgttt ttgtagaatt tcaagtataa
     1441 tgcaaattcc cttttgttaa tctttgtgtg ttttcttttt tgggaaaaat aagtctttga
     1501 aaattccata aaacttgaaa gtttgttcaa atattattcg actgatggaa tgaaattctg
     1561 acgaaaacga atgcacgaat cagatatttg atgtgaaata tgtggaattg accaatttgt
     1621 ctgtttacgt acacgtgtat aataattgac aaattaaagt aggacactcg tatgaaatat
     1681 aagtatgacc aactgcataa ataagacgtg tagataatcc ttccaacgga aaatgtgatt
     1741 ataaagaaaa caagtaatca gagtcttgta atttttgtta ataatgttgt tgtgagcgac
     1801 catttgaatt caatccgaca taaatcatga aatgttagag ttatacaaaa aaaaaaatct
     1861 ctcttacctg aaaaaagagt tatacaaaac aaaaaaggag ttaaattata tatattctac
     1921 aaaattcttt aacctatata atgtcaagta tttttaagac ttattcaagt aactccgtgt
     1981 taaacaaaaa aaaatcattt aattaaatga ttcataattc tataaatata tatttttaaa
     2041 attattagat ttatcatata ttttctcaaa aacatcgctt gagaccacga agtggctctt
     2101 cagtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2161 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2221 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2281 tcaataatat tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2341 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gttttttttt
     2401 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     2461 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     2521 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     2581 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     2641 gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt
     2701 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     2761 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     2821 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     2881 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     2941 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     3001 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     3061 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     3121 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     3181 cagtatcagc ccgtcatact tgaagctaga cag
//
