LOCUS       sequence-404901-        3956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT2G26780
FEATURES             Location/Qualifiers
     source          1..3956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      281..869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     770..789
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1023..1040
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2863..2881)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2949..3053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3275..3956,1..110)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
       61 aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc
      121 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
      181 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
      241 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      301 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      361 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      421 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      481 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      541 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      601 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      661 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      721 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      781 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      841 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      901 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      961 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1021 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     1081 cgcgttggcc gattcattaa tcactctgtg gtctcaggag aatttagttt tgtttaaaag
     1141 tttttctggt ttaggtaata aattgttgca taattttcaa ctccggttga tgtgtgtcgc
     1201 tgtcggactg ttgttgtctg aaacttaaaa atcacagagc ggtcacgggt tctgtttcgt
     1261 gtttaactgc cacgatattc tttttaattg ttttttaaaa gggaaagtaa tcaaaatgat
     1321 gacactaata agagtggttt ggtgtgaaac ttagaccttt cttacacaaa tttggtaaag
     1381 ccaaaacttt ttttggtcat ggggatttat ttttttttac tttttatttt tttttttgtc
     1441 ataaagaaat tatagaatct tgttgtgaaa gacgtaaaat ataatgccca aacaaaaaaa
     1501 acgtaaattg gcggataaac tataagttcc caaaaaaaca taagctggcg gaaaaagaga
     1561 attcttcgga atctagatcc taaataaaga agatgaagta aactcaaatt ataatgcaga
     1621 aaatatcaaa ccaatctcga aaaaggacga tagtacaaac taagatccat attaacaaga
     1681 caaaatctta aaaaaataag agatccaaca aagcagaaac tccacttctt ttaacacacc
     1741 tgcaagtacg attgatcaaa tccaaaataa catttaccac ctcctatcac gttgagagcc
     1801 aatctctcga gaaccaagga tatcataacc cgacatgcaa gaccactaaa agaaacacaa
     1861 acaccattcc agatacgacg atgaccacca attcgtcact taacgtctag atagaatcct
     1921 tcaaaaaaca atcagattac gacaaaattg taaaagaagg acaagaagcg aggcaaaact
     1981 tctcaaaacc caccaatcac aagatcacca atcacgagta aagccacaac tattttaatt
     2041 aaatcataca atatcctaaa ttaggctttt ctttttgata aattaaaaaa aaaaagatag
     2101 gatcatccaa caaaaatagt acaaacaaaa aatgtcaaaa tcttgcatca cctctaaaaa
     2161 cctttcatgt taacaaaatc ttgcatattt ttgccctata caaagtcttg catatataca
     2221 aagtcctttg gtatattgtt gttagccacc ttatctaact cttttacggg ctctgtcctc
     2281 atgaagaaga acaatttttt taataaattg ttaccttacc acatataagc ttaatctgga
     2341 atggttgtga tttaatcaat gaagatattg cctcaaacct gaatagagga gtgaaagtgg
     2401 atttcaattg gttggttatc aaacccatca gcatgatacc tgttagtttc tcggccatac
     2461 cactcaatca ttgccataaa tatcattgaa gtctactagt ctgtttgaga cattcaactc
     2521 acacaagtgg tgggttattt tctctgacaa acacggccaa acaataaaat tgctgaaaac
     2581 aagaagttgc ctaaaaaacc aaaggacttg tgagaaaaat ggcaaatgcg ggctaggatg
     2641 ggctgtggat tagaggccca atatgcctaa tgggatacaa cacgacgtcg tttagaccag
     2701 ccatagttct cttgtccgcc aagacgggag agactaagga aaagcataag aagagaagaa
     2761 ccaaagaaga agcaaagaag cactgataaa tctctgcaac tcaacaaatt tctgagatta
     2821 gctaatgtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     2881 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2941 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3001 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     3061 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     3121 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     3181 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     3241 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     3301 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     3361 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gcgatattga
     3421 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     3481 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     3541 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3601 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3661 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3721 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3781 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3841 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3901 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagcta
//
