LOCUS       sequence-404915-        9932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cytosine base editing using miniSdd3 in monocotyledon plants 
            (transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, 
            Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT,
            Gao C
  TITLE     Discovery of deaminase functions by structure-based protein 
            clustering.
  JOURNAL   Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 
            10.1016/j.cell.2023.05.041. Epub 2023 Jun 27.
  PUBMED    37379837
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.05.041"; journalName: "Cell"; date: 
            "2023-07-20- 20"; volume: "186"; issue: "15"; pages: "3182-3195.e14"
COMMENT     Sequence Label: pnCas9-miniSdd3-PBE
FEATURES             Location/Qualifiers
     source          1..9932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2021..2041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2507..2530,2531..2533,2534..6607)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6608..6655
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6677..6925
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6938..6958
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7032..7208
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7762..7778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7762..7778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7775..7797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7786..7802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7810..7816,7817..7834,7835..7840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7855..7876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7993..8010)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8164..8752)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8244..8263)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8923..9714,9715..9783))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9546..9565
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9784..9888)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg ccaaagaaga agaggaaggt
     2041 tccacggcgc ttcaaccctg cctgctggca gggcctcaag ccatatgatc aagccggcac
     2101 ggcggagggc aacctcttca tcgcgcccgg caagaggtgg aacacaaggc ccatgcaggc
     2161 gtcgaaattg gaagtgggtc cacaatctga cctgcacccg cagtggagaa gcaggaaagc
     2221 gccatggcac attgaaggga agatcgccgc ctacatgagg cagaaggggt ttaccgacgg
     2281 ctgtgtctac cttaatgctc ggccgtgctc aggacctgat ggttgtgcta gaaatctgcc
     2341 ggacctcctg cccgttggta gtacattgca tgttcatgct cgctatattg ataggactgg
     2401 ggagacgcgg ttctactaca gggagtaccg cgggactgga aaagctctta ctaagtccgg
     2461 cagcgagacg ccaggcacct ccgagagcgc tacgcctgaa cttaaggaca agaagtactc
     2521 gatcggcctc gccatcggga cgaactcagt tggctgggcc gtgatcaccg acgagtacaa
     2581 ggtgccctct aagaagttca aggtcctggg gaacaccgac cgccattcca tcaagaagaa
     2641 cctcatcggc gctctcctgt tcgacagcgg ggagaccgct gaggctacga ggctcaagag
     2701 aaccgctagg cgccggtaca cgagaaggaa gaacaggatc tgctacctcc aagagatttt
     2761 ctccaacgag atggccaagg ttgacgattc attcttccac cgcctggagg agtctttcct
     2821 cgtggaggag gataagaagc acgagcggca tcccatcttc ggcaacatcg tggacgaggt
     2881 tgcctaccac gagaagtacc ctacgatcta ccatctgcgg aagaagctcg tggactccac
     2941 cgataaggcg gacctcagac tgatctacct cgctctggcc cacatgatca agttccgcgg
     3001 ccatttcctg atcgaggggg atctcaaccc agacaacagc gatgttgaca agctgttcat
     3061 ccaactcgtg cagacctaca accaactctt cgaggagaac ccgatcaacg cctctggcgt
     3121 ggacgcgaag gctatcctgt ccgcgaggct ctcgaagtcc aggaggctgg agaacctgat
     3181 cgctcagctc ccaggcgaga agaagaacgg cctgttcggg aacctcatcg ctctcagcct
     3241 ggggctcacc ccgaacttca agtcgaactt cgatctcgct gaggacgcca agctgcaact
     3301 ctccaaggac acctacgacg atgacctcga taacctcctg gcccagatcg gcgatcaata
     3361 cgcggacctg ttcctcgctg ccaagaacct gtcggacgcc atcctcctgt cagatatcct
     3421 ccgcgtgaac accgagatca cgaaggctcc actctctgcc tccatgatca agcgctacga
     3481 cgagcaccat caggatctga ccctcctgaa ggcgctggtc cgccaacagc tcccggagaa
     3541 gtacaaggag attttcttcg atcagtcgaa gaacggctac gctgggtaca tcgacggcgg
     3601 ggcctcacaa gaggagttct acaagttcat caagccaatc ctggagaaga tggacggcac
     3661 ggaggagctc ctggtgaagc tcaacaggga ggacctcctg cggaagcaga gaaccttcga
     3721 taacggcagc atcccccacc aaatccatct cggggagctg cacgccatcc tgagaaggca
     3781 agaggacttc taccctttcc tcaaggataa ccgggagaag atcgagaaga tcctgacctt
     3841 cagaatccca tactacgtcg gccctctcgc gcgggggaac tcaagattcg cttggatgac
     3901 ccgcaagtct gaggagacca tcacgccgtg gaacttcgag gaggtggtgg acaagggcgc
     3961 tagcgctcag tcgttcatcg agaggatgac caacttcgac aagaacctgc ccaacgagaa
     4021 ggtgctccct aagcactcgc tcctgtacga gtacttcacc gtctacaacg agctcacgaa
     4081 ggtgaagtac gtcaccgagg gcatgcgcaa gccagcgttc ctgtccgggg agcagaagaa
     4141 ggctatcgtg gacctcctgt tcaagaccaa ccggaaggtc acggttaagc aactcaagga
     4201 ggactacttc aagaagatcg agtgcttcga ttcggtcgag atcagcggcg ttgaggaccg
     4261 cttcaacgcc agcctcggga cctaccacga tctcctgaag atcatcaagg ataaggactt
     4321 cctggacaac gaggagaacg aggatatcct ggaggacatc gtgctgaccc tcacgctgtt
     4381 cgaggacagg gagatgatcg aggagcgcct gaagacgtac gcccatctct tcgatgacaa
     4441 ggtcatgaag caactcaagc gccggagata caccggctgg gggaggctgt cccgcaagct
     4501 catcaacggc atccgggaca agcagtccgg gaagaccatc ctcgacttcc tcaagagcga
     4561 tggcttcgcc aacaggaact tcatgcaact gatccacgat gacagcctca ccttcaagga
     4621 ggatatccaa aaggctcaag tgagcggcca gggggactcg ctgcacgagc atatcgcgaa
     4681 cctcgctggc tcccccgcga tcaagaaggg catcctccag accgtgaagg ttgtggacga
     4741 gctcgtgaag gtcatgggcc ggcacaagcc tgagaacatc gtcatcgaga tggccagaga
     4801 gaaccaaacc acgcagaagg ggcaaaagaa ctctagggag cgcatgaagc gcatcgagga
     4861 gggcatcaag gagctggggt cccaaatcct caaggagcac ccagtggaga acacccaact
     4921 gcagaacgag aagctctacc tgtactacct ccagaacggc agggatatgt acgtggacca
     4981 agagctggat atcaaccgcc tcagcgatta cgacgtcgat catatcgttc cccagtcttt
     5041 cctgaaggat gactccatcg acaacaaggt cctcaccagg tcggacaaga accgcggcaa
     5101 gtcagataac gttccatctg aggaggtcgt taagaagatg aagaactact ggaggcagct
     5161 cctgaacgcc aagctgatca cgcaaaggaa gttcgacaac ctcaccaagg ctgagagagg
     5221 cgggctctca gagctggaca aggccggctt catcaagcgg cagctggtcg agaccagaca
     5281 aatcacgaag cacgttgcgc aaatcctcga ctctcggatg aacacgaagt acgatgagaa
     5341 cgacaagctg atcagggagg ttaaggtgat caccctgaag tctaagctcg tctccgactt
     5401 caggaaggat ttccagttct acaaggttcg cgagatcaac aactaccacc atgcccatga
     5461 cgcttacctc aacgctgtgg tcggcaccgc tctgatcaag aagtacccaa agctggagtc
     5521 cgagttcgtg tacggggact acaaggttta cgatgtgcgc aagatgatcg ccaagtcgga
     5581 gcaagagatc ggcaaggcta ccgccaagta cttcttctac tcaaacatca tgaacttctt
     5641 caagaccgag atcacgctgg ccaacggcga gatccggaag agaccgctca tcgagaccaa
     5701 cggcgagacg ggggagatcg tgtgggacaa gggcagggat ttcgcgaccg tccgcaaggt
     5761 tctctccatg ccccaggtga acatcgtcaa gaagaccgag gtccaaacgg gcgggttctc
     5821 aaaggagtct atcctgccta agcggaacag cgacaagctc atcgccagaa agaaggactg
     5881 ggacccaaag aagtacggcg ggttcgacag ccctaccgtg gcctactcgg tcctggttgt
     5941 ggcgaaggtt gagaagggca agtccaagaa gctcaagagc gtgaaggagc tcctggggat
     6001 caccatcatg gagaggtcca gcttcgagaa gaacccaatc gacttcctgg aggccaaggg
     6061 ctacaaggag gtgaagaagg acctgatcat caagctcccg aagtactctc tcttcgagct
     6121 ggagaacggc aggaagagaa tgctggcttc cgctggcgag ctccagaagg ggaacgagct
     6181 cgcgctgcca agcaagtacg tgaacttcct ctacctggct tcccactacg agaagctcaa
     6241 gggcagcccg gaggacaacg agcaaaagca gctgttcgtc gagcagcaca agcattacct
     6301 cgacgagatc atcgagcaaa tctccgagtt cagcaagcgc gtgatcctcg ccgacgcgaa
     6361 cctggataag gtcctctccg cctacaacaa gcaccgggac aagcccatca gagagcaagc
     6421 ggagaacatc atccatctct tcaccctgac gaacctcggc gctcctgctg ctttcaagta
     6481 cttcgacacc acgatcgatc ggaagagata cacctccacg aaggaggtcc tggacgcgac
     6541 cctcatccac cagtcgatca ccggcctgta cgagacgagg atcgacctct cacaactcgg
     6601 cggggataag agacccgcag caaccaagaa ggcagggcaa gcaaagaaga agaagacgcg
     6661 tgactccggc ggcagcacca acctgtccga catcatcgag aaggagacgg gcaagcaact
     6721 cgtgatccag gagagcatcc tcatgctgcc agaggaggtg gaggaggtca tcggcaacaa
     6781 gccagagtcc gacatcctgg tgcacaccgc ctacgacgag tccaccgacg agaacgtcat
     6841 gctcctgacc agcgacgccc cagagtacaa gccatgggcc ctcgtcatcc aggacagcaa
     6901 cggggagaac aagatcaaga tgctgtcggg ggggagccca aagaagaagc ggaaggtgta
     6961 gtgtacaagt aacccgggaa ttcggtacgc tgaaatcacc agtctctctc tacaaatcta
     7021 tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg
     7081 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     7141 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     7201 tccagatctc ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga cacgagacga
     7261 ctaaacctgg agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt
     7321 agggaaaaga tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat
     7381 atgatgaagt ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg
     7441 caaggtaaga agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag
     7501 ggaatgcttg tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat
     7561 ccgcataagc ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac
     7621 tcaagaggat aaaacctcac caaaatacga aagagttctt aactctaaag ataaaagatc
     7681 tttcaagatc aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg atatctcgag
     7741 atctagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7801 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7861 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7921 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8581 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9061 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9841 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9901 aaaagtgcca cctgccagtg ccaagctaat tc
//
