LOCUS       sequence-404870-       11390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB2-A-TelR
FEATURES             Location/Qualifiers
     source          1..11390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(49..80)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(144..191)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    371..392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(407..412,413..430,431..437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    445..461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             479..490
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             497..1174
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1219..1290
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1306..1333
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5300..5357
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(5381..5398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5552..6140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5632..5651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6323..6417)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(6442..6528)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6546..7340)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             complement(7436..8095)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8010..8029
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     8257..8275
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8279..8322)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    complement(8377..8424)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     RBS             8516..8538
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gttggtgatg gagccgggtg cagagccata agggatcgat ccccctcaag tcaaaagcct
       61 ccggtcggag gcttttgact ttctgctcga ggagctaatt ccattgagta agtttttaag
      121 cacatcagct tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
      181 ataggtactt caagatcccc aattcgagct cggtacccgg ggcggccgct tctagaggtc
      241 tcactatatc tagagacgtc tagacatatg caatacgcaa accgcctctc cccgcgcgtt
      301 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      361 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      421 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa
      481 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
      541 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      601 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      661 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      721 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      781 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      841 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
      901 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
      961 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
     1021 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
     1081 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
     1141 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
     1201 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1261 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1321 gggcctttct gcgtttatag catgccgtct cattaggtac agagaccctg cagttgcaag
     1381 acaatatcga ccgcttgcag cttgataaag tgatcaaaga gggacgagca gctacagcac
     1441 tgctaaggga gatcggagag ccactgccgc caccagtgaa agtgaaaaca ggttggttta
     1501 gtgctgatta ttacggcttt gaggacatgc tcacacgttg ggataatgat caatctagcc
     1561 tttccattta tcacagagag ataaaagagg agcaacagga acagcaacga aaacaacagc
     1621 agatagaaag agaaactaaa caaaaacagg aagatgacaa aaagaaacgg caggactatg
     1681 aaagatattt atcaaaatac ggctatgtca aagatgctga gtatgaccat atcagcgacc
     1741 atgttgcttc agacatttcc atgtttacac gtcttcaggc gcaggcatgg gcaagtaacg
     1801 atatgcaaga gtacataagg tgttcaaagc aaaaatacga gcagatcagc gacagaatag
     1861 cctatgagcg tgacttgaaa aaaatcgggc aattgagccg tatcctggat aaagacagac
     1921 aggcacttgc tgatatttta ccgcagttga tgccttacta tgagcagatg caacaggctg
     1981 ttaataaacg gaaaatttta cttgagaatg agcgacagcc atcatttaaa ccacgttatg
     2041 accaagaaca gcctaagccg aaaaaagaca atgacctaac tttttaatgt gttacaatag
     2101 cattcaaatg taacacattg gagtgaaaaa catgacagaa gcgactttta cctttcgggt
     2161 agaccatgac ctaaagcaag aattttcaag ccttgctaag actgttgatc ggtcgggggc
     2221 gcagctaatc cgtgacttca tgcgtgactt tgttaagaag caacaggaag ccgcagacta
     2281 tgacaaatgg ttcaagcagc aggtacagat cggcttaaat gaagccaatg caggcaagtt
     2341 gattccacat gaggacgtaa aggcagagtt tgccgctaga cgtgctgcaa cattggcgaa
     2401 actggcagcg aagaatgaag attgagtgga ctgaaacagc tcgccaagat cgtagaaata
     2461 tctatgatta tcttgaagaa cgcaacccta tagcagctat tgaaattgat gatttaattg
     2521 aagaaaagac agatttactt gttgataatc gactgatggg gcgcacaggc agacagaaag
     2581 atactaggga gttagtgata catccgcatt atgtggttgt atatgacatc actgatataa
     2641 tacggatact cagagtgcta cacacatcgc aggagtggtc atgacttact catgtacttt
     2701 ggattattta gtgttataaa atcctgattt ataaattttt tttgttaaaa aagataaaag
     2761 ccccttgcaa ttgcttgggg ctttaccgta atttatgggg tacagatctt cgatactgac
     2821 atatcggcaa tcgaaagcat taaggtttga cgaccgctaa tgatttcacc acaggggctt
     2881 aatgtacctg tcttaaattc taaggtttta actcgctttg tcaagcatag accccaaaaa
     2941 tttagccaat gtctgtaact caatctgtcc atgtgtgggt gatgaggtac agtgacgcta
     3001 gcacacatcg gaaaaacgct attactaggg gaactgaaca gagtagcgga cgcaatgagt
     3061 agtcatttaa ttggcggtta tgagcgtgtt caggcggtgc tatcaatcgt aatcataaca
     3121 gtggcagctt gatacagtga tgtcatccct gatgcgaaag cgaccgaccg acggtacatc
     3181 gaatgggaat actttagggt gatttttaag aatcgctcta gggtgagtat ttcccattca
     3241 gctctgctcc ctccctctgg tactttaatc aaaagcacta ctaaacatat gtttttaaat
     3301 aaaaaatatt gatatagaga taatattagt aagaataatt aaacaattga atatagataa
     3361 atcattgtta aataaagatt aattattaaa atgaatgtat acttatatat aaatcaatga
     3421 tttaaaatat ttgataaaga aaacttttca aaaaaaatat aattgagatt gtgtcatttc
     3481 ggtcaattct taatatgttc cacgcaagtt ttagctatgg tgctaaacag aaatttgctg
     3541 aaaaagaact tttcactgaa ctggttaaaa tgtaagcagc ctgagagccg ccaaaaattt
     3601 taaaaacaaa ccgccttaat catcttcaaa aaatacctct aaaacctcac catttgcgtt
     3661 ttaagaccca tatttcatcc tgcccttatg ttcccatgct gatagctata aagtgtctgt
     3721 aatcgcttcc tatgacgttc taggctgttg ataacttttg gaacaacgca aaatgttaaa
     3781 atccgatcat tttttaacct agttattttc gttacagggt aactttggta gtattatttc
     3841 aatatttagt tgttacagga taacaaaata tatgttacag ggtaattaat ttagcgttac
     3901 aggataacta tagttacagg gtaacgttaa atagttatcc tgtaatttaa aaatagttgc
     3961 aggatagcat aaatttttac aaataaattt tataaaaagt tatcctgtaa ctaaaaacaa
     4021 tgttatcctg taactatgat atactatatg ggagttaaag acatgaaaga cccgaacgat
     4081 cagaaaacac aggacatgct aaaagagccg ccaaaaacca atgccgaacg acaaaaagca
     4141 tatcgtgaaa aacgcaaaag ccttgatagt caacgtttgg aggtgtttat agataagggt
     4201 gtgtcggata tgcttgcgga catggtgggc gcagcagggg agagccaaaa agccattttg
     4261 accgcattga ttgagaaaga gtataagcgg ctgtatgcag tgaaaaatag caagctaaaa
     4321 aagtacattg attcaaaaag taatgcagat aaaaaagaaa ccctcgattt atgagggttt
     4381 tttataacta ctaatgccta tgaaaataag tgcttatcat tttctctaat gtacccaata
     4441 aaatcggggt tttgttccac aagtttcttg atgtcattat catgtttctt cagtaagtaa
     4501 gccccgatca ggatcttgcg ttttgcatcg tctgaccttg cctgtttttg ctctcgacct
     4561 ttcaccacag cgtcagctag atcctttctt gccttttcct gacgttccaa tttttccttt
     4621 tcttcatcga gcatttgacc caattccata gctgtttttt tgaccatttt taatgcctgt
     4681 gtctcaacct attttccgaa gtatgaacca tcatcaatgt gaaaagtgtt ctacttcaaa
     4741 aactcgactt acatcaagat ccgagcgcag cgagcaaaat caaaaaacaa agtcaaagcc
     4801 ttattgctct tgcttttgct tttagcctcg cagagttccc gaagggcgca cttacgcaaa
     4861 atttttgcta cgccaaattt tgcaagtacg gtcagggaaa ccccgacacc ccaaccgccc
     4921 aaaacttggg gcggctgtta ataaacaggt gatgaaaatg gcaatttacc attgtgaaat
     4981 gcagaacatt tcgaggtcag atggtcgctc aatcgtggca tgtgcagcat accgagcagg
     5041 cgaaaaattg tactgtgata cgtacggaaa agagcaggac tacaccaaaa aaacaggcat
     5101 tgaatacacc caaatttttg ccccaactgg ggcaagtcct gacatgttag atcgtcaaac
     5161 cctatggaat cgggtagagc aatccgaact aaaaaagaac ggtgacatca aacaggaggc
     5221 aagattagca aaggaagtag agatagcatt gccgcatgaa ctggataaga cacagcgtca
     5281 ggcaacttgt taactgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt
     5341 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc
     5401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
     5581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6241 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6301 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     6361 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     6421 gcgagctcga tattctcgag gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     6481 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gccgccggcg
     6541 acgtcctaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     6601 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     6661 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     6721 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     6781 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     6841 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     6901 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     6961 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     7021 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     7081 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     7141 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     7201 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     7261 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     7321 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatta
     7381 tatcacatta tccattaaaa atcaaacgga tccccagctt ctcgagaata tcaaattacg
     7441 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     7501 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     7561 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     7621 ttaaatcaaa actggtgaaa ctcacccagg gattggctga cacgaaaaac atattctcaa
     7681 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     7741 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     7801 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     7861 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     7921 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     7981 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     8041 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     8101 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     8161 cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc
     8221 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     8281 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgccc
     8341 cgggtaccga gctcgaatta gcttcaaaag cgctctgaag ttcctatact ttctagagaa
     8401 taggaacttc ggaataggaa cttcaagatc ccctgattcc ctttgtcaac agcaatggat
     8461 atcagccgtt gacttagttg gtatttccgg aatatcatgc tgggttccga ataattttgt
     8521 ttaactttaa gaaggagata taccatggaa gaacaagcgt gaacatggcg cgattgaagg
     8581 gggaggtttt gcccgccctc ttcgcgtcgc cggcgacgat tggcgagtac ggggccggca
     8641 tcgacggggc ggattccctc aacgagctgt cgaatctgat ggagcacggc gcagttgccg
     8701 cgctggccga caaaatcagc cagatcgtgg cgaagctggt cgacgcggac ccccgcaaga
     8761 tcgcggaaaa gcctacctgg ttcgagaaga tgcttggccg tgaggttgaa cgccaggtga
     8821 ggtatcaggt cgcccgcaag acgctcgacc agttgctgga cgaagccgag ggcgtagcgc
     8881 agcgcgtgcg ggacacgttg cgcgccttgg atgacatgct caatacgcat gaggccgagg
     8941 tagaccggct cagagcctac attcaagccg ggcgcgagtt cctggacgag aaccccgagg
     9001 ccggcgcggc caaggccggc gtgatcgagt tcgacaagcc gcgcgaacgc ttcgcgcgca
     9061 agctcgccaa cctggcaacc ctcatggcgt cccatgaaat gagcgtcact cagatgaagc
     9121 tcacgcgggc gcaggccgtg gacatgctgg accgcttctc tgaaacggca tccgtcctgg
     9181 tgcccgtctg gcgtcagcac accctcgcgc tcatcaccac caagaacatg aatccggcaa
     9241 tggtcgccga ggcggccaaa gctcaccagg cgctcatgcg gagcctttcg cagagcctgg
     9301 aaggcatcaa ccaataacac ggcgggagaa ccctatgaac gcactgaaaa cgacgcacga
     9361 cgccaaggcc cctatcgtcg cctttgacat gaccccggca accctgcgcg agctgggctt
     9421 gcaggaaagc gacgtgccgg aagtccatgc ggtcgcgcag cggatcgagg tcggcagtcc
     9481 gcagaccgtt gccgagttcg gccgcgacgt ggccgagcac acgtcccgct acgccgatag
     9541 cctgctggac caggtgcgca acagcgacct ggacgaagca ggcgagaaac tgacccaggt
     9601 tgtcgccaag gcccgttccc tgaacgtcgg ccctttgtcc gacaaccgtt cccgcctgcc
     9661 cctgattggc ccgctgatcg accgctcccg cgtccgttcg acgggcttca tggcgcgctt
     9721 cgacacgacc cgcgagcaga tcgaacacct ggtcagcgaa gtgcagacca cccagcaagg
     9781 catcgcgcag cgcaatgcct cgctcgacga aatgttcgca gccgtgcgcg aggaacaccg
     9841 ccttcttggc gtccacatcg cggccggcaa ggtccgcctt gccgagctgc gcgagcaggc
     9901 cgagggtctg cgcggcaatg tcgggaacga ccctggccgc gtgcaggagc tggccgacct
     9961 cgatgcgatg gttgccaacc tggacaagcg catcggcgac ctgatcgcct tgcaacattc
    10021 ggccatgcag agcctgccga ccatccgcat gatccaggcc aacaaccaga tgctggtcga
    10081 taaattccac accatccgcg aaatcaccgt gccggcgtgg aagcggcaat tcatgctggc
    10141 cttgagcctc aacgagcaga agaacgccgt cgaactggcc acggccatcg acgacaccac
    10201 caacgacctg atgaagcgca atgcggccct gctgcatcgc acgtccgtcg aaacggcgaa
    10261 ggagaaccaa cgcctggtga tcgacgtgga cacgctcaag caggttcaga cgacgctcat
    10321 caagaccgtc gaggacgtta ttcgcatcca gcaggaaggc gtgcagaagc gcaaggatgc
    10381 cgagaagcag atcgccgcaa tgcgtggcga tcttcaagcc aagctgaccc gccagcccgt
    10441 gcgcgagctg gcccaacagg agtccgtatg aatgccacaa acaccgatgt tttcgcccag
    10501 gtaggcggcc tggaggcccg aggcgcgaag atgaagaagc ggggcacccg cttcctcatc
    10561 gcggcgctgg cagtccttgc cattgccggg atcggggcag taacgggatg ggcgatcagc
    10621 ccgagcgcga cgcccggaag cattgacgtg ccgcaggtgc tggcatcgac attcagcgac
    10681 caggtgccgg gcagtgaggg cggcggcctg ggtggcggcc tgcccttcac ttcggccgtc
    10741 ggggcattca cggacttcat ggcggggccg gcaattttta ccttgggcat tcttggcata
    10801 gtggtcgcgg gtgccgtgct cgtgttcggg ggtgagttct gcgggttcgt gcgatccgtc
    10861 tgcatgatgg tgatagccgt cagcatgatt ttcgtgtcgt cgaacttggt gaagggcatt
    10921 ctcggcggcg atcacgacgc cggccctgcg gagccttcgc cgcgtgcgcg attcatggcg
    10981 gccgtggagg ccaaggattt cgcgcgagtg caagagctga tcgaggcgcg tggagccaag
    11041 tcggcggctg attatgtcct tgcgcagctc gccgtggccg aaggtctgga ccgcaagcct
    11101 ggtgcgcgcg tcgtggtcgg gaaagcggcg ggcagcatgg caatgccgcc tgcggcgctg
    11161 ggttttacgc caaggggaga agcggcatac gccatcgagc ggtcagccta tggtgagccg
    11221 aggtccagca ttgcgaagca gtaccagcag gaatggaacc ggaaggcggc gacctggtgg
    11281 gcgatggccg gtgtggccgg catcatcggc gcgatcctgg cggcggcggc aaccggcttt
    11341 gttgggctgg cagtgtcgat ccgcaaccga gtgaagcgcg tgcgcgacct
//
