LOCUS       sequence-404853-        3063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT3G07160
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      289..877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     778..797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1031..1048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1978..1996)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2064..2168
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2390..3063,1..118)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
       61 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact
      121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
      181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
      241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      421 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      901 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      961 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1021 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     1081 tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagac gttgcgagag
     1141 agtattcggg aaattaacat ccaatcaaag taacgggtcg ggtcgggtcg agaatcagga
     1201 ttatgcgttc agaacaaaaa aaacgataac cggtttgact gttaaccaga gttaatccgg
     1261 cgattcaaaa gacgtttcgg ttcgattttt ctttgacaac gcggctaagc ttttcatttc
     1321 tttttttttg ttttgtcaac acgcggttaa atttttgttt gtcagattca ttttttttct
     1381 tgataaaaaa catccagatt cctttctttg taatgtttta gattaaaata aacaaaaaat
     1441 ccctaaaaaa cgaaaaagtt ttgaaactta gaaagacgga cataagcgag ctagcttcta
     1501 agctttcttc gctaaattct tggctaattc tcttctatga ctgatccaat tctgcttcga
     1561 ctccatatct gaggtttctg acgtctgatt aggtaactcc cgcttttgtt ttttttgggg
     1621 ggttttatcc atttttaatg tctttgttgt ctacttgttt caatcgtatg aacttgagac
     1681 gcgagaagct cagattcagg atccctaaat ttttctctat tttttttttt agttttgttt
     1741 cgttgattac aactagtcct aggttaaaga acaacgttta caacttattt tgcttttttc
     1801 agttgtgcct aggtcacttg tccgaatctg tataattcac gtgtatcttc ctggatttga
     1861 ttacatttca tttacttagt gttgggtttt acgttagttt aagctgctga gtactttgat
     1921 gttttttgtt tgtagaaaaa tgtgagacca cgaagtggct cttcagtgga cgaaagggcc
     1981 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2041 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2101 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2161 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2221 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     2281 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2341 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     2401 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2461 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2521 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2581 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2641 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2701 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2761 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2821 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2881 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2941 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3001 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3061 act
//
