LOCUS       sequence-404920-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cytosine base editing using miniSdd7 in monocotyledon plants 
            (transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, 
            Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT,
            Gao C
  TITLE     Discovery of deaminase functions by structure-based protein 
            clustering.
  JOURNAL   Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 
            10.1016/j.cell.2023.05.041. Epub 2023 Jun 27.
  PUBMED    37379837
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.05.041"; journalName: "Cell"; date: 
            "2023-07-20- 20"; volume: "186"; issue: "15"; pages: "3182-3195.e14"
COMMENT     Sequence Label: pnCas9-miniSdd7-PBE
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2018..2038
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2564..2587,2588..2590,2591..6664)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6665..6712
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6734..6982
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6995..7015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7089..7265
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7819..7835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7819..7835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7832..7854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7843..7859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7867..7873,7874..7891,7892..7897))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7912..7933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8050..8067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8221..8809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8301..8320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8980..9771,9772..9840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9603..9622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9841..9945)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cctctctacc
     1021 ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt
     1081 ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac
     1141 ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg
     1201 gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat
     1261 agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc
     1321 atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc
     1381 tagatcggag tagaattaat tctgtttcaa actacctggt ggatttatta attttggatc
     1441 tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg
     1501 atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt
     1561 tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg
     1621 agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg
     1681 tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat
     1741 acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat
     1801 atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat aattattttg
     1861 atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg
     1921 ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt
     1981 tggtgttact tctgcaaagc ttgtcgacgg atccatgcca aagaagaaga ggaaggttga
     2041 gggaggaggg cctggggcgg ttcctgaagg aggcgacggc ccgccagccg tgccggcgga
     2101 ggaggtggag aggttgagag gagaacttcc tccacctgtt gtcccgggca ctggtcaaaa
     2161 aacgcatggg cgctggattg gtccagatgg tcgcgtccgc gccattgttt caggaaggga
     2221 tgaagatgct gcactggtgc atgcacagct cgccgccaag gggataccag acgagccaac
     2281 aagaaattct gatgttgagc agaagctggc cgcgcacatg gtggcaaatg gcatccgcca
     2341 cgtcacgctc gtcatcaacc atagaccctg ccgcggcttc gacgacagct gtgatacact
     2401 tgtgcccata attctgccgg agggctgcac cctcacggtg cacgggcaaa ctgacaaggg
     2461 catgagggtg cgggtaaggt acaccggcgg cgcgcggccg tggtggtcca agtccggcag
     2521 cgagacgcca ggcacctccg agagcgctac gcctgaactt aaggacaaga agtactcgat
     2581 cggcctcgcc atcgggacga actcagttgg ctgggccgtg atcaccgacg agtacaaggt
     2641 gccctctaag aagttcaagg tcctggggaa caccgaccgc cattccatca agaagaacct
     2701 catcggcgct ctcctgttcg acagcgggga gaccgctgag gctacgaggc tcaagagaac
     2761 cgctaggcgc cggtacacga gaaggaagaa caggatctgc tacctccaag agattttctc
     2821 caacgagatg gccaaggttg acgattcatt cttccaccgc ctggaggagt ctttcctcgt
     2881 ggaggaggat aagaagcacg agcggcatcc catcttcggc aacatcgtgg acgaggttgc
     2941 ctaccacgag aagtacccta cgatctacca tctgcggaag aagctcgtgg actccaccga
     3001 taaggcggac ctcagactga tctacctcgc tctggcccac atgatcaagt tccgcggcca
     3061 tttcctgatc gagggggatc tcaacccaga caacagcgat gttgacaagc tgttcatcca
     3121 actcgtgcag acctacaacc aactcttcga ggagaacccg atcaacgcct ctggcgtgga
     3181 cgcgaaggct atcctgtccg cgaggctctc gaagtccagg aggctggaga acctgatcgc
     3241 tcagctccca ggcgagaaga agaacggcct gttcgggaac ctcatcgctc tcagcctggg
     3301 gctcaccccg aacttcaagt cgaacttcga tctcgctgag gacgccaagc tgcaactctc
     3361 caaggacacc tacgacgatg acctcgataa cctcctggcc cagatcggcg atcaatacgc
     3421 ggacctgttc ctcgctgcca agaacctgtc ggacgccatc ctcctgtcag atatcctccg
     3481 cgtgaacacc gagatcacga aggctccact ctctgcctcc atgatcaagc gctacgacga
     3541 gcaccatcag gatctgaccc tcctgaaggc gctggtccgc caacagctcc cggagaagta
     3601 caaggagatt ttcttcgatc agtcgaagaa cggctacgct gggtacatcg acggcggggc
     3661 ctcacaagag gagttctaca agttcatcaa gccaatcctg gagaagatgg acggcacgga
     3721 ggagctcctg gtgaagctca acagggagga cctcctgcgg aagcagagaa ccttcgataa
     3781 cggcagcatc ccccaccaaa tccatctcgg ggagctgcac gccatcctga gaaggcaaga
     3841 ggacttctac cctttcctca aggataaccg ggagaagatc gagaagatcc tgaccttcag
     3901 aatcccatac tacgtcggcc ctctcgcgcg ggggaactca agattcgctt ggatgacccg
     3961 caagtctgag gagaccatca cgccgtggaa cttcgaggag gtggtggaca agggcgctag
     4021 cgctcagtcg ttcatcgaga ggatgaccaa cttcgacaag aacctgccca acgagaaggt
     4081 gctccctaag cactcgctcc tgtacgagta cttcaccgtc tacaacgagc tcacgaaggt
     4141 gaagtacgtc accgagggca tgcgcaagcc agcgttcctg tccggggagc agaagaaggc
     4201 tatcgtggac ctcctgttca agaccaaccg gaaggtcacg gttaagcaac tcaaggagga
     4261 ctacttcaag aagatcgagt gcttcgattc ggtcgagatc agcggcgttg aggaccgctt
     4321 caacgccagc ctcgggacct accacgatct cctgaagatc atcaaggata aggacttcct
     4381 ggacaacgag gagaacgagg atatcctgga ggacatcgtg ctgaccctca cgctgttcga
     4441 ggacagggag atgatcgagg agcgcctgaa gacgtacgcc catctcttcg atgacaaggt
     4501 catgaagcaa ctcaagcgcc ggagatacac cggctggggg aggctgtccc gcaagctcat
     4561 caacggcatc cgggacaagc agtccgggaa gaccatcctc gacttcctca agagcgatgg
     4621 cttcgccaac aggaacttca tgcaactgat ccacgatgac agcctcacct tcaaggagga
     4681 tatccaaaag gctcaagtga gcggccaggg ggactcgctg cacgagcata tcgcgaacct
     4741 cgctggctcc cccgcgatca agaagggcat cctccagacc gtgaaggttg tggacgagct
     4801 cgtgaaggtc atgggccggc acaagcctga gaacatcgtc atcgagatgg ccagagagaa
     4861 ccaaaccacg cagaaggggc aaaagaactc tagggagcgc atgaagcgca tcgaggaggg
     4921 catcaaggag ctggggtccc aaatcctcaa ggagcaccca gtggagaaca cccaactgca
     4981 gaacgagaag ctctacctgt actacctcca gaacggcagg gatatgtacg tggaccaaga
     5041 gctggatatc aaccgcctca gcgattacga cgtcgatcat atcgttcccc agtctttcct
     5101 gaaggatgac tccatcgaca acaaggtcct caccaggtcg gacaagaacc gcggcaagtc
     5161 agataacgtt ccatctgagg aggtcgttaa gaagatgaag aactactgga ggcagctcct
     5221 gaacgccaag ctgatcacgc aaaggaagtt cgacaacctc accaaggctg agagaggcgg
     5281 gctctcagag ctggacaagg ccggcttcat caagcggcag ctggtcgaga ccagacaaat
     5341 cacgaagcac gttgcgcaaa tcctcgactc tcggatgaac acgaagtacg atgagaacga
     5401 caagctgatc agggaggtta aggtgatcac cctgaagtct aagctcgtct ccgacttcag
     5461 gaaggatttc cagttctaca aggttcgcga gatcaacaac taccaccatg cccatgacgc
     5521 ttacctcaac gctgtggtcg gcaccgctct gatcaagaag tacccaaagc tggagtccga
     5581 gttcgtgtac ggggactaca aggtttacga tgtgcgcaag atgatcgcca agtcggagca
     5641 agagatcggc aaggctaccg ccaagtactt cttctactca aacatcatga acttcttcaa
     5701 gaccgagatc acgctggcca acggcgagat ccggaagaga ccgctcatcg agaccaacgg
     5761 cgagacgggg gagatcgtgt gggacaaggg cagggatttc gcgaccgtcc gcaaggttct
     5821 ctccatgccc caggtgaaca tcgtcaagaa gaccgaggtc caaacgggcg ggttctcaaa
     5881 ggagtctatc ctgcctaagc ggaacagcga caagctcatc gccagaaaga aggactggga
     5941 cccaaagaag tacggcgggt tcgacagccc taccgtggcc tactcggtcc tggttgtggc
     6001 gaaggttgag aagggcaagt ccaagaagct caagagcgtg aaggagctcc tggggatcac
     6061 catcatggag aggtccagct tcgagaagaa cccaatcgac ttcctggagg ccaagggcta
     6121 caaggaggtg aagaaggacc tgatcatcaa gctcccgaag tactctctct tcgagctgga
     6181 gaacggcagg aagagaatgc tggcttccgc tggcgagctc cagaagggga acgagctcgc
     6241 gctgccaagc aagtacgtga acttcctcta cctggcttcc cactacgaga agctcaaggg
     6301 cagcccggag gacaacgagc aaaagcagct gttcgtcgag cagcacaagc attacctcga
     6361 cgagatcatc gagcaaatct ccgagttcag caagcgcgtg atcctcgccg acgcgaacct
     6421 ggataaggtc ctctccgcct acaacaagca ccgggacaag cccatcagag agcaagcgga
     6481 gaacatcatc catctcttca ccctgacgaa cctcggcgct cctgctgctt tcaagtactt
     6541 cgacaccacg atcgatcgga agagatacac ctccacgaag gaggtcctgg acgcgaccct
     6601 catccaccag tcgatcaccg gcctgtacga gacgaggatc gacctctcac aactcggcgg
     6661 ggataagaga cccgcagcaa ccaagaaggc agggcaagca aagaagaaga agacgcgtga
     6721 ctccggcggc agcaccaacc tgtccgacat catcgagaag gagacgggca agcaactcgt
     6781 gatccaggag agcatcctca tgctgccaga ggaggtggag gaggtcatcg gcaacaagcc
     6841 agagtccgac atcctggtgc acaccgccta cgacgagtcc accgacgaga acgtcatgct
     6901 cctgaccagc gacgccccag agtacaagcc atgggccctc gtcatccagg acagcaacgg
     6961 ggagaacaag atcaagatgc tgtcgggggg gagcccaaag aagaagcgga aggtgtagtg
     7021 tacaagtaac ccgggaattc ggtacgctga aatcaccagt ctctctctac aaatctatct
     7081 ctctctattt tctccataaa taatgtgtga gtagtttccc gataagggaa attagggttc
     7141 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     7201 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     7261 agatctccta aagtccctat agatctttgt cgtgaatata aaccagacac gagacgacta
     7321 aacctggagc ccagacgccg ttcgaagcta gaagtaccgc ttaggcagga ggccgttagg
     7381 gaaaagatgc taaggcaggg ttggttacgt tgactccccc gtaggtttgg tttaaatatg
     7441 atgaagtgga cggaaggaag gaggaagaca aggaaggata aggttgcagg ccctgtgcaa
     7501 ggtaagaaga tggaaatttg atagaggtac gctactatac ttatactata cgctaaggga
     7561 atgcttgtat ttatacccta taccccctaa taacccctta tcaatttaag aaataatccg
     7621 cataagcccc cgcttaaaaa ttggtatcag agccatgaat aggtctatga ccaaaactca
     7681 agaggataaa acctcaccaa aatacgaaag agttcttaac tctaaagata aaagatcttt
     7741 caagatcaaa actagttccc tcacaccggt gacggggatc gcatgcgata tctcgagatc
     7801 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7861 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7921 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7981 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8041 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8101 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8161 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8221 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8281 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8341 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8401 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8461 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8521 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8581 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8641 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8701 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8761 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8821 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8881 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8941 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9001 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9061 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9121 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9181 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9241 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9301 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9361 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9421 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9481 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9541 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9601 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9661 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9721 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9781 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9841 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9901 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9961 agtgccacct gccagtgcca agctaattc
//
