LOCUS       sequence-404892-        2729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT2G33730
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      291..879
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     780..799
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1033..1050
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1646..1664)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1732..1836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2058..2729,1..120)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
       61 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
      121 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      181 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      421 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     1081 cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt gtcgataggc
     1141 tctctggatt tgttcttaac tttcccagtg acgtttacct tttgggattg atctcatgcg
     1201 tgtgcttagt cgcatgtttt atctttaaga ttcgtagttt gatagtgaag gaagcagtag
     1261 caagtgtaat agttgaaact tgagtacctt tttaaaacga tttagtactg gtcttgttca
     1321 agaaaagtat ctttgattct ctgcctaatt tttccacata tatatctcct ctttgtgttt
     1381 taaattgaaa attgatgctc gagacgatgc taagcaagag acaacaatgc aatttggtta
     1441 tgtggaaaat gacttcgcga actcgacctt tcctttatcc taaaacacta agagaatgtg
     1501 aaatattctt ttgtaatatc taatgccccg tggtggattt gtctacaact actatgttgc
     1561 attgttatat gtgggtttgt aacaaataac tcaactactt ttacttcgct tgagaccacg
     1621 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1681 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1741 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1801 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     1861 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1921 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1981 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2041 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2101 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2161 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     2221 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2281 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2341 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2401 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2461 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2521 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2581 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2641 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2701 gcctgccggc ccagtatcag cccgtcata
//
