LOCUS       sequence-404896-        2486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT5G06140
FEATURES             Location/Qualifiers
     source          1..2486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      285..873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     774..793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1027..1044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1397..1415)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1483..1587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1809..2486,1..114)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
       61 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
      121 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
      181 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
      241 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
      301 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
      361 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
      421 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
      481 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
      541 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      601 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      661 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      721 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      781 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      841 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      901 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      961 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1021 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     1081 cccgcgcgtt ggccgattca ttaatcactc tgtggtctca gcttatccaa aactaaactt
     1141 acctgtgtct gtgtgtgttc cttcatgaga gggtttgtgt ctagaggcag tttgtttttg
     1201 tgagaaattc tggtgttttt ttgtcaactt caagtttagt taacttggct tgtttccact
     1261 tgtaaagaag atacatatgc gtcattatta ctctcctaaa cgttcatata acaattttat
     1321 tttagattac aactttccca attcactaac atgataccgc ttgagaccac gaagtggctc
     1381 ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1441 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1501 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1561 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc
     1621 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     1681 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1741 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     1801 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     1861 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     1921 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
     1981 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2041 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2101 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2161 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2221 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2281 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2341 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2401 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2461 cccagtatca gcccgtcata cttgaa
//
