LOCUS       sequence-404891-        2755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G71860
FEATURES             Location/Qualifiers
     source          1..2755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      255..843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     744..763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     997..1014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1636..1654)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1722..1826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2048..2755,1..84)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt aaaaggcgag
       61 atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat actttagatt
      121 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
      181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      361 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      901 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      961 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1021 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc
     1081 tgtggtctca ggaggtgtgt cactcgagac tcggaagtga agagttttta ttattggtta
     1141 tgggctttta agttgggcgt tttaggccca ctgtagtttt ttctgtgacg ttgcccatct
     1201 ttttttttat aatgatgagc ccatctaaaa tgttattcta caaaacaata aaagaaagac
     1261 caggattcct ttgcttaaaa gaccagggtc ctttgctaaa actatattat aatgaccaat
     1321 gaaatcataa ttaataatga ccaattaaaa tgatgacaaa aaaaaaaaaa acccaattaa
     1381 aatagtaaat ttatattata gtcatcaact ttaatacttt gttgcaaaat aaaaggaaac
     1441 agtcgcataa taatccacta atcacaaacc tttacccttt cttctcgggg ccaatcaaaa
     1501 acttcaatta accagaaaag gtcagattct ccgccgcctc acacgacggg caacttcagt
     1561 tcctgccgtc gcgttagatc ccgccgtcaa tcggtcaatg tgagaccacg aagtggctct
     1621 tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1681 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1801 ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga accttgaccg
     1861 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttt
     1921 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     1981 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2041 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2101 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2161 ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga ccgtaaggct
     2221 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     2281 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     2341 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     2401 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     2461 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     2521 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     2581 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     2641 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     2701 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaa
//
