LOCUS       sequence-404925-        2735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ADH2 terminator from yeast (MoClo 3´U +Ter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Fuhrmeister R, Streubel J
  TITLE     Functional Analysis of Plant Monosaccharide Transporters Using a 
            Simple Growth Complementation Assay in Yeast.
  JOURNAL   Bio Protoc. 2023 Aug 5;13(15):e4733. doi: 10.21769/BioProtoc.4733. 
            eCollection 2023 Aug 5.
  PUBMED    37575400
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2023-08-5"; volume: "13(15):e4733. doi"; pages: " 
            10.21769/BioProtoc.4733. eCollection 2023 Aug 5"
COMMENT     Sequence Label: pJS237
FEATURES             Location/Qualifiers
     source          1..2735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(942..1475)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFEIDPSTAT"
     promoter        complement(1476..1580)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1648..1666
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1704..1726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1826..1845
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2039..2061
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2053..2070
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2054..2070
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2257..2444
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2312..2336)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2516..2532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2516..2532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2529..2551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2540..2556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2564..2570,2571..2588,2589..2594))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2609..2630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 acttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaggtggc ggtacttggg
      961 tcgatctcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     1021 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     1081 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc tccggagact
     1141 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     1201 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     1261 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     1321 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     1381 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     1441 ccctgctgcg taacatcgtt gctgctgcgt aacatactct tcctttttca atattattga
     1501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     1621 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     1681 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1741 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1801 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     1861 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     1921 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     1981 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     2041 cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacctc gcgaatgcat
     2101 ctagatttgg tctctgctta agctttggac ttcttcgcca gaggtttggt caagtctcca
     2161 atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa
     2221 atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt
     2281 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     2341 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     2401 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcccgct tgagaccaat
     2461 cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa tcatggtcat
     2521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2701 aacgcgcggg gagaggcggt ttgcgtattg ggcgc
//
