LOCUS       sequence-404907-        3040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT4G34270
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      280..868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     769..788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1022..1039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1946..1964)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2032..2136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2358..3040,1..109)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
       61 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
      121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     1081 gcgttggccg attcattaat cactctgtgg tctcaggagt tcacaatttt tgtgttttta
     1141 aaaaacctat aaataaatga gtagtgcaca taaattagtt ttgaccaaaa cggctccaac
     1201 tttaaatatt aaaaagatag tgtgtctaat tctatctgat tcacattgac gcacacatgt
     1261 gttaataaaa taaagatacc attatacaat ttctgtttga tacttatata tgtcaatgtg
     1321 tctcagtatt ctactacgat atataatgaa atatgtttat gcattagagt ctagagattc
     1381 atgaggtttc acctttcact gcttcctgat ttttttacca ctttgaatcg ttaatagtgg
     1441 caatgacgac gaatattttg ttcatgaggt gtatcgtgca aattagttgt gaatttttgt
     1501 gattgattca tcttttgatg atttatatgg atattattga gcttgagaga acaagtgggc
     1561 tagtggtgtt ccaccatgtc aggtttcaaa cctggtctag gtcctctaga ggtttattta
     1621 gccaaatcta ctaataatga aaaaagaaac attgaatatt gacctttttt atagtgtgtt
     1681 ccaaaagcat gaaattctag ctatattaca ttattatgaa aaaggcctac gttataaact
     1741 taaagcccat taaggaccaa aatcaaattg accttctttt ttcaaactaa aatgaaacta
     1801 ggccggccca tcaaaagtca aatgtcttgg ttggatagtc cggttggtca cacaagtgac
     1861 atcgtcggag gagaaaaggt agtgccaata ttagccggcg attcagaatg tgagaccacg
     1921 aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1981 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2041 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2101 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     2161 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     2221 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2281 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2341 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2401 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2461 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     2521 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2581 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2641 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2701 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2761 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2821 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2881 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2941 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3001 gcctgccggc ccagtatcag cccgtcatac ttgaagctag
//
