LOCUS       sequence-404886-        3735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT5G37830(B,E)
FEATURES             Location/Qualifiers
     source          1..3735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      284..872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     773..792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1026..1043
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2645..2663)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2731..2835
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3057..3735,1..113)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
       61 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
      121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
      181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
      241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      961 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1021 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     1081 ccgcgcgttg gccgattcat taatcactct gtggtctcag gaggaaagtt cgagtcaaat
     1141 ttttactacc gcccatgagc tataatttta acatgcgtcc accaatgtct caattaaata
     1201 tgcttaagct atccaatccc aatatatatc atagtgtagt agctaattac gctaactcaa
     1261 tcctaatgta tcaaacttta ttaggctaat tagatccgga tccaaaattc aatcggatat
     1321 ccggatgcga tagattcgaa tctgagtaat agtaaaaatc aaaattggga tatcacggtt
     1381 attggcctac tttcgcgcta aacccaatat cctgatccgg tctaatagat aaacatcgca
     1441 cattcacata gtggtgggct taaaatacag atgatgtacc tatctactag gaaattcgag
     1501 aaaagatgga ataggaaaga cgtgtttcca aataatccta tgactaacct agctcaaaaa
     1561 ttttaggaca aaaaacggaa tccgcgggat aaaaggggga tggcagtctc atgtccccct
     1621 tgccggtggg gactctgctc ccgggttggt gcccatgttt cgagaagttt aaggcaaatg
     1681 gtgtgcccat gttttgagag gtttaaggca acttgtaact atatgacatt ttagtttaaa
     1741 catatatccc aaacctagag tgtgcctata aaagaaggaa aattgaaatt aaagctttaa
     1801 ttagtcattt ttcgtccttt aaaaaagaag aagagtgaaa aagatatatg tttttttgta
     1861 atttttttac ttaacactca attttttggt tttaacaaaa tatgatttaa aatactagaa
     1921 aatcattata attagtgtat tttacaattt tatattttcc caacatgtaa caaaaaaaaa
     1981 cgatttttct tatgtgcata ttagtatgta tgtaaagttt cgtaatatgg atatacacat
     2041 ctgttttaaa aggggaaaag agtattggtg gttataggag atttcatcat ataatctaat
     2101 tatattaatt aaaaagtaaa taaacaataa atttatatat ctcaagctag actctagtga
     2161 ggataattcg ttattcaaaa tcgaatatcc cgatccggtc cagtagataa cattacacaa
     2221 attaggccaa cacattcatt cataccgtgg ggtttatgat tatatccgtt ttaaattcta
     2281 accaaccaaa aaccgaaatg gtttagtaaa accggaaaag caaaagaaga tccttcacat
     2341 tctcatcatc atcaccacct gattcatctt cttcatcatt cacccactac actgttgact
     2401 cttcgctaaa actcgatcct tcaacgatct tcgccaccaa agcaaggtac tttcataaat
     2461 atctggattt tgattacaaa ccccttctct cgtagtttca ttttaatcga tactatctaa
     2521 aattgctgtt atgatctctc aatatcgttg ttgtgaattg gtaatttagt gtaatttcgt
     2581 tgttgggtta tgcagagaga gtgagaatgt gagaccacga agtggctctt cagtggacga
     2641 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2701 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2761 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2821 tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     2881 ggtaacggcg cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt
     2941 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     3001 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
     3061 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     3121 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     3181 tgaagccaca cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     3241 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     3301 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     3361 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     3421 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     3481 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     3541 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     3601 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     3661 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     3721 ccgtcatact tgaag
//
