LOCUS       sequence-404899-        4525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT1G54080
FEATURES             Location/Qualifiers
     source          1..4525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      283..871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     772..791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1025..1042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3434..3452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3520..3624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3846..4525,1..112)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
       61 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
      121 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      181 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      421 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1021 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1081 cgcgcgttgg ccgattcatt aatcactctg tggtctcagg agttaatatc taaattttac
     1141 tcaaatcaaa ttaccaattt tcttcaatgc ggatatacta acatcatgtt atagcctcca
     1201 tgcagagttt aagatgaaca tttttggact gcaagcccat atattttgtt tagccgactt
     1261 tttaaagaat aaattgaaaa acacgaaaga ttgacgttga gattcgaatc gagatgcatg
     1321 attacttaaa accagctaag tttcataaaa actagaccga ttaatatcct ctgaaaaccg
     1381 caaccaaata cttgttcgct tgttctcatg gacgacacta cctccatggt aaaaccttaa
     1441 tagagtcttg atgtaataat tattagcaac aaccatcata actaagacta tctacattgg
     1501 ttacatgatg aatcaaatct ataagaatga catttgtgag tatttcgttt cattttttaa
     1561 agtgtaatta tcactatcac taattttggt ttggataaat gatagatcat aaatctacta
     1621 ttctaagtaa aatctaaaat ggattattgg tatatgtaga ttttaaatat tttattggtt
     1681 tattgattct taaattccat ccaaacttca ttattgttgg ttcaagcttt tttatatatt
     1741 tttattcata tattttgtta ttattgatat tctaattttt tttaaaccta ttgttgttac
     1801 aattctctgt attttggctc tctagttata attgatataa aataacttta aatacttaaa
     1861 aatacaaatc tagaataaga atggagaaat aaatgttatt tatatcaatg agagaagaga
     1921 agaatgcaca aataaagaac attgatatgt tcttttatcc acgtgagaaa aaaagaattc
     1981 attttggagt taaaacattt ccatcaatac atgatttact ttgaaaataa aatgttagga
     2041 aatggttgat aaaattccat ataaactcac tagaaatcat cggtaataat atataagaaa
     2101 aaattataaa ttgtacacaa tcaacttacc aataagtacc atactgtgaa tttgtgaaga
     2161 tatttgatcc tttacaagaa tcttgctgac gtacaaaacg aacgattaaa tataagagat
     2221 aaaccaaaca ataaattata taagaggata atttttataa tatttaaata atctctccta
     2281 tgcagtttca gcttaatcgc gttgtaatct cagcttaact agtatcttac acttgaagat
     2341 caacaaagac aaacctgcaa gatggaaata atcccttgtt aaagtgatga acatatcaat
     2401 atccgcaaca acatcacctt gaagcacatt gcatatcgag ctacaaacaa gagtttggtc
     2461 taggtgagga ttttcatcat ccagatgaat ctttaaaaag aggttcttta tactcgactt
     2521 gattttccga ttaaacaaac acttcacaaa atcaatgaat tccttgatac tatcatgtta
     2581 ttgtatagca taatcaaggt taaaacgccg aaaaagaaca aacacattct ttcactttct
     2641 ggagaatact gtcgttgaaa cagttttttt gtggaaagaa aagataaaat gtatgcaaaa
     2701 acatcgtctt gtaaaaaaaa tcttttaaaa tttaaaattt tctaaatcgt acataatcaa
     2761 cttacaaact tgtgaaaaaa tttggtaata tataacttgt ttgtgaatat atttgatcaa
     2821 atactctaaa aagtactaat aagtttaaaa aaatcttgcc gacaaacaaa acgaacaaac
     2881 ataagggata aaacaaacaa tgaattatgt aagaagataa tgttttttag tgcagtttca
     2941 gtcaatcaaa gaaaatcaaa atttctaagt atatattagt ttgggctggg ccgtcaacaa
     3001 ttggtcagtc actgaaacga atttgttcgg gccttgggct tgatccgtca acaacatcaa
     3061 ggttcagaac aaatgattgg tcagtcacta aaacgaatat cacagctgcc gtgagattag
     3121 tatacagtct atctctctct cgaagaaatc gtcacactcc tataaatggt cacttgtttc
     3181 gtacggcctt tctttactcc tttagagaga gcgtgccatt tttatttttc tctttcttct
     3241 ctcattttta ttttcttttc ttttttttca cctttttttt ttcctttttt tttctttctt
     3301 cttcttctta cttgattttg aaccctagct taaggggaat tttctcggga aacaaaagag
     3361 atatttttat cgcagtgaga aagaaacaca aaaaaatgtg agaccacgaa gtggctcttc
     3421 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     3481 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     3541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3601 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     3661 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
     3721 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3781 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3841 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3901 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3961 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     4021 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     4081 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     4141 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     4201 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     4261 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     4321 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     4381 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     4441 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     4501 agtatcagcc cgtcatactt gaagc
//
