LOCUS       sequence-404887-        2864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT3G08530(H,I)
FEATURES             Location/Qualifiers
     source          1..2864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      288..876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     777..796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1030..1047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1778..1796)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1864..1968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2190..2864,1..117)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
       61 ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg
      121 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
      181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
      241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      961 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1021 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1081 ctccccgcgc gttggccgat tcattaatca ctctgtggtc tcaggagagt gatactctat
     1141 gacataatat tccttcttct tttggatcag attaattaag ttttgtttct aattattaag
     1201 tcatgacact tcttccattc ttaattatta agtttttttt ttcttgtatc aaatctttaa
     1261 ttattaagtt ttctttacgt ataggtttag agtggtgaca cttcttccat tcttaattat
     1321 taagtttttt tcttggatca aatcttaatt attaagtcat ttattgttgt ttcacctaag
     1381 gtaccttcat ttaaaagcac caattaaaat atttcacgtg tcgtctcatt agaaattcgg
     1441 atggaagtca gttgacagag tcgtggatgg gccggacctg gttataaatg taagactcta
     1501 attggatcgc tgttcgtcat tacttaccca tatatatttg ttattcttct tttctcaatt
     1561 ttctttctct ttccatcacc tttctcgttc acttgcttca gatctggcag cgatttcatt
     1621 tcttctccag tctttctctc gtctcggttc tatctcctgg ccattggcgg tcaggacttc
     1681 tcagccgtat atttgattcc gtccccggat ttactcttgc tgtgatctga ggtcaaacaa
     1741 tgtgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta
     1801 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1861 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1921 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc
     1981 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     2041 gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2101 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2161 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     2221 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2281 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc
     2341 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2401 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2461 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2521 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2581 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2641 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2701 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2761 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2821 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat actt
//
