LOCUS       sequence-404859-        2590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G64550
FEATURES             Location/Qualifiers
     source          1..2590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      274..862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     763..782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1016..1033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1490..1508)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1576..1680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1902..2590,1..103)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
       61 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
      121 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
      181 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
      241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      541 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      601 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      661 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      721 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      781 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      841 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      901 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      961 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     1021 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     1081 gccgattcat taatcactct gtggtctcag gagttggcga aacaattaag aggtctagtt
     1141 ttggtaaaat ccaaattgct aatctatcat ctctcaaaca tgtgttaaca aaaacaaaat
     1201 tgaggacttt tgatttcaca atggccttga gccatgttaa gagggaacaa cgttaaccgg
     1261 attaaatcat aatatttggt ttggtttagt aaattagaaa acgtactgta tactctctct
     1321 gcctgaggga gaaggttcca aatagcccta aaaagcttcg ccttctctcc ttgccttcat
     1381 ttcgggtgtg gaggaaagtt atcaaatcag tttttgtgga attttgaatc tcgtcgcttc
     1441 tgtgttggct aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata
     1501 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1681 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1741 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     1801 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1861 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     1921 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     1981 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2041 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2101 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2161 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2221 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2281 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2341 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2401 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2461 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2521 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2581 ctagacaggc
//
