LOCUS       sequence-404869-       11271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB2-D-TelR
FEATURES             Location/Qualifiers
     source          1..11271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(82..129)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     RBS             221..243
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(3144..3175)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(3239..3286)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      3333..3376
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(3380..3398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             3560..4219
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3626..3645)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4314..5108
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      5137..5231
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      5414..6002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5903..5922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6156..6173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6197..6254)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(10215..10242)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(10258..10329)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(10374..11051)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             11058..11069
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    11087..11103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11111..11117,11118..11135,11136..11141))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11156..11177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgcgtc tctagattcg atgagacctc tagaagcggc cgccccgggt accgagctcg
       61 aattagcttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat
      121 aggaacttca agatcccctg attccctttg tcaacagcaa tggatatcag ccgttgactt
      181 agttggtatt tccggaatat catgctgggt tccgaataat tttgtttaac tttaagaagg
      241 agatatacca tggaagaaca agcgtgaaca tggcgcgatt gaagggggag gttttgcccg
      301 ccctcttcgc gtcgccggcg acgattggcg agtacggggc cggcatcgac ggggcggatt
      361 ccctcaacga gctgtcgaat ctgatggagc acggcgcagt tgccgcgctg gccgacaaaa
      421 tcagccagat cgtggcgaag ctggtcgacg cggacccccg caagatcgcg gaaaagccta
      481 cctggttcga gaagatgctt ggccgtgagg ttgaacgcca ggtgaggtat caggtcgccc
      541 gcaagacgct cgaccagttg ctggacgaag ccgagggcgt agcgcagcgc gtgcgggaca
      601 cgttgcgcgc cttggatgac atgctcaata cgcatgaggc cgaggtagac cggctcagag
      661 cctacattca agccgggcgc gagttcctgg acgagaaccc cgaggccggc gcggccaagg
      721 ccggcgtgat cgagttcgac aagccgcgcg aacgcttcgc gcgcaagctc gccaacctgg
      781 caaccctcat ggcgtcccat gaaatgagcg tcactcagat gaagctcacg cgggcgcagg
      841 ccgtggacat gctggaccgc ttctctgaaa cggcatccgt cctggtgccc gtctggcgtc
      901 agcacaccct cgcgctcatc accaccaaga acatgaatcc ggcaatggtc gccgaggcgg
      961 ccaaagctca ccaggcgctc atgcggagcc tttcgcagag cctggaaggc atcaaccaat
     1021 aacacggcgg gagaacccta tgaacgcact gaaaacgacg cacgacgcca aggcccctat
     1081 cgtcgccttt gacatgaccc cggcaaccct gcgcgagctg ggcttgcagg aaagcgacgt
     1141 gccggaagtc catgcggtcg cgcagcggat cgaggtcggc agtccgcaga ccgttgccga
     1201 gttcggccgc gacgtggccg agcacacgtc ccgctacgcc gatagcctgc tggaccaggt
     1261 gcgcaacagc gacctggacg aagcaggcga gaaactgacc caggttgtcg ccaaggcccg
     1321 ttccctgaac gtcggccctt tgtccgacaa ccgttcccgc ctgcccctga ttggcccgct
     1381 gatcgaccgc tcccgcgtcc gttcgacggg cttcatggcg cgcttcgaca cgacccgcga
     1441 gcagatcgaa cacctggtca gcgaagtgca gaccacccag caaggcatcg cgcagcgcaa
     1501 tgcctcgctc gacgaaatgt tcgcagccgt gcgcgaggaa caccgccttc ttggcgtcca
     1561 catcgcggcc ggcaaggtcc gccttgccga gctgcgcgag caggccgagg gtctgcgcgg
     1621 caatgtcggg aacgaccctg gccgcgtgca ggagctggcc gacctcgatg cgatggttgc
     1681 caacctggac aagcgcatcg gcgacctgat cgccttgcaa cattcggcca tgcagagcct
     1741 gccgaccatc cgcatgatcc aggccaacaa ccagatgctg gtcgataaat tccacaccat
     1801 ccgcgaaatc accgtgccgg cgtggaagcg gcaattcatg ctggccttga gcctcaacga
     1861 gcagaagaac gccgtcgaac tggccacggc catcgacgac accaccaacg acctgatgaa
     1921 gcgcaatgcg gccctgctgc atcgcacgtc cgtcgaaacg gcgaaggaga accaacgcct
     1981 ggtgatcgac gtggacacgc tcaagcaggt tcagacgacg ctcatcaaga ccgtcgagga
     2041 cgttattcgc atccagcagg aaggcgtgca gaagcgcaag gatgccgaga agcagatcgc
     2101 cgcaatgcgt ggcgatcttc aagccaagct gacccgccag cccgtgcgcg agctggccca
     2161 acaggagtcc gtatgaatgc cacaaacacc gatgttttcg cccaggtagg cggcctggag
     2221 gcccgaggcg cgaagatgaa gaagcggggc acccgcttcc tcatcgcggc gctggcagtc
     2281 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc
     2341 ggaagcattg acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt
     2401 gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac
     2461 ttcatggcgg ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc
     2521 gtgctcgtgt tcgggggtga gttctgcggg ttcgtgcgat ccgtctgcat gatggtgata
     2581 gccgtcagca tgattttcgt gtcgtcgaac ttggtgaagg gcattctcgg cggcgatcac
     2641 gacgccggcc ctgcggagcc ttcgccgcgt gcgcgattca tggcggccgt ggaggccaag
     2701 gatttcgcgc gagtgcaaga gctgatcgag gcgcgtggag ccaagtcggc ggctgattat
     2761 gtccttgcgc agctcgccgt ggccgaaggt ctggaccgca agcctggtgc gcgcgtcgtg
     2821 gtcgggaaag cggcgggcag catggcaatg ccgcctgcgg cgctgggttt tacgccaagg
     2881 ggagaagcgg catacgccat cgagcggtca gcctatggtg agccgaggtc cagcattgcg
     2941 aagcagtacc agcaggaatg gaaccggaag gcggcgacct ggtgggcgat ggccggtgtg
     3001 gccggcatca tcggcgcgat cctggcggcg gcggcaaccg gctttgttgg gctggcagtg
     3061 tcgatccgca accgagtgaa gcgcgtgcgc gacctgttgg tgatggagcc gggtgcagag
     3121 ccataaggga tcgatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg
     3181 ctcgaggagc taattccatt gagtaagttt ttaagcacat cagcttcaaa agcgctctga
     3241 agttcctata ctttctagag aataggaact tcggaatagg tacttcaaga tccccaattc
     3301 gagctcggta cccggggcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat
     3361 tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3421 ataatggttt cttagacgtc aggtggcact cgagttgatc gggcacgtaa gaggttccaa
     3481 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     3541 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     3601 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     3661 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     3721 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg
     3781 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     3841 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     3901 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     3961 ccctaaaggg tttattgaga atatgttttt cgtgtcagcc aatccctggg tgagtttcac
     4021 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     4081 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     4141 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     4201 gtggcagggc ggggcgtaat ttgatttctc gagaagctgg ggatccgttt gatttttaat
     4261 ggataatgtg atataatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     4321 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     4381 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     4441 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     4501 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     4561 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     4621 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     4681 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     4741 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     4801 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     4861 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     4921 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     4981 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     5041 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     5101 tgttttagga cgtcgccggc ggatcgagct cgcttggact cctgttgata gatccagtaa
     5161 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     5221 ttggtgagaa tccaagcctc gagctgtcag accaagttta ctcatatata ctttagattg
     5281 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5341 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5401 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5461 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5521 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5581 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5641 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5701 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5761 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5821 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5881 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5941 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctgtgga
     6001 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6061 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
     6181 gcataacgcg aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc
     6241 ccttttttgc cggactgcag ttaacaagtt gcctgacgct gtgtcttatc cagttcatgc
     6301 ggcaatgcta tctctacttc ctttgctaat cttgcctcct gtttgatgtc accgttcttt
     6361 tttagttcgg attgctctac ccgattccat agggtttgac gatctaacat gtcaggactt
     6421 gccccagttg gggcaaaaat ttgggtgtat tcaatgcctg tttttttggt gtagtcctgc
     6481 tcttttccgt acgtatcaca gtacaatttt tcgcctgctc ggtatgctgc acatgccacg
     6541 attgagcgac catctgacct cgaaatgttc tgcatttcac aatggtaaat tgccattttc
     6601 atcacctgtt tattaacagc cgccccaagt tttgggcggt tggggtgtcg gggtttccct
     6661 gaccgtactt gcaaaatttg gcgtagcaaa aattttgcgt aagtgcgccc ttcgggaact
     6721 ctgcgaggct aaaagcaaaa gcaagagcaa taaggctttg actttgtttt ttgattttgc
     6781 tcgctgcgct cggatcttga tgtaagtcga gtttttgaag tagaacactt ttcacattga
     6841 tgatggttca tacttcggaa aataggttga gacacaggca ttaaaaatgg tcaaaaaaac
     6901 agctatggaa ttgggtcaaa tgctcgatga agaaaaggaa aaattggaac gtcaggaaaa
     6961 ggcaagaaag gatctagctg acgctgtggt gaaaggtcga gagcaaaaac aggcaaggtc
     7021 agacgatgca aaacgcaaga tcctgatcgg ggcttactta ctgaagaaac atgataatga
     7081 catcaagaaa cttgtggaac aaaaccccga ttttattggg tacattagag aaaatgataa
     7141 gcacttattt tcataggcat tagtagttat aaaaaaccct cataaatcga gggtttcttt
     7201 tttatctgca ttactttttg aatcaatgta cttttttagc ttgctatttt tcactgcata
     7261 cagccgctta tactctttct caatcaatgc ggtcaaaatg gctttttggc tctcccctgc
     7321 tgcgcccacc atgtccgcaa gcatatccga cacaccctta tctataaaca cctccaaacg
     7381 ttgactatca aggcttttgc gtttttcacg atatgctttt tgtcgttcgg cattggtttt
     7441 tggcggctct tttagcatgt cctgtgtttt ctgatcgttc gggtctttca tgtctttaac
     7501 tcccatatag tatatcatag ttacaggata acattgtttt tagttacagg ataacttttt
     7561 ataaaattta tttgtaaaaa tttatgctat cctgcaacta tttttaaatt acaggataac
     7621 tatttaacgt taccctgtaa ctatagttat cctgtaacgc taaattaatt accctgtaac
     7681 atatattttg ttatcctgta acaactaaat attgaaataa tactaccaaa gttaccctgt
     7741 aacgaaaata actaggttaa aaaatgatcg gattttaaca ttttgcgttg ttccaaaagt
     7801 tatcaacagc ctagaacgtc ataggaagcg attacagaca ctttatagct atcagcatgg
     7861 gaacataagg gcaggatgaa atatgggtct taaaacgcaa atggtgaggt tttagaggta
     7921 ttttttgaag atgattaagg cggtttgttt ttaaaatttt tggcggctct caggctgctt
     7981 acattttaac cagttcagtg aaaagttctt tttcagcaaa tttctgttta gcaccatagc
     8041 taaaacttgc gtggaacata ttaagaattg accgaaatga cacaatctca attatatttt
     8101 ttttgaaaag ttttctttat caaatatttt aaatcattga tttatatata agtatacatt
     8161 cattttaata attaatcttt atttaacaat gatttatcta tattcaattg tttaattatt
     8221 cttactaata ttatctctat atcaatattt tttatttaaa aacatatgtt tagtagtgct
     8281 tttgattaaa gtaccagagg gagggagcag agctgaatgg gaaatactca ccctagagcg
     8341 attcttaaaa atcaccctaa agtattccca ttcgatgtac cgtcggtcgg tcgctttcgc
     8401 atcagggatg acatcactgt atcaagctgc cactgttatg attacgattg atagcaccgc
     8461 ctgaacacgc tcataaccgc caattaaatg actactcatt gcgtccgcta ctctgttcag
     8521 ttcccctagt aatagcgttt ttccgatgtg tgctagcgtc actgtacctc atcacccaca
     8581 catggacaga ttgagttaca gacattggct aaatttttgg ggtctatgct tgacaaagcg
     8641 agttaaaacc ttagaattta agacaggtac attaagcccc tgtggtgaaa tcattagcgg
     8701 tcgtcaaacc ttaatgcttt cgattgccga tatgtcagta tcgaagatct gtaccccata
     8761 aattacggta aagccccaag caattgcaag gggcttttat cttttttaac aaaaaaaatt
     8821 tataaatcag gattttataa cactaaataa tccaaagtac atgagtaagt catgaccact
     8881 cctgcgatgt gtgtagcact ctgagtatcc gtattatatc agtgatgtca tatacaacca
     8941 cataatgcgg atgtatcact aactccctag tatctttctg tctgcctgtg cgccccatca
     9001 gtcgattatc aacaagtaaa tctgtctttt cttcaattaa atcatcaatt tcaatagctg
     9061 ctatagggtt gcgttcttca agataatcat agatatttct acgatcttgg cgagctgttt
     9121 cagtccactc aatcttcatt cttcgctgcc agtttcgcca atgttgcagc acgtctagcg
     9181 gcaaactctg cctttacgtc ctcatgtgga atcaacttgc ctgcattggc ttcatttaag
     9241 ccgatctgta cctgctgctt gaaccatttg tcatagtctg cggcttcctg ttgcttctta
     9301 acaaagtcac gcatgaagtc acggattagc tgcgcccccg accgatcaac agtcttagca
     9361 aggcttgaaa attcttgctt taggtcatgg tctacccgaa aggtaaaagt cgcttctgtc
     9421 atgtttttca ctccaatgtg ttacatttga atgctattgt aacacattaa aaagttaggt
     9481 cattgtcttt tttcggctta ggctgttctt ggtcataacg tggtttaaat gatggctgtc
     9541 gctcattctc aagtaaaatt ttccgtttat taacagcctg ttgcatctgc tcatagtaag
     9601 gcatcaactg cggtaaaata tcagcaagtg cctgtctgtc tttatccagg atacggctca
     9661 attgcccgat ttttttcaag tcacgctcat aggctattct gtcgctgatc tgctcgtatt
     9721 tttgctttga acaccttatg tactcttgca tatcgttact tgcccatgcc tgcgcctgaa
     9781 gacgtgtaaa catggaaatg tctgaagcaa catggtcgct gatatggtca tactcagcat
     9841 ctttgacata gccgtatttt gataaatatc tttcatagtc ctgccgtttc tttttgtcat
     9901 cttcctgttt ttgtttagtt tctctttcta tctgctgttg ttttcgttgc tgttcctgtt
     9961 gctcctcttt tatctctctg tgataaatgg aaaggctaga ttgatcatta tcccaacgtg
    10021 tgagcatgtc ctcaaagccg taataatcag cactaaacca acctgttttc actttcactg
    10081 gtggcggcag tggctctccg atctccctta gcagtgctgt agctgctcgt ccctctttga
    10141 tcactttatc aagctgcaag cggtcgatat tgtcttgcaa ctgcagggtc tctaacacta
    10201 atgagacgta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag
    10261 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
    10321 ttttatttga tgcctggctc tagtagcgat ctacactagc actatcagcg ttattaagca
    10381 ccggtggagt gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg
    10441 tgggaggtga tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt
    10501 ttagccatgt aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga
    10561 cgcattttga tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct
    10621 tcccaaccca tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc
    10681 agtttaactt tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca
    10741 acaccaccgt cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt
    10801 ttcaggtagt ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac
    10861 tgcggggaca ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta
    10921 gcggtctggg taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga
    10981 ccgttaacgg aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg
    11041 gaggaagcca tctagtattt ctcctctttc tctagtatgt gtgaaattgt tatccgctca
    11101 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    11161 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    11221 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg c
//
