LOCUS       sequence-404867-       11285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB2-B-TelR
FEATURES             Location/Qualifiers
     source          1..11285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      382..439
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(463..480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(634..1222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(714..733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1405..1499)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1518..2312
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      2330..2416
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2432..3091)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3006..3025
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3253..3271
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3275..3318)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    complement(3373..3420)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     RBS             3512..3534
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(6435..6466)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(6530..6577)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    6744..6765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6780..6785,6786..6803,6804..6810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6818..6834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6852..6863
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6870..7547
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      7592..7663
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7679..7706
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 agcaacatgg tcgctgatat ggtcatactc agcatctttg acatagccgt attttgataa
       61 atatctttca tagtcctgcc gtttcttttt gtcatcttcc tgtttttgtt tagtttctct
      121 ttctatctgc tgttgttttc gttgctgttc ctgttgctcc tcttttatct ctctgtgata
      181 aatggaaagg ctagattgat cattatccca acgtgtgagc atgtcctcaa agccgtaata
      241 atcagcacta aaccaacctg ttttcacttt cactggtggc ggcagtggct ctccgatctc
      301 ccttagcagt gctgtagctg ctcgtccctc tttgatcact ttatcaagct gcaagcggtc
      361 gatattgtct tgcaactgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
      421 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      601 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1261 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1321 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1381 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1441 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1501 aagcgagctc gatataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     1561 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     1621 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     1681 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     1741 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     1801 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
     1861 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     1921 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     1981 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
     2041 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     2101 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     2161 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
     2221 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     2281 aatattatat tttactggat gaattgtttt aggacgtcgc cggcggcatc aaataaaacg
     2341 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2401 cctgagtagg acaaatcctc gagaaatcaa attacgcccc gccctgccac tcatcgcagt
     2461 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     2521 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     2581 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     2641 cccagggatt ggctgacacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     2701 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     2761 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     2821 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     2881 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     2941 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     3001 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     3061 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     3121 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     3181 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     3241 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     3301 ttcgctaagg atgatttctg gaattcgcgg ccgccccggg taccgagctc gaattagctt
     3361 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc
     3421 aagatcccct gattcccttt gtcaacagca atggatatca gccgttgact tagttggtat
     3481 ttccggaata tcatgctggg ttccgaataa ttttgtttaa ctttaagaag gagatatacc
     3541 atggaagaac aagcgtgaac atggcgcgat tgaaggggga ggttttgccc gccctcttcg
     3601 cgtcgccggc gacgattggc gagtacgggg ccggcatcga cggggcggat tccctcaacg
     3661 agctgtcgaa tctgatggag cacggcgcag ttgccgcgct ggccgacaaa atcagccaga
     3721 tcgtggcgaa gctggtcgac gcggaccccc gcaagatcgc ggaaaagcct acctggttcg
     3781 agaagatgct tggccgtgag gttgaacgcc aggtgaggta tcaggtcgcc cgcaagacgc
     3841 tcgaccagtt gctggacgaa gccgagggcg tagcgcagcg cgtgcgggac acgttgcgcg
     3901 ccttggatga catgctcaat acgcatgagg ccgaggtaga ccggctcaga gcctacattc
     3961 aagccgggcg cgagttcctg gacgagaacc ccgaggccgg cgcggccaag gccggcgtga
     4021 tcgagttcga caagccgcgc gaacgcttcg cgcgcaagct cgccaacctg gcaaccctca
     4081 tggcgtccca tgaaatgagc gtcactcaga tgaagctcac gcgggcgcag gccgtggaca
     4141 tgctggaccg cttctctgaa acggcatccg tcctggtgcc cgtctggcgt cagcacaccc
     4201 tcgcgctcat caccaccaag aacatgaatc cggcaatggt cgccgaggcg gccaaagctc
     4261 accaggcgct catgcggagc ctttcgcaga gcctggaagg catcaaccaa taacacggcg
     4321 ggagaaccct atgaacgcac tgaaaacgac gcacgacgcc aaggccccta tcgtcgcctt
     4381 tgacatgacc ccggcaaccc tgcgcgagct gggcttgcag gaaagcgacg tgccggaagt
     4441 ccatgcggtc gcgcagcgga tcgaggtcgg cagtccgcag accgttgccg agttcggccg
     4501 cgacgtggcc gagcacacgt cccgctacgc cgatagcctg ctggaccagg tgcgcaacag
     4561 cgacctggac gaagcaggcg agaaactgac ccaggttgtc gccaaggccc gttccctgaa
     4621 cgtcggccct ttgtccgaca accgttcccg cctgcccctg attggcccgc tgatcgaccg
     4681 ctcccgcgtc cgttcgacgg gcttcatggc gcgcttcgac acgacccgcg agcagatcga
     4741 acacctggtc agcgaagtgc agaccaccca gcaaggcatc gcgcagcgca atgcctcgct
     4801 cgacgaaatg ttcgcagccg tgcgcgagga acaccgcctt cttggcgtcc acatcgcggc
     4861 cggcaaggtc cgccttgccg agctgcgcga gcaggccgag ggtctgcgcg gcaatgtcgg
     4921 gaacgaccct ggccgcgtgc aggagctggc cgacctcgat gcgatggttg ccaacctgga
     4981 caagcgcatc ggcgacctga tcgccttgca acattcggcc atgcagagcc tgccgaccat
     5041 ccgcatgatc caggccaaca accagatgct ggtcgataaa ttccacacca tccgcgaaat
     5101 caccgtgccg gcgtggaagc ggcaattcat gctggccttg agcctcaacg agcagaagaa
     5161 cgccgtcgaa ctggccacgg ccatcgacga caccaccaac gacctgatga agcgcaatgc
     5221 ggccctgctg catcgcacgt ccgtcgaaac ggcgaaggag aaccaacgcc tggtgatcga
     5281 cgtggacacg ctcaagcagg ttcagacgac gctcatcaag accgtcgagg acgttattcg
     5341 catccagcag gaaggcgtgc agaagcgcaa ggatgccgag aagcagatcg ccgcaatgcg
     5401 tggcgatctt caagccaagc tgacccgcca gcccgtgcgc gagctggccc aacaggagtc
     5461 cgtatgaatg ccacaaacac cgatgttttc gcccaggtag gcggcctgga ggcccgaggc
     5521 gcgaagatga agaagcgggg cacccgcttc ctcatcgcgg cgctggcagt ccttgccatt
     5581 gccgggatcg gggcagtaac gggatgggcg atcagcccga gcgcgacgcc cggaagcatt
     5641 gacgtgccgc aggtgctggc atcgacattc agcgaccagg tgccgggcag tgagggcggc
     5701 ggcctgggtg gcggcctgcc cttcacttcg gccgtcgggg cattcacgga cttcatggcg
     5761 gggccggcaa tttttacctt gggcattctt ggcatagtgg tcgcgggtgc cgtgctcgtg
     5821 ttcgggggtg agttctgcgg gttcgtgcga tccgtctgca tgatggtgat agccgtcagc
     5881 atgattttcg tgtcgtcgaa cttggtgaag ggcattctcg gcggcgatca cgacgccggc
     5941 cctgcggagc cttcgccgcg tgcgcgattc atggcggccg tggaggccaa ggatttcgcg
     6001 cgagtgcaag agctgatcga ggcgcgtgga gccaagtcgg cggctgatta tgtccttgcg
     6061 cagctcgccg tggccgaagg tctggaccgc aagcctggtg cgcgcgtcgt ggtcgggaaa
     6121 gcggcgggca gcatggcaat gccgcctgcg gcgctgggtt ttacgccaag gggagaagcg
     6181 gcatacgcca tcgagcggtc agcctatggt gagccgaggt ccagcattgc gaagcagtac
     6241 cagcaggaat ggaaccggaa ggcggcgacc tggtgggcga tggccggtgt ggccggcatc
     6301 atcggcgcga tcctggcggc ggcggcaacc ggctttgttg ggctggcagt gtcgatccgc
     6361 aaccgagtga agcgcgtgcg cgacctgttg gtgatggagc cgggtgcaga gccataaggg
     6421 atcgatcccc ctcaagtcaa aagcctccgg tcggaggctt ttgactttct gctcgaggag
     6481 ctaattccat tgagtaagtt tttaagcaca tcagcttcaa aagcgctctg aagttcctat
     6541 actttctaga gaataggaac ttcggaatag gtacttcaag atccccaatt cgagctcggt
     6601 acccgggcgg ccgcttctag aggtctcagt acatctagag acgcaatacg caaaccgcct
     6661 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     6721 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     6781 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     6841 acatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     6901 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     6961 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     7021 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     7081 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     7141 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     7201 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     7261 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     7321 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     7381 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     7441 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     7501 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
     7561 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     7621 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     7681 ctcaccttcg ggtgggcctt tctgcgttta tacgtctcat tagggacaga gaccctgcag
     7741 ttaacaagtt gcctgacgct gtgtcttatc cagttcatgc ggcaatgcta tctctacttc
     7801 ctttgctaat cttgcctcct gtttgatgtc accgttcttt tttagttcgg attgctctac
     7861 ccgattccat agggtttgac gatctaacat gtcaggactt gccccagttg gggcaaaaat
     7921 ttgggtgtat tcaatgcctg tttttttggt gtagtcctgc tcttttccgt acgtatcaca
     7981 gtacaatttt tcgcctgctc ggtatgctgc acatgccacg attgagcgac catctgacct
     8041 cgaaatgttc tgcatttcac aatggtaaat tgccattttc atcacctgtt tattaacagc
     8101 cgccccaagt tttgggcggt tggggtgtcg gggtttccct gaccgtactt gcaaaatttg
     8161 gcgtagcaaa aattttgcgt aagtgcgccc ttcgggaact ctgcgaggct aaaagcaaaa
     8221 gcaagagcaa taaggctttg actttgtttt ttgattttgc tcgctgcgct cggatcttga
     8281 tgtaagtcga gtttttgaag tagaacactt ttcacattga tgatggttca tacttcggaa
     8341 aataggttga gacacaggca ttaaaaatgg tcaaaaaaac agctatggaa ttgggtcaaa
     8401 tgctcgatga agaaaaggaa aaattggaac gtcaggaaaa ggcaagaaag gatctagctg
     8461 acgctgtggt gaaaggtcga gagcaaaaac aggcaaggtc agacgatgca aaacgcaaga
     8521 tcctgatcgg ggcttactta ctgaagaaac atgataatga catcaagaaa cttgtggaac
     8581 aaaaccccga ttttattggg tacattagag aaaatgataa gcacttattt tcataggcat
     8641 tagtagttat aaaaaaccct cataaatcga gggtttcttt tttatctgca ttactttttg
     8701 aatcaatgta cttttttagc ttgctatttt tcactgcata cagccgctta tactctttct
     8761 caatcaatgc ggtcaaaatg gctttttggc tctcccctgc tgcgcccacc atgtccgcaa
     8821 gcatatccga cacaccctta tctataaaca cctccaaacg ttgactatca aggcttttgc
     8881 gtttttcacg atatgctttt tgtcgttcgg cattggtttt tggcggctct tttagcatgt
     8941 cctgtgtttt ctgatcgttc gggtctttca tgtctttaac tcccatatag tatatcatag
     9001 ttacaggata acattgtttt tagttacagg ataacttttt ataaaattta tttgtaaaaa
     9061 tttatgctat cctgcaacta tttttaaatt acaggataac tatttaacgt taccctgtaa
     9121 ctatagttat cctgtaacgc taaattaatt accctgtaac atatattttg ttatcctgta
     9181 acaactaaat attgaaataa tactaccaaa gttaccctgt aacgaaaata actaggttaa
     9241 aaaatgatcg gattttaaca ttttgcgttg ttccaaaagt tatcaacagc ctagaacgtc
     9301 ataggaagcg attacagaca ctttatagct atcagcatgg gaacataagg gcaggatgaa
     9361 atatgggtct taaaacgcaa atggtgaggt tttagaggta ttttttgaag atgattaagg
     9421 cggtttgttt ttaaaatttt tggcggctct caggctgctt acattttaac cagttcagtg
     9481 aaaagttctt tttcagcaaa tttctgttta gcaccatagc taaaacttgc gtggaacata
     9541 ttaagaattg accgaaatga cacaatctca attatatttt ttttgaaaag ttttctttat
     9601 caaatatttt aaatcattga tttatatata agtatacatt cattttaata attaatcttt
     9661 atttaacaat gatttatcta tattcaattg tttaattatt cttactaata ttatctctat
     9721 atcaatattt tttatttaaa aacatatgtt tagtagtgct tttgattaaa gtaccagagg
     9781 gagggagcag agctgaatgg gaaatactca ccctagagcg attcttaaaa atcaccctaa
     9841 agtattccca ttcgatgtac cgtcggtcgg tcgctttcgc atcagggatg acatcactgt
     9901 atcaagctgc cactgttatg attacgattg atagcaccgc ctgaacacgc tcataaccgc
     9961 caattaaatg actactcatt gcgtccgcta ctctgttcag ttcccctagt aatagcgttt
    10021 ttccgatgtg tgctagcgtc actgtacctc atcacccaca catggacaga ttgagttaca
    10081 gacattggct aaatttttgg ggtctatgct tgacaaagcg agttaaaacc ttagaattta
    10141 agacaggtac attaagcccc tgtggtgaaa tcattagcgg tcgtcaaacc ttaatgcttt
    10201 cgattgccga tatgtcagta tcgaagatct gtaccccata aattacggta aagccccaag
    10261 caattgcaag gggcttttat cttttttaac aaaaaaaatt tataaatcag gattttataa
    10321 cactaaataa tccaaagtac atgagtaagt catgaccact cctgcgatgt gtgtagcact
    10381 ctgagtatcc gtattatatc agtgatgtca tatacaacca cataatgcgg atgtatcact
    10441 aactccctag tatctttctg tctgcctgtg cgccccatca gtcgattatc aacaagtaaa
    10501 tctgtctttt cttcaattaa atcatcaatt tcaatagctg ctatagggtt gcgttcttca
    10561 agataatcat agatatttct acgatcttgg cgagctgttt cagtccactc aatcttcatt
    10621 cttcgctgcc agtttcgcca atgttgcagc acgtctagcg gcaaactctg cctttacgtc
    10681 ctcatgtgga atcaacttgc ctgcattggc ttcatttaag ccgatctgta cctgctgctt
    10741 gaaccatttg tcatagtctg cggcttcctg ttgcttctta acaaagtcac gcatgaagtc
    10801 acggattagc tgcgcccccg accgatcaac agtcttagca aggcttgaaa attcttgctt
    10861 taggtcatgg tctacccgaa aggtaaaagt cgcttctgtc atgtttttca ctccaatgtg
    10921 ttacatttga atgctattgt aacacattaa aaagttaggt cattgtcttt tttcggctta
    10981 ggctgttctt ggtcataacg tggtttaaat gatggctgtc gctcattctc aagtaaaatt
    11041 ttccgtttat taacagcctg ttgcatctgc tcatagtaag gcatcaactg cggtaaaata
    11101 tcagcaagtg cctgtctgtc tttatccagg atacggctca attgcccgat ttttttcaag
    11161 tcacgctcat aggctattct gtcgctgatc tgctcgtatt tttgctttga acaccttatg
    11221 tactcttgca tatcgttact tgcccatgcc tgcgcctgaa gacgtgtaaa catggaaatg
    11281 tctga
//
