LOCUS       sequence-404856-        2256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo compatible L0 plasmid containing a stop codon. To allow for 
            proper fusion with CDS1ns L0 plasmids, this plasmid adds an 
            additional glycine and serine to the C-terminal end of a CDS and 
            then adds a stop codon to terminate translation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, 
            Lee-Parsons CWT
  TITLE     The role of the Golden2-like (GLK) transcription factor in 
            regulating terpenoid indole alkaloid biosynthesis in Catharanthus 
            roseus.
  JOURNAL   Plant Cell Rep. 2024 May 14;43(6):141. doi: 
            10.1007/s00299-024-03208-9.
  PUBMED    38743349
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2024-05-14"; volume: "43(6):141. doi"; pages: " 
            10.1007/s00299-024-03208-9"
COMMENT     Sequence Label: pLC025 - pL0_StopCodon (CT)
FEATURES             Location/Qualifiers
     source          1..2256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     455..472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(595..613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        681..785
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1007..1798
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1969..2256,1..301)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
       61 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      361 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      421 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      481 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg
      541 tggtctcatt cgtaagcttt gagaccacga agtggctctt cagtggacga aagggcctcg
      601 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      781 agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      841 cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg
      901 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
      961 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
     1021 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1081 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     1141 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     1201 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     1261 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     1321 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     1381 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     1441 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     1501 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     1561 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     1621 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     1681 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     1741 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact
     1801 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1861 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1921 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1981 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2041 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2101 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2161 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2221 taagtcgtgt cttaccgggt tggactcaag acgata
//
