LOCUS       sequence-404874-       10259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cytosine base editing using miniSdd7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, 
            Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT,
            Gao C
  TITLE     Discovery of deaminase functions by structure-based protein 
            clustering.
  JOURNAL   Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 
            10.1016/j.cell.2023.05.041. Epub 2023 Jun 27.
  PUBMED    37379837
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.05.041"; journalName: "Cell"; date: 
            "2023-07-20- 20"; volume: "186"; issue: "15"; pages: "3182-3195.e14"
COMMENT     Sequence Label: p2T-CMV-miniSdd7-BE4max-BlastR
FEATURES             Location/Qualifiers
     source          1..10259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(902..925,926..928,929..5002)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             902..5002
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5033..5281
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5312..5560
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5603..5623
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5632..5649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5672..5689)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5678..5902
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5973..5989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5973..5989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5986..6008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5997..6013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6021..6027,6028..6045,6046..6051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6066..6087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6204..6325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6215..6234)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6269..6288
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6483..6881)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6900..6947)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6995..7324)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7009..7144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7063..7082)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7309..7329
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7448..7635,7636..7647)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7708..8296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7788..7807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8467..9258,9259..9327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9090..9109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9328..9432)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9511..9530)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9722..9733,9734..9871)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9880..10259
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtcgaagga ggtggccctg gggcagttcc
      361 tgagggtggc gacggcccac ctgccgtccc tgccgaggag gtggaaagac tgcgcggaga
      421 gctgcctccc cctgtggtgc cagggactgg gcaaaagacc cacggcagat ggatcggccc
      481 tgacggtaga gtccgggcca tcgtgagcgg aagagatgaa gatgctgcac tggtgcacgc
      541 ccagctggct gccaagggca tcccagacga gcccaccagg aactctgatg tggagcagaa
      601 gctggcagcc cacatggtgg cgaatgggat caggcatgtg accctggtta tcaaccacag
      661 gccttgcaga ggcttcgatg actcctgtga taccctggtg cctatcattc tgccagaagg
      721 ctgcacactg accgtgcatg gccagacaga caaaggcatg agagtccgag tgcgctacac
      781 aggaggagcc cggccctggt ggagctccgg aggatctagc ggaggctcct ctggctctga
      841 gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc
      901 agacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
      961 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     1021 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     1081 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     1141 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     1201 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     1261 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     1321 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     1381 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     1441 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     1501 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     1561 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     1621 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     1681 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     1741 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     1801 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     1861 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     1921 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     1981 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     2041 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     2101 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     2161 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     2221 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     2281 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     2341 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     2401 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     2461 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     2521 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     2581 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     2641 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     2701 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     2761 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     2821 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     2881 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     2941 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     3001 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     3061 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     3121 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     3181 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     3241 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     3301 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     3361 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     3421 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     3481 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     3541 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     3601 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     3661 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     3721 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtaatcaccc tgaagtccaa
     3781 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     3841 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     3901 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     3961 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     4021 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     4081 tctaatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     4141 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     4201 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     4261 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     4321 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     4381 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     4441 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     4501 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     4561 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     4621 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     4681 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     4741 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     4801 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     4861 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     4921 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     4981 cctgtctcag ctgggaggtg acagcggcgg gagcggcggg agcgggggga gcactaatct
     5041 gagcgacatc attgagaagg agactgggaa acagctggtc attcaggagt ccatcctgat
     5101 gctgcctgag gaggtggagg aagtgatcgg caacaagcca gagtctgaca tcctggtgca
     5161 caccgcctac gacgagtcca cagatgagaa tgtgatgctg ctgacctctg acgcccccga
     5221 gtataagcct tgggccctgg tcatccagga ttctaacggc gagaataaga tcaagatgct
     5281 gagcggagga tccggaggat ctggaggcag caccaacctg tctgacatca tcgagaagga
     5341 gacaggcaag cagctggtca tccaggagag catcctgatg ctgcccgaag aagtcgaaga
     5401 agtgatcgga aacaagcctg agagcgatat cctggtccat accgcctacg acgagagtac
     5461 cgacgaaaat gtgatgctgc tgacatccga cgccccagag tataagccct gggctctggt
     5521 catccaggat tccaacggag agaacaaaat caaaatgctg tctggcggct caaaaagaac
     5581 cgccgacggc agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg tcatcatcac
     5641 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5701 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5761 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5821 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5881 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     5941 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6001 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt
     6061 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6121 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6181 cgtattgggc gctacagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6241 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6301 cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc
     6361 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     6421 aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc ctgctcctcg gccacgaagt
     6481 gcttagccct cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc
     6541 tgtccatcac tgtccttcac tatggctttg atcccaggat gcagatcgag aagcacctgt
     6601 cggcaccgtc cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa
     6661 gtcaggttgc cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcacaaggt
     6721 cccccagtaa aatgatatac attgacacca gtaaagatgc ggccgtcgct agagagagct
     6781 gcgctggcga cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt
     6841 tcaatgaggg tggattcttc ttgagacaaa ggcttggcca tggtttagtt cctcaccttg
     6901 tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacg tgctgatcag
     6961 atccgaaaat ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa
     7021 agcctcctca ctacttctgg aatagctcag aggcaaaggc ggcctcggcc tctgcataaa
     7081 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     7141 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     7201 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     7261 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     7321 acacattcca cagaattcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7381 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7441 aacatgtaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     7501 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     7561 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     7621 aaattactca agtactttac acctctgcat gtgagcaaaa ggccagcaaa aggccaggaa
     7681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa
     8341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8581 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     8641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9421 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg
     9481 atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag
     9541 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     9601 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     9661 ttttgcgctg cttcgcgatg tacgggccag atatacgcgg gtaccggcat atggttcttg
     9721 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
     9781 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
     9841 aagtactcca ttaaaattgt acttgagtat taacgcgttg acattgatta ttgactagtt
     9901 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     9961 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10021 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10081 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    10141 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    10201 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
