LOCUS       sequence-404910-        4079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT2G29080
FEATURES             Location/Qualifiers
     source          1..4079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      280..868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     769..788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1022..1039
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2985..3003)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3071..3175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3397..4079,1..109)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
       61 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
      121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
      181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
      241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
      301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
      361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
      421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
      481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
      541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
      601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
      661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
      721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
      781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
      841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
      901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     1081 gcgttggccg attcattaat cactctgtgg tctcaggagt tccaactagc tgttattgct
     1141 atcatgttgt gacagttgcg ttgaaatatt tgcgtttgtg tttggatccg aagcaaacgc
     1201 caaatttgga atttgatatt ctcttttaac ctcttggatc ctaaatgttc gatttatcct
     1261 tttacatttt ctccggcatc attttctaca atgcaataat tccggatatt tgtttgagat
     1321 tccgttcaga catataatga gctgaggtag acgtaattgt ttgtacggaa gcaaaaacgt
     1381 attataatta tttacttgta agtttttagt tggtagtatt agtaagttat acagagttgt
     1441 aaataaacta ataattcgat tagaccacca ttatcaatat tgttgtatgt aacaaacacg
     1501 gaagtacata aatcgttgag ccgtggaaag gagacaaaac atatttttag tctcgtgata
     1561 aaacgagtcg ccacgtgtcc acagtcgggt acgtgcatga gatctaacac actttataac
     1621 tagcttacgt gggactctcg acttccataa aaacgaagaa gattgaaatg aatctaattc
     1681 attgtccccg atttgtctct gtctaaagtc agagaatcag agattcgttt cagagtaaaa
     1741 tgattttgca ttggtccact taagagtctc ttaaccattc caaaggtttg gtggacaaaa
     1801 catgctccaa ccctttctta tccaatttgt cccctcttgt ctctctttaa ttaagcccta
     1861 ccccaaagtt tttcacatga tttgttcttg aaagtgtctc tctcaatctt tgttgtttat
     1921 tattattatt tgatgtgagg cgactagaac gtgtacaaag aaaagagggg agattgggga
     1981 ttaacacgat cgatattcct tttggattag atacgtgaag tacccgaaat gtataagttt
     2041 taacatacac taaattagtg tcagtaataa tcggcgtaaa tgagaggagt ctttcaaaaa
     2101 catacactac atcttatatg tcgatcaata gacatagttc atacccaaaa aaatatacaa
     2161 tacgatggta taaggtgcca tgatttccaa attgagtttt tgcttgatat cagttcctat
     2221 acattgttta tgaggaacaa gttatgtata tatgtctata attttatcat atatatatat
     2281 atatatatga taaaattagt gattatttat ttgtttctca attatgattt aaagagccat
     2341 aattcttctt tagccaaaaa aaaaaaaaaa aagatagaac cataattctt tcattaacaa
     2401 caatgcagat aatttcgttg atgatggtta gtgtcctttt tagttaatat tggttcatta
     2461 ctgaaaataa aataaactga tatgctacgg ctacctcttc acttttattt tcaatttatg
     2521 acaaaagcta tgcccatatg ttctccaagg cccaatatat gtaaaattag gcctatgaac
     2581 caaacactgg tttaataaga ccggtaagca caagttggat tcggacatta tttagaacaa
     2641 aaccggttca agagaatcta cagaacacaa caagtacaga gtctttttgt ctttgacatt
     2701 tcataaacct tctcttctct cgataaactt cagatattaa aaaacccaaa cttttatttc
     2761 ccccaatcaa aaaccctaat ttcttcttat cttgtttgga tttttcattt cttctgtttg
     2821 attttattcc acaaacacaa taaaaaagtt tgcatttttt cctcttctaa gctcgtttca
     2881 gatcttcgat ttcaccattt ttataaacta ttatgcgaac ttagttttgt tctttgtgat
     2941 tttgtaatgt gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct
     3001 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3061 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3121 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg
     3181 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     3241 tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag
     3301 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     3361 cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa
     3421 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     3481 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt
     3541 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     3601 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     3661 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     3721 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     3781 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     3841 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     3901 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     3961 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     4021 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctag
//
