LOCUS       sequence-404850-        3614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo compatible L0 plasmid containing the CrGLK coding sequence, 
            lacking a stop codon to allow for C-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pLC045 - pL0_CrGLK (CDS1ns)
FEATURES             Location/Qualifiers
     source          1..3614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1154..1172)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1240..1344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1566..2357
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3017..3036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3270..3287
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgtttctct tgatcatcaa catcatgaac attctcaaat tacctcctca atttcggcgg
       61 ctgattatga taaactagta gaattagagg ataaggctaa ttcatcatcc gcttcagacg
      121 acgtcggttc aggctcatct actttaactc aaggagaaga tgttgttagc aaatcaacgg
      181 ttttaaatcc atcttctaaa gaaggtgata aaggaaggaa gtcatcttct actcaatcaa
      241 agaattcttc tgggaagaag aaagtgaagg tggattggac accagagctg cacagaagat
      301 ttgtacaagc agtggagcag ctaggtgtgg ataaggcagt cccttcaaga attcttgaac
      361 ttatgggaat tgattgtctt actcgccaca acattgctag ccatcttcaa aaatacagat
      421 cccacaggaa acatttgcta gcaagagaag ctgaggcagc aaattggagc cagaggcggc
      481 aattatacgg aggcactgca gcagcagcag gaggagggaa gagagatgta aatccatggc
      541 ttgctccaac catgggattt cctcctccca tggcaccggc ggccgtccct cattttagac
      601 ccttacatgt atggggtcac ccatctgtcg accaatcttt aatgcacgtt tggcctaaac
      661 atttagctcc tccgccccga ccaccaacat ggccaccaac tccaacacca acaccaacac
      721 caccaccacc accgccagat cctaattcct tttggcattc acctcaccaa cgtgtaccaa
      781 accctcttac cccaggaacg ccttgctttc cgccaccttt aacaccagcg agatttccaa
      841 caccgccggt ccccggaata ccaccacctc caatgtacaa agtagacccg gtcgcaggca
      901 tgggtgtgcc ggcacctgct accggacaag cagtacccca tgcacctcct cttgactttc
      961 acccctcgac ggagagcgta gatgcagcta tcggagatgt tttatcgaaa ccctggctgc
     1021 cacttcctct tgggctgaaa ccgccgtcga ttgacagtgt actggtggag ctacagcggc
     1081 aaggaatacc gaaaattcca ccagtttgta cgggttcgtg agaccacgaa gtggctcttc
     1141 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1201 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1261 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1321 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     1381 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
     1441 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1501 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1561 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1621 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1681 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     1741 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1801 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1861 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1921 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1981 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2041 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2101 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2161 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2221 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2281 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     2341 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     2401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc
     3361 tcaaatgctt gctgtgtcac cattgaggag caataataat gagagaaaaa aggagatatg
     3421 tgaaattagt agtagtacta gttttccagt tgttgatgat tttcctgatt ttgggggagc
     3481 aaatttgctt gatagcattg attttgatga actttttgtt gggattaacg gtgaggatga
     3541 tgttttacca gatttggaaa tggatcctca tgatattctt gctgaattct cacttactga
     3601 atcattatca ccat
//
