LOCUS       sequence-404914-        3261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo lvl0 Promoter + 5'UTR part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Yang EJY, Nemhauser JL
  TITLE     Building a pipeline to identify and engineer constitutive and 
            repressible promoters.
  JOURNAL   Quant Plant Biol. 2023 Oct 19;4:e12. doi: 10.1017/qpb.2023.10. 
            eCollection 2023.
  PUBMED    37901686
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Quant Plant
            Biol."; date: "2023-10-19"; volume: "4:e12. doi"; pages: " 
            10.1017/qpb.2023.10. eCollection 2023"
COMMENT     Sequence Label: pICH41295(pro5U)-pAT4G16845
FEATURES             Location/Qualifiers
     source          1..3261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      283..871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     772..791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1025..1042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2170..2188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2256..2360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2582..3261,1..112)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
       61 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
      121 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      181 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      241 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      301 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      361 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      421 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      481 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      541 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      601 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      661 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      721 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      781 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      841 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      901 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      961 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1021 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     1081 cgcgcgttgg ccgattcatt aatcactctg tggtctcagg agaatcaaaa tacatattca
     1141 gtaatttatt ttaaactctg actatttatg taaaacacaa atggaaatca aaattgaagg
     1201 tcatgaagat tctattctta gtgtgaaaag tatagatcaa tgattcttaa tttcttcatc
     1261 ctccacgcat agatcaatgg tgaatatggt tttaaatcct ctaatactca ctgtactgcc
     1321 atggtagagt taaaaaaaca attttagaaa tattagtgga ttaaggcatt aagctgtcca
     1381 agttgcttgt attttctttt cattttatta attaaaaaaa aagttcaact atttattgac
     1441 taataataat acgtgttaaa tggttatcgg tttaaaatat gggccatagg cccagacttg
     1501 aagaaaaact tgaaacccaa agttttattt ttacttgttt tctttctcag tgaatatctc
     1561 ccaatcaagc ttcttcaatt ttgcttgctc tctctcttac acggccaatc ggtgttttcg
     1621 cagctttcag gtttgtctca atctcaaatt aaatcggagt caagtaataa caattgataa
     1681 ccctaattgt ttcaattata ttgtaagatt tgaaattttg cagtagatcc ggaatcgtat
     1741 tctagttctg gaatcgttga tctcgatgga atttttttta agatttcttc atacacattt
     1801 ggttcaaaag atcacataat tttattttaa tttgataagt atgatgattc tgctaagtgg
     1861 cattggataa agttttcatt tttgcaatac gtctaaactt gtctatgtct tgaatgaact
     1921 ctctgagttg cttaaaaagt cttgtgcttt ctttattaca caggcctcaa tacaagacat
     1981 tctatataag catattgcag aagaggcggt tctaattgtt gcatggagtt gaacaatatg
     2041 acgtagggaa attctaattt aggggaggcc tcagagtttg cactaacttc ataatcagct
     2101 ctggacgttg ttgattgtat ttgaacaaga aatgtgagac cacgaagtgg ctcttcagtg
     2161 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2221 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2281 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2341 aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2401 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt
     2461 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2521 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2581 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2641 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2701 cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2761 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2821 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2881 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2941 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     3001 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     3061 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3121 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3181 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3241 tcagcccgtc atacttgaag c
//
