LOCUS       sequence-404849-        2949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT5G37830
FEATURES             Location/Qualifiers
     source          1..2949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      276..864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     765..784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1018..1035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1851..1869)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1937..2041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2263..2949,1..105)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
       61 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
      121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
      181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
      241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1081 tggccgattc attaatcact ctgtggtctc agcttacaat tcatctcctc ctcataagtc
     1141 tcttctcgaa cttcattact ccttcattta acgtataatc caaattagtt gtttatgtgt
     1201 cttgttgttg tttcggtgtc tcttagccgg atattctttg gaggtttggc cattttcagt
     1261 gaaataagga tcagattctt gattcattag aaacaaggta ataaattgtc ttggtgaata
     1321 agttttagtt gttgttgtat gtacttatgt atcactattg ctggtccaag acttttggct
     1381 ggatttcatt gatgccattg tcgacattga aaagagattt gtgtctgcta tgatcggtcc
     1441 aacaaaacta gtcgtctgaa gcaaccaaaa aaaaaagtgg ccccttgtat aatttgtcgt
     1501 agtaatgtat tttttcctat tttgtgtaaa tttgaattct aattaatttt taaaaggaat
     1561 acaaaagaaa ataaaaggca aaaaaaggga tagaaagatg agaagttagg taagatacaa
     1621 ttaaatgaag gattttgctt tttttttgtc caccatatta aagaaggaaa attagaaata
     1681 tgtggccttc acaaactatt ttctaaaata aacgatattg ttttaaatga aaccatgggc
     1741 ttatgaatat tgctccctaa aaggctaaaa tccattatat ttatcaaaaa atatgacctt
     1801 caataaactc acgcttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     1861 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2041 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     2101 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     2161 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2221 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     2281 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2341 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     2401 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2461 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2521 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2581 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2641 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2701 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2761 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2821 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2881 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2941 gctagacag
//
