LOCUS       sequence-404902-        2670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 3'UTR+Terminator part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41276(3Uter)-tAT1G54080
FEATURES             Location/Qualifiers
     source          1..2670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      302..890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     791..810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1044..1061
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1598..1616)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1684..1788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2010..2670,1..131)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
       61 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
      121 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      181 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      421 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      781 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      841 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      901 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      961 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1021 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcagct
     1141 ttaaaccctc ttcactggct ctgagatacc tttttctgtt tctttctttt tcttcttctt
     1201 aattttataa ctttcttgct ttttctagac cttccttgtt caagagtctt tatgtatgtg
     1261 tctctttcat ttaaagccgt tggttttatt tatgtatgca gagctttatg ctcagtttgt
     1321 aacctatagg tcttacttgg attgtaagcc aagcaataag acaacatcaa ataaaagggg
     1381 atttggtttt ctggggttaa tgttgtttgt ggttctgtaa tgataggttt gaaacaaagt
     1441 aatttgtctt ttataaaagt tttatagttt catttctcat gttagtactt tattttgtcg
     1501 gaaatccagt ttaactaagc atcaagtgtt ctgtccacga gtgtcctttg ccatgcagcg
     1561 cttgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg cctattttta
     1621 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc
     1801 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     1861 gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     1921 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     1981 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     2041 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2101 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc
     2161 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2221 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2281 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2341 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2401 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2461 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2521 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2581 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2641 gggcaatgga gcgcctgccg gcccagtatc
//
