LOCUS       sequence-404917-       15813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B E210A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15813)
  AUTHORS   Wouters R, Beletchi I, Van den Haute C, Baekelandt V, Martin S, 
            Eggermont J, Vangheluwe P
  TITLE     The lipid flippase ATP10B enables cellular lipid uptake under stress
            conditions.
  JOURNAL   Biochim Biophys Acta Mol Cell Res. 2024 Feb;1871(2):119652. doi: 
            10.1016/j.bbamcr.2023.119652. Epub 2023 Dec 11.
  PUBMED    38086447
REFERENCE   2  (bases 1 to 15813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Cell Res."; date: "2024-02"; volume: 
            "1871(2):119652. doi"; pages: " 10.1016/j.bbamcr.2023.119652. Epub 
            2023 Dec 11"
COMMENT     Sequence Label: pLenti HsATP10B_E210A
FEATURES             Location/Qualifiers
     source          1..15813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1191..1210)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1424..1443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1543..1565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1603..1621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1794..1862,1863..2654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2012..2031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2825..3413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3314..3333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3578..3595
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3665..3685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3670..3999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3850..3985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5133..5198
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5328..5348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5773..5907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5810..5829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5864..5883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6075..6708
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6755..6880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7373..7606
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7791..7835
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8128..8245
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8304..8607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8608..8811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8762..8782
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8808..8832
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8900..8909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9941..9952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    13364..13916
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13508..13525)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13735..13754
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             13916..14518
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     14415..14435
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14538..15126
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14591..14611)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15009..15020)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             15296..15476
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctggc cttttgctca
     3481 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3541 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3601 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3901 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     4021 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     4081 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     4141 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     4201 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     4261 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     4321 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     4381 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     4441 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     4501 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     4561 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     4621 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     4681 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     4741 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     4801 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     4861 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     4921 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     4981 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     5041 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     5101 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     5161 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     5221 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     5281 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     5341 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     5401 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     5461 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     5521 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     5581 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     5641 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     5701 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     5761 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5821 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5881 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     5941 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     6001 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     6061 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     6121 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     6181 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     6241 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     6301 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     6361 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     6421 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     6481 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     6541 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6601 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6661 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6721 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6781 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6841 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6901 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6961 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     7021 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     7081 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     7141 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     7201 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7261 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7321 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7381 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7441 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7501 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7561 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7621 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7681 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7741 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7801 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7861 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7921 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7981 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     8041 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
     8101 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     8161 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     8221 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     8281 gggcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     8341 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     8401 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8461 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     8521 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     8581 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     8641 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     8701 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     8761 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     8821 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     8881 ctctagagga tctgctagcg ccaccatggc cctctcagtg gactcatcgt ggcatcggtg
     8941 gcagtggaga gtcagagatg gcttccccca ttgtccatcg gaaaccacac cgctgctctc
     9001 tccagagaaa gggagacaga gctacaactt gacacagcag cgggtcgtgt tccccaacaa
     9061 cagcatattc catcaagatt gggaagaggt ctccaggaga taccctggca acagaacctg
     9121 cacaaccaaa tacaccctct tcaccttcct gccccggaat ctctttgagc aatttcatag
     9181 atgggctaac ctctatttcc tgttcctggt gattttgaac tggatgccct ccatggaagt
     9241 cttccacaga gaaatcacca tgttaccatt ggccattgtc ctgttcgtca tcatgatcaa
     9301 ggatggcatg gaggacttca agagacaccg ctttgataaa gcaataaact gctccaacat
     9361 tcgaatttat gaaagaaaag agcagaccta tgtgcagaag tgctggaagg atgtgcgcgt
     9421 gggagacttc atccaaatga aatgcaatga gattgtccca gcagacatac tcctcctttt
     9481 ttcctctgac cccaatggga tatgccatct ggaaactgcc agcttggatg gagcgacaaa
     9541 cctcaagcaa agatgtgtcg tgaagggctt ctcacagcag gaggtacagt tcgaaccaga
     9601 gcttttccac aataccatcg tgtgtgagaa acccaacaac cacctcaaca aatttaaggg
     9661 ttatatggag catcctgacc agaccaggac tggctttggc tgtgagagtc ttctgcttcg
     9721 aggctgcacc atcagaaaca ccgagatggc tgttggcatt gtcatctatg caggccatga
     9781 gacgaaagcc atgctgaaca acagtggccc ccggtacaaa cgcagcaaga ttgagcggcg
     9841 catgaatata gacatcttct tctgcattgg gatcctcatc ctcatgtgcc ttattggagc
     9901 tgtaggtcac agcatctgga atgggacctt tgaagaacac cctcccttcg atgtgccaga
     9961 tgccaatggc agcttccttc ccagtgccct tgggggcttc tacatgttcc tcacaatgat
    10021 catcctgctc caggtgctga tccccatctc tttgtatgtc tccattgagc tggtgaagct
    10081 cgggcaagtg ttcttcttga gcaatgacct tgacctgtat gatgaagaga ccgatttatc
    10141 cattcaatgt cgagccctca acatcgcaga ggacttgggc cagatccagt acatcttctc
    10201 cgataagacg gggaccctga cagagaacaa gatggtgttc cgacgttgca ccatcatggg
    10261 cagcgagtat tctcaccaag aaaatgctaa gcgactggag accccaaagg agctggactc
    10321 agatggtgaa gagtggaccc aataccaatg cctgtccttc tcggctagat gggcccagga
    10381 tccagcaact atgagaagcc aaaaaggtgc tcagcctctg aggaggagcc agagtgcccg
    10441 ggtgcccatc cagggccact accggcaaag gtctatgggg caccgtgaaa gctcacagcc
    10501 tcctgtggcc ttcagcagct ccatagaaaa agatgtaact ccagataaaa acctactgac
    10561 caaggttcga gatgctgccc tgtggttgga gaccttgtca gacagcagac ctgccaaggc
    10621 ttccctctcc accacctcct ccattgctga tttcttcctt gccttaacca tctgcaactc
    10681 tgtcatggtg tccacaacca ccgagcccag gcagagggtc accatcaaac cctcaagcaa
    10741 ggctctgggg acgtccctgg agaagattca gcagctcttc cagaagttga agctattgag
    10801 cctcagccag tcattctcat ccactgcacc ctctgacaca gacctcgggg agagcttagg
    10861 ggccaacgtg gccaccacag actcggatga gagagatgat gcatctgtgt gcagtggagg
    10921 tgactccact gatgacggtg gctacaggag cagcatgtgg gaccagggcg acatcctgga
    10981 gtctgggtca ggcacttcct tggaggaggc attggaggcc ccagccacag acctggccag
    11041 gcctgagttc tgttacgagg ctgagagccc tgatgaggcc gccctggtgc acgctgccca
    11101 tgcctacagc ttcacactag tgtcccggac acctgagcag gtgactgtgc gcctgcccca
    11161 gggcacctgc ctcaccttca gcctcctctg caccctgggc tttgactctg tcaggaagag
    11221 aatgtctgtg gttgtgaggc acccactgac tggcgagatt gttgtctaca ccaagggtgc
    11281 tgactcggtc atcatggacc tgctggaaga cccagcctgc gtacctgaca ttaatatgga
    11341 aaagaagctg agaaaaatcc gagcccggac ccaaaagcat ctagacttgt atgcaagaga
    11401 tggcctgcgc acactatgca ttgccaagaa ggttgtaagc gaagaggact tccggagatg
    11461 ggccagtttc cggcgtgagg ctgaggcatc cctcgacaac cgagatgagc ttctcatgga
    11521 aactgcacag catctggaga atcaactcac cttacttgga gccactggga tcgaagaccg
    11581 gctgcaggaa ggagttccag atacgattgc cactctgcgg gaggctggga tccagctctg
    11641 ggtcctgact ggagataagc aggagacagc ggtcaacatt gcccattcct gcagactgtt
    11701 aaatcagacc gacactgttt ataccatcaa tacagagaat caggagacct gtgaatccat
    11761 cctcaattgt gcattggaag agctaaagca atttcgtgaa ctacagaagc cagaccgcaa
    11821 gctctttgga ttccgcttac cttccaagac accatccatc acctcagaag ctgtggttcc
    11881 agaagctgga ttggtcatcg atgggaagac attgaatgcc atcttccagg gaaagctaga
    11941 gaagaagttt ctggaattga cccagtattg tcggtccgtc ctgtgctgcc gctccacgcc
    12001 actccagaag agtatgatag tcaagctggt gcgagacaag ttgcgcgtca tgaccctttc
    12061 cataggtgat ggagcaaatg atgtaagcat gattcaagct gctgatattg gaattggaat
    12121 atctggacag gaaggcatgc aggctgtcat gtccagcgac tttgccatca cccgctttaa
    12181 gcatctcaag aagttgctgc tcgtgcatgg ccactggtgt tactcgcgcc tggccaggat
    12241 ggtggtgtac tacctctaca agaacgtgtg ctacgtcaac ctgctcttct ggtatcagtt
    12301 cttctgtggt ttctccagct ccaccatgat tgattactgg cagatgatat tcttcaatct
    12361 cttctttacc tccttgcctc ctcttgtctt tggagtcctt gacaaagaca tctctgcaga
    12421 aacactcctg gcattgcctg agctatacaa gagtggccag aactctgagt gctataacct
    12481 gtcgactttc tggatttcta tggtggatgc attctaccag agcctcatct gtttctttat
    12541 cccttacctg gcctataagg gctctgatat agatgtcttt acctttggga caccaatcaa
    12601 caccatctcc ctcaccacaa tccttttgca ccaggcaatg gaaatgaaga catggaccat
    12661 tttccacgga gtcgtgctcc tcggcagctt cctgatgtac tttctggtat ccctcctgta
    12721 caatgccacc tgcgtcatct gcaacagccc caccaatccc tattgggtga tggaaggcca
    12781 gctctcaaac cccactttct acctcgtctg ctttctcaca ccagttgttg ctcttctccc
    12841 aagatacttt ttcctgtctc tgcaaggaac ttgtgggaag tctctaatct caaaagctca
    12901 gaaaattgac aaactccccc cagacaaaag aaacctggaa atccagagtt ggagaagcag
    12961 acagaggcct gcccctgtcc ccgaagtggc tcgaccaact caccacccag tgtcatctat
    13021 cacaggacag gacttcagtg ccagcacccc aaagagctct aaccctccca agaggaagca
    13081 tgtggaagag tcagtactcc acgaacagag atgtggcacg gagtgcatga gggatgactc
    13141 atgctcaggg gactcctcag ctcaactctc atccggggag cacctgctgg gacctaacag
    13201 gataatggcc tactcaagag gacagactga tatgtgccgg tgctcaaaga ggagcagcca
    13261 tcgccgatcc cagagttcac tgaccatata gtacctcgag cactagtccc cgggaccgcg
    13321 gcacgcgtgc tctagcccaa ttccgccccc tccccccccc ctaacgttac tggccgaagc
    13381 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
    13441 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
    13501 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
    13561 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
    13621 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
    13681 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
    13741 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
    13801 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
    13861 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggg
    13921 caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
    13981 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
    14041 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    14101 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    14161 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    14221 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    14281 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    14341 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    14401 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    14461 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg
    14521 cgcgttgaat tcccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
    14581 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
    14641 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
    14701 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
    14761 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
    14821 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
    14881 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
    14941 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    15001 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    15061 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
    15121 ccccgcatcg ggaattctgt cgactggtac ctttaagacc aatgacttac aaggcagctg
    15181 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    15241 gaagacaaaa tcgtcgagag atgctgcata taagcagctg ctttttgctt gtactgggtc
    15301 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    15361 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    15421 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
    15481 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
    15541 tgagaggcct tgacattata atagatttag caggaattga actaggagtg gagcacacag
    15601 gcaaagctgc agaagtactt ggaagaagcc accagagata ctcacgattc tgcacatacc
    15661 tggctaatcc cagatcctaa ggattacatt aagtttacta acatttatat aatgatttat
    15721 agtttaaagt ataaacttat ctaatttact attctgacag atattaatta atcctcaaat
    15781 atcataagag atgattacta ttatccccat tta
//
