LOCUS       sequence-404866-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 3 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB3-A-TelR
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             199..221
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    469..490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(505..510,511..528,529..535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    543..559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             577..588
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             595..1272
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1317..1388
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1404..1431
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(5397..5414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5568..6156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5648..5667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6327..7118,7119..7187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6950..6969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7188..7292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7360..7378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(7382..7425)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    7428..7447
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      7527..7558
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcttcgccac gatctggctg attttgtcgg ccagcgcggc aactgcgccg tgctccatca
       61 gattcgacag ctcgttgagg gaatccgccc cgtcgatgcc ggccccgtac tcgccaatcg
      121 tcgccggcga cgcgaagagg gcgggcaaaa cctccccctt caatcgcgcc atgttcacgc
      181 ttgttcttcc atggtatatc tccttcttaa agttaaacaa aattattcgg aacccagcat
      241 gatattccgg aaataccaac taagtcaacg gctgatatcc attgctgttg acaaagggaa
      301 tcaggggatc ttgaagttcc tattccgaag ttcctattct ctagaacgtc tcaatctcta
      361 tagagaccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      421 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      481 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      541 aattgtgagc ggataacaat ttcacacata ctagagaaag aggagaaata ctagatggct
      601 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
      661 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
      721 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
      781 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
      841 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
      901 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
      961 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     1021 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     1081 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     1141 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     1201 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     1261 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
     1321 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1381 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttataggt
     1441 ctcaggactt agagagacgc tgcagttgca agacaatatc gaccgcttgc agcttgataa
     1501 agtgatcaaa gagggacgag cagctacagc actgctaagg gagatcggag agccactgcc
     1561 gccaccagtg aaagtgaaaa caggttggtt tagtgctgat tattacggct ttgaggacat
     1621 gctcacacgt tgggataatg atcaatctag cctttccatt tatcacagag agataaaaga
     1681 ggagcaacag gaacagcaac gaaaacaaca gcagatagaa agagaaacta aacaaaaaca
     1741 ggaagatgac aaaaagaaac ggcaggacta tgaaagatat ttatcaaaat acggctatgt
     1801 caaagatgct gagtatgacc atatcagcga ccatgttgct tcagacattt ccatgtttac
     1861 acgtcttcag gcgcaggcat gggcaagtaa cgatatgcaa gagtacataa ggtgttcaaa
     1921 gcaaaaatac gagcagatca gcgacagaat agcctatgag cgtgacttga aaaaaatcgg
     1981 gcaattgagc cgtatcctgg ataaagacag acaggcactt gctgatattt taccgcagtt
     2041 gatgccttac tatgagcaga tgcaacaggc tgttaataaa cggaaaattt tacttgagaa
     2101 tgagcgacag ccatcattta aaccacgtta tgaccaagaa cagcctaagc cgaaaaaaga
     2161 caatgaccta actttttaat gtgttacaat agcattcaaa tgtaacacat tggagtgaaa
     2221 aacatgacag aagcgacttt tacctttcgg gtagaccatg acctaaagca agaattttca
     2281 agccttgcta agactgttga tcggtcgggg gcgcagctaa tccgtgactt catgcgtgac
     2341 tttgttaaga agcaacagga agccgcagac tatgacaaat ggttcaagca gcaggtacag
     2401 atcggcttaa atgaagccaa tgcaggcaag ttgattccac atgaggacgt aaaggcagag
     2461 tttgccgcta gacgtgctgc aacattggcg aaactggcag cgaagaatga agattgagtg
     2521 gactgaaaca gctcgccaag atcgtagaaa tatctatgat tatcttgaag aacgcaaccc
     2581 tatagcagct attgaaattg atgatttaat tgaagaaaag acagatttac ttgttgataa
     2641 tcgactgatg gggcgcacag gcagacagaa agatactagg gagttagtga tacatccgca
     2701 ttatgtggtt gtatatgaca tcactgatat aatacggata ctcagagtgc tacacacatc
     2761 gcaggagtgg tcatgactta ctcatgtact ttggattatt tagtgttata aaatcctgat
     2821 ttataaattt tttttgttaa aaaagataaa agccccttgc aattgcttgg ggctttaccg
     2881 taatttatgg ggtacagatc ttcgatactg acatatcggc aatcgaaagc attaaggttt
     2941 gacgaccgct aatgatttca ccacaggggc ttaatgtacc tgtcttaaat tctaaggttt
     3001 taactcgctt tgtcaagcat agaccccaaa aatttagcca atgtctgtaa ctcaatctgt
     3061 ccatgtgtgg gtgatgaggt acagtgacgc tagcacacat cggaaaaacg ctattactag
     3121 gggaactgaa cagagtagcg gacgcaatga gtagtcattt aattggcggt tatgagcgtg
     3181 ttcaggcggt gctatcaatc gtaatcataa cagtggcagc ttgatacagt gatgtcatcc
     3241 ctgatgcgaa agcgaccgac cgacggtaca tcgaatggga atactttagg gtgattttta
     3301 agaatcgctc tagggtgagt atttcccatt cagctctgct ccctccctct ggtactttaa
     3361 tcaaaagcac tactaaacat atgtttttaa ataaaaaata ttgatataga gataatatta
     3421 gtaagaataa ttaaacaatt gaatatagat aaatcattgt taaataaaga ttaattatta
     3481 aaatgaatgt atacttatat ataaatcaat gatttaaaat atttgataaa gaaaactttt
     3541 caaaaaaaat ataattgaga ttgtgtcatt tcggtcaatt cttaatatgt tccacgcaag
     3601 ttttagctat ggtgctaaac agaaatttgc tgaaaaagaa cttttcactg aactggttaa
     3661 aatgtaagca gcctgagagc cgccaaaaat tttaaaaaca aaccgcctta atcatcttca
     3721 aaaaatacct ctaaaacctc accatttgcg ttttaagacc catatttcat cctgccctta
     3781 tgttcccatg ctgatagcta taaagtgtct gtaatcgctt cctatgacgt tctaggctgt
     3841 tgataacttt tggaacaacg caaaatgtta aaatccgatc attttttaac ctagttattt
     3901 tcgttacagg gtaactttgg tagtattatt tcaatattta gttgttacag gataacaaaa
     3961 tatatgttac agggtaatta atttagcgtt acaggataac tatagttaca gggtaacgtt
     4021 aaatagttat cctgtaattt aaaaatagtt gcaggatagc ataaattttt acaaataaat
     4081 tttataaaaa gttatcctgt aactaaaaac aatgttatcc tgtaactatg atatactata
     4141 tgggagttaa agacatgaaa gacccgaacg atcagaaaac acaggacatg ctaaaagagc
     4201 cgccaaaaac caatgccgaa cgacaaaaag catatcgtga aaaacgcaaa agccttgata
     4261 gtcaacgttt ggaggtgttt atagataagg gtgtgtcgga tatgcttgcg gacatggtgg
     4321 gcgcagcagg ggagagccaa aaagccattt tgaccgcatt gattgagaaa gagtataagc
     4381 ggctgtatgc agtgaaaaat agcaagctaa aaaagtacat tgattcaaaa agtaatgcag
     4441 ataaaaaaga aaccctcgat ttatgagggt tttttataac tactaatgcc tatgaaaata
     4501 agtgcttatc attttctcta atgtacccaa taaaatcggg gttttgttcc acaagtttct
     4561 tgatgtcatt atcatgtttc ttcagtaagt aagccccgat caggatcttg cgttttgcat
     4621 cgtctgacct tgcctgtttt tgctctcgac ctttcaccac agcgtcagct agatcctttc
     4681 ttgccttttc ctgacgttcc aatttttcct tttcttcatc gagcatttga cccaattcca
     4741 tagctgtttt tttgaccatt tttaatgcct gtgtctcaac ctattttccg aagtatgaac
     4801 catcatcaat gtgaaaagtg ttctacttca aaaactcgac ttacatcaag atccgagcgc
     4861 agcgagcaaa atcaaaaaac aaagtcaaag ccttattgct cttgcttttg cttttagcct
     4921 cgcagagttc ccgaagggcg cacttacgca aaatttttgc tacgccaaat tttgcaagta
     4981 cggtcaggga aaccccgaca ccccaaccgc ccaaaacttg gggcggctgt taataaacag
     5041 gtgatgaaaa tggcaattta ccattgtgaa atgcagaaca tttcgaggtc agatggtcgc
     5101 tcaatcgtgg catgtgcagc ataccgagca ggcgaaaaat tgtactgtga tacgtacgga
     5161 aaagagcagg actacaccaa aaaaacaggc attgaataca cccaaatttt tgccccaact
     5221 ggggcaagtc ctgacatgtt agatcgtcaa accctatgga atcgggtaga gcaatccgaa
     5281 ctaaaaaaga acggtgacat caaacaggag gcaagattag caaaggaagt agagatagca
     5341 ttgccgcatg aactggataa gacacagcgt caggcaactt gttaactgca ggcttcctcg
     5401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
     5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6121 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6301 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6361 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6421 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca
     6481 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6541 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6601 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6661 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6721 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6781 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6841 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6901 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6961 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7021 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7081 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7141 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7261 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7321 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     7381 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
     7441 cttctagaaa gtataggaac ttcagagcgc ttttgaagct gatgtgctta aaaacttact
     7501 caatggaatt agctcctcga gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg
     7561 agggggatcg atcccttatg gctctgcacc cggctccatc accaacaggt cgcgcacgcg
     7621 cttcactcgg ttgcggatcg acactgccag cccaacaaag ccggttgccg ccgccgccag
     7681 gatcgcgccg atgatgccgg ccacaccggc catcgcccac caggtcgccg ccttccggtt
     7741 ccattcctgc tggtactgct tcgcaatgct ggacctcggc tcaccatagg ctgaccgctc
     7801 gatggcgtat gccgcttctc cccttggcgt aaaacccagc gccgcaggcg gcattgccat
     7861 gctgcccgcc gctttcccga ccacgacgcg cgcaccaggc ttgcggtcca gaccttcggc
     7921 cacggcgagc tgcgcaagga cataatcagc cgccgacttg gctccacgcg cctcgatcag
     7981 ctcttgcact cgcgcgaaat ccttggcctc cacggccgcc atgaatcgcg cacgcggcga
     8041 aggctccgca gggccggcgt cgtgatcgcc gccgagaatg cccttcacca agttcgacga
     8101 cacgaaaatc atgctgacgg ctatcaccat catgcagacg gatcgcacga acccgcagaa
     8161 ctcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa
     8221 aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga agggcaggcc
     8281 gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac
     8341 ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc
     8401 cccgatcccg gcaatggcaa ggactgccag cgccgcgatg aggaagcggg tgccccgctt
     8461 cttcatcttc gcgcctcggg cctccaggcc gcctacctgg gcgaaaacat cggtgtttgt
     8521 ggcattcata cggactcctg ttgggccagc tcgcgcacgg gctggcgggt cagcttggct
     8581 tgaagatcgc cacgcattgc ggcgatctgc ttctcggcat ccttgcgctt ctgcacgcct
     8641 tcctgctgga tgcgaataac gtcctcgacg gtcttgatga gcgtcgtctg aacctgcttg
     8701 agcgtgtcca cgtcgatcac caggcgttgg ttctccttcg ccgtttcgac ggacgtgcga
     8761 tgcagcaggg ccgcattgcg cttcatcagg tcgttggtgg tgtcgtcgat ggccgtggcc
     8821 agttcgacgg cgttcttctg ctcgttgagg ctcaaggcca gcatgaattg ccgcttccac
     8881 gccggcacgg tgatttcgcg gatggtgtgg aatttatcga ccagcatctg gttgttggcc
     8941 tggatcatgc ggatggtcgg caggctctgc atggccgaat gttgcaaggc gatcaggtcg
     9001 ccgatgcgct tgtccaggtt ggcaaccatc gcatcgaggt cggccagctc ctgcacgcgg
     9061 ccagggtcgt tcccgacatt gccgcgcaga ccctcggcct gctcgcgcag ctcggcaagg
     9121 cggaccttgc cggccgcgat gtggacgcca agaaggcggt gttcctcgcg cacggctgcg
     9181 aacatttcgt cgagcgaggc attgcgctgc gcgatgcctt gctgggtggt ctgcacttcg
     9241 ctgaccaggt gttcgatctg ctcgcgggtc gtgtcgaagc gcgccatgaa gcccgtcgaa
     9301 cggacgcggg agcggtcgat cagcgggcca atcaggggca ggcgggaacg gttgtcggac
     9361 aaagggccga cgttcaggga acgggccttg gcgacaacct gggtcagttt ctcgcctgct
     9421 tcgtccaggt cgctgttgcg cacctggtcc agcaggctat cggcgtagcg ggacgtgtgc
     9481 tcggccacgt cgcggccgaa ctcggcaacg gtctgcggac tgccgacctc gatccgctgc
     9541 gcgaccgcat ggacttccgg cacgtcgctt tcctgcaagc ccagctcgcg cagggttgcc
     9601 ggggtcatgt caaaggcgac gataggggcc ttggcgtcgt gcgtcgtttt cagtgcgttc
     9661 atagggttct cccgccgtgt tattggttga tgccttccag gctctgcgaa aggctccgca
     9721 tgagcgcctg gtgagctttg gccgcctcgg cgaccattgc cggattcatg ttcttggtgg
     9781 tgatgagcgc gagggtgtgc tgacgccaga cgggcaccag gacggatgcc gtttcagaga
     9841 agcggtccag catgtccacg gcctgcgccc gcgtgagctt catctgagtg acgctcattt
     9901 catgggacgc catgagggtt gccaggttgg cgagcttgcg cgcgaagcgt tcgcgcggct
     9961 tgtcgaactc gatcacgccg gccttggccg cgccggcctc ggggttctcg tccaggaact
    10021 cgcgcccggc ttgaatgtag gctctgagcc ggtctacctc ggcctcatgc gtattgagca
    10081 tgtcatccaa ggcgcgcaac gtgtcccgca cgcgctgcgc tacgccctcg gcttcgtcca
    10141 gcaactggtc gagcgtcttg cgggcgacct gatacctcac ctggcgttca acctcacggc
    10201 caagcatctt ctcgaaccag gtaggctttt ccgcgatctt gcgggggtcc gcgtcgacca
//
