LOCUS       sequence-404851-        2590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G64550(E,A)
FEATURES             Location/Qualifiers
     source          1..2590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(381..399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        467..571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             793..1584
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1755..2343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2244..2263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2497..2514
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggagttggcg aaacaattaa gaggtctagt tttggtaaaa tccaaattgc taatctatca
       61 tctctcaaac atgtgttaac aaaaacaaaa ttgaggatct caagctagac tctagtgagg
      121 agccatgtta agagggaaca acgttaaccg gattaaatca taatatttgg tttggtttag
      181 taaattagca aaggtgatta actgcaaagg tgcctgaggg agaaggttcc aaatagccct
      241 aaaaagcttc gccttctctc cttgccttca tttcgggtgt ggaggaaagt tatcaaatca
      301 gtttttgtgg aattttgaat ctcgtcgctt ctgtgttggc taatgtgaga ccacgaagtg
      361 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      421 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      541 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
      601 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      661 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
      721 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
      781 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
      841 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
      901 gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta
      961 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     1021 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     1081 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     1141 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     1201 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     1261 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     1321 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     1381 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     1441 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     1501 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
     1561 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
     1621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1861 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc
     2581 tgtggtctca
//
