LOCUS       sequence-404898-        2935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT1G54390
FEATURES             Location/Qualifiers
     source          1..2935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      265..853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     754..773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1007..1024
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1826..1844)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1912..2016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2238..2935,1..94)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccattacgt
       61 aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt actcatatat
      121 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
      181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      901 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      961 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1021 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1081 ttaatcactc tgtggtctca gcttgctctc ctcctgcaat ttccatgcat tctgtagcta
     1141 atataatacg tatggagatt tgcacgtact tatgatatta attctcatag gtgccactgg
     1201 catcttcttt ggcaccttcc cttcatgacc aatgccacca aactcatctt tcttgttacc
     1261 caacatctag caagtgcaag tagaagcaaa ctttagatgg agaaatcatc atgttttgta
     1321 aacctcgggt taattcgtgg agcatatatt ttgtatatca attgtatttg ggtattggtg
     1381 ggcattttcc gttttattct actagtatcg atatttacat ctgcttcttt ctttcttttt
     1441 tcccctcaaa ccatgagcct ttgtattgat caaatgacat gtacaaaggt tttgttcatc
     1501 agaaggaatt tcactaaaat aaaaaattgc ttgatatttt attcttgctg tttatatatg
     1561 aaaaaagaga atccattgtg agcagctggt ttgatgtgtc tagatcgata gagggtgtac
     1621 atatatcagt gctagataag ttagttactc ccttagtttg agaagtacta cttgttttga
     1681 ctctattaag gaatactgat attattgtag tttagagagg agagatgctt cacagaacag
     1741 acccacttct gcttcagagg caatgctgtg tttgggacta gtaaaccgct tgagaccacg
     1801 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1861 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1981 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     2041 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     2101 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2161 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2221 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2281 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2341 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     2401 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2461 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2521 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2581 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2641 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2701 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2761 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2821 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2881 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttgg
//
