LOCUS       sequence-404926-       11371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 MoClo plasmid for A. baumannii with additional Tellurite 
            resistance cassette for multi-drug resistance strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB2b-TelR
FEATURES             Location/Qualifiers
     source          1..11371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1814..1871
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1895..1912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2066..2654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2146..2165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2837..2931)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             3035..3829
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      3847..3933
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3950..4609)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4524..4543
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     4771..4789
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4793..4836)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    4883..4930
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      4994..5025
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     RBS             7926..7948
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    8040..8087
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    8262..8283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8298..8303,8304..8321,8322..8328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8336..8352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8370..8381
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8388..9065
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      9110..9181
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9197..9224
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttcttaatat gttccacgca agttttagct atggtgctaa acagaaattt gctgaaaaag
       61 aacttttcac tgaactggtt aaaatgtaag cagcctgaga gccgccaaaa attttaaaaa
      121 caaaccgcct taatcatctt caaaaaatac ctctaaaacc tcaccatttg cgttttaaga
      181 cccatatttc atcctgccct tatgttccca tgctgatagc tataaagtgt ctgtaatcgc
      241 ttcctatgac gttctaggct gttgataact tttggaacaa cgcaaaatgt taaaatccga
      301 tcatttttta acctagttat tttcgttaca gggtaacttt ggtagtatta tttcaatatt
      361 tagttgttac aggataacaa aatatatgtt acagggtaat taatttagcg ttacaggata
      421 actatagtta cagggtaacg ttaaatagtt atcctgtaat ttaaaaatag ttgcaggata
      481 gcataaattt ttacaaataa attttataaa aagttatcct gtaactaaaa acaatgttat
      541 cctgtaacta tgatatacta tatgggagtt aaagacatga aagacccgaa cgatcagaaa
      601 acacaggaca tgctaaaaga gccgccaaaa accaatgccg aacgacaaaa agcatatcgt
      661 gaaaaacgca aaagccttga tagtcaacgt ttggaggtgt ttatagataa gggtgtgtcg
      721 gatatgcttg cggacatggt gggcgcagca ggggagagcc aaaaagccat tttgaccgca
      781 ttgattgaga aagagtataa gcggctgtat gcagtgaaaa atagcaagct aaaaaagtac
      841 attgattcaa aaagtaatgc agataaaaaa gaaaccctcg atttatgagg gttttttata
      901 actactaatg cctatgaaaa taagtgctta tcattttctc taatgtaccc aataaaatcg
      961 gggttttgtt ccacaagttt cttgatgtca ttatcatgtt tcttcagtaa gtaagccccg
     1021 atcaggatct tgcgttttgc atcgtctgac cttgcctgtt tttgctctcg acctttcacc
     1081 acagcgtcag ctagatcctt tcttgccttt tcctgacgtt ccaatttttc cttttcttca
     1141 tcgagcattt gacccaattc catagctgtt tttttgacca tttttaatgc ctgtgtctca
     1201 acctattttc cgaagtatga accatcatca atgtgaaaag tgttctactt caaaaactcg
     1261 acttacatca agatccgagc gcagcgagca aaatcaaaaa acaaagtcaa agccttattg
     1321 ctcttgcttt tgcttttagc ctcgcagagt tcccgaaggg cgcacttacg caaaattttt
     1381 gctacgccaa attttgcaag tacggtcagg gaaaccccga caccccaacc gcccaaaact
     1441 tggggcggct gttaataaac aggtgatgaa aatggcaatt taccattgtg aaatgcagaa
     1501 catttcgagg tcagatggtc gctcaatcgt ggcatgtgca gcataccgag caggcgaaaa
     1561 attgtactgt gatacgtacg gaaaagagca ggactacacc aaaaaaacag gcattgaata
     1621 cacccaaatt tttgccccaa ctggggcaag tcctgacatg ttagatcgtc aaaccctatg
     1681 gaatcgggta gagcaatccg aactaaaaaa gaacggtgac atcaaacagg aggcaagatt
     1741 agcaaaggaa gtagagatag cattgccgca tgaactggat aagacacagc gtcaggcaac
     1801 ttgttaactg cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa
     1861 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg
     1921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2041 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
     2101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2821 acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     2881 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     2941 tcgatattct cgagaagctg gggatccgtt tgatttttaa tggataatgt gatataatct
     3001 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     3061 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     3121 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     3181 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     3241 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     3301 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     3361 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     3421 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     3481 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     3541 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     3601 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     3661 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     3721 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     3781 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagg acgtcgccgg
     3841 cggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3901 ttgtcggtga acgctctcct gagtaggaca aatcctcgag aatatcaaat tacgccccgc
     3961 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     4021 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     4081 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     4141 caaaactggt gaaactcacc cagggattgg ctgacacgaa aaacatattc tcaataaacc
     4201 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     4261 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     4321 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     4381 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     4441 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     4501 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     4561 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     4621 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     4681 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
     4741 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     4801 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gccccgggta
     4861 ccgagctcga attggggatc ttgaagtacc tattccgaag ttcctattct ctagaaagta
     4921 taggaacttc agagcgcttt tgaagctgat gtgcttaaaa acttactcaa tggaattagc
     4981 tcctcgagca gaaagtcaaa agcctccgac cggaggcttt tgacttgagg gggatcgatc
     5041 ccttatggct ctgcacccgg ctccatcacc aacaggtcgc gcacgcgctt cactcggttg
     5101 cggatcgaca ctgccagccc aacaaagccg gttgccgccg ccgccaggat cgcgccgatg
     5161 atgccggcca caccggccat cgcccaccag gtcgccgcct tccggttcca ttcctgctgg
     5221 tactgcttcg caatgctgga cctcggctca ccataggctg accgctcgat ggcgtatgcc
     5281 gcttctcccc ttggcgtaaa acccagcgcc gcaggcggca ttgccatgct gcccgccgct
     5341 ttcccgacca cgacgcgcgc accaggcttg cggtccagac cttcggccac ggcgagctgc
     5401 gcaaggacat aatcagccgc cgacttggct ccacgcgcct cgatcagctc ttgcactcgc
     5461 gcgaaatcct tggcctccac ggccgccatg aatcgcgcac gcggcgaagg ctccgcaggg
     5521 ccggcgtcgt gatcgccgcc gagaatgccc ttcaccaagt tcgacgacac gaaaatcatg
     5581 ctgacggcta tcaccatcat gcagacggat cgcacgaacc cgcagaactc acccccgaac
     5641 acgagcacgg cacccgcgac cactatgcca agaatgccca aggtaaaaat tgccggcccc
     5701 gccatgaagt ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc acccaggccg
     5761 ccgccctcac tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca
     5821 atgcttccgg gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc gatcccggca
     5881 atggcaagga ctgccagcgc cgcgatgagg aagcgggtgc cccgcttctt catcttcgcg
     5941 cctcgggcct ccaggccgcc tacctgggcg aaaacatcgg tgtttgtggc attcatacgg
     6001 actcctgttg ggccagctcg cgcacgggct ggcgggtcag cttggcttga agatcgccac
     6061 gcattgcggc gatctgcttc tcggcatcct tgcgcttctg cacgccttcc tgctggatgc
     6121 gaataacgtc ctcgacggtc ttgatgagcg tcgtctgaac ctgcttgagc gtgtccacgt
     6181 cgatcaccag gcgttggttc tccttcgccg tttcgacgga cgtgcgatgc agcagggccg
     6241 cattgcgctt catcaggtcg ttggtggtgt cgtcgatggc cgtggccagt tcgacggcgt
     6301 tcttctgctc gttgaggctc aaggccagca tgaattgccg cttccacgcc ggcacggtga
     6361 tttcgcggat ggtgtggaat ttatcgacca gcatctggtt gttggcctgg atcatgcgga
     6421 tggtcggcag gctctgcatg gccgaatgtt gcaaggcgat caggtcgccg atgcgcttgt
     6481 ccaggttggc aaccatcgca tcgaggtcgg ccagctcctg cacgcggcca gggtcgttcc
     6541 cgacattgcc gcgcagaccc tcggcctgct cgcgcagctc ggcaaggcgg accttgccgg
     6601 ccgcgatgtg gacgccaaga aggcggtgtt cctcgcgcac ggctgcgaac atttcgtcga
     6661 gcgaggcatt gcgctgcgcg atgccttgct gggtggtctg cacttcgctg accaggtgtt
     6721 cgatctgctc gcgggtcgtg tcgaagcgcg ccatgaagcc cgtcgaacgg acgcgggagc
     6781 ggtcgatcag cgggccaatc aggggcaggc gggaacggtt gtcggacaaa gggccgacgt
     6841 tcagggaacg ggccttggcg acaacctggg tcagtttctc gcctgcttcg tccaggtcgc
     6901 tgttgcgcac ctggtccagc aggctatcgg cgtagcggga cgtgtgctcg gccacgtcgc
     6961 ggccgaactc ggcaacggtc tgcggactgc cgacctcgat ccgctgcgcg accgcatgga
     7021 cttccggcac gtcgctttcc tgcaagccca gctcgcgcag ggttgccggg gtcatgtcaa
     7081 aggcgacgat aggggccttg gcgtcgtgcg tcgttttcag tgcgttcata gggttctccc
     7141 gccgtgttat tggttgatgc cttccaggct ctgcgaaagg ctccgcatga gcgcctggtg
     7201 agctttggcc gcctcggcga ccattgccgg attcatgttc ttggtggtga tgagcgcgag
     7261 ggtgtgctga cgccagacgg gcaccaggac ggatgccgtt tcagagaagc ggtccagcat
     7321 gtccacggcc tgcgcccgcg tgagcttcat ctgagtgacg ctcatttcat gggacgccat
     7381 gagggttgcc aggttggcga gcttgcgcgc gaagcgttcg cgcggcttgt cgaactcgat
     7441 cacgccggcc ttggccgcgc cggcctcggg gttctcgtcc aggaactcgc gcccggcttg
     7501 aatgtaggct ctgagccggt ctacctcggc ctcatgcgta ttgagcatgt catccaaggc
     7561 gcgcaacgtg tcccgcacgc gctgcgctac gccctcggct tcgtccagca actggtcgag
     7621 cgtcttgcgg gcgacctgat acctcacctg gcgttcaacc tcacggccaa gcatcttctc
     7681 gaaccaggta ggcttttccg cgatcttgcg ggggtccgcg tcgaccagct tcgccacgat
     7741 ctggctgatt ttgtcggcca gcgcggcaac tgcgccgtgc tccatcagat tcgacagctc
     7801 gttgagggaa tccgccccgt cgatgccggc cccgtactcg ccaatcgtcg ccggcgacgc
     7861 gaagagggcg ggcaaaacct cccccttcaa tcgcgccatg ttcacgcttg ttcttccatg
     7921 gtatatctcc ttcttaaagt taaacaaaat tattcggaac ccagcatgat attccggaaa
     7981 taccaactaa gtcaacggct gatatccatt gctgttgaca aagggaatca ggggatcttg
     8041 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga
     8101 agctaattcg agctcggtac ccgggcggcc gcttctagag gtctcactat atctagagac
     8161 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8221 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8281 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8341 agcggataac aatttcacac atactagaga aagaggagaa atactagatg gcttcctccg
     8401 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
     8461 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
     8521 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
     8581 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     8641 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     8701 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     8761 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     8821 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     8881 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
     8941 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     9001 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     9061 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg
     9121 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     9181 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata cgtctcagaa
     9241 ggtacagaga ccctgcagtt gcaagacaat atcgaccgct tgcagcttga taaagtgatc
     9301 aaagagggac gagcagctac agcactgcta agggagatcg gagagccact gccgccacca
     9361 gtgaaagtga aaacaggttg gtttagtgct gattattacg gctttgagga catgctcaca
     9421 cgttgggata atgatcaatc tagcctttcc atttatcaca gagagataaa agaggagcaa
     9481 caggaacagc aacgaaaaca acagcagata gaaagagaaa ctaaacaaaa acaggaagat
     9541 gacaaaaaga aacggcagga ctatgaaaga tatttatcaa aatacggcta tgtcaaagat
     9601 gctgagtatg accatatcag cgaccatgtt gcttcagaca tttccatgtt tacacgtctt
     9661 caggcgcagg catgggcaag taacgatatg caagagtaca taaggtgttc aaagcaaaaa
     9721 tacgagcaga tcagcgacag aatagcctat gagcgtgact tgaaaaaaat cgggcaattg
     9781 agccgtatcc tggataaaga cagacaggca cttgctgata ttttaccgca gttgatgcct
     9841 tactatgagc agatgcaaca ggctgttaat aaacggaaaa ttttacttga gaatgagcga
     9901 cagccatcat ttaaaccacg ttatgaccaa gaacagccta agccgaaaaa agacaatgac
     9961 ctaacttttt aatgtgttac aatagcattc aaatgtaaca cattggagtg aaaaacatga
    10021 cagaagcgac ttttaccttt cgggtagacc atgacctaaa gcaagaattt tcaagccttg
    10081 ctaagactgt tgatcggtcg ggggcgcagc taatccgtga cttcatgcgt gactttgtta
    10141 agaagcaaca ggaagccgca gactatgaca aatggttcaa gcagcaggta cagatcggct
    10201 taaatgaagc caatgcaggc aagttgattc cacatgagga cgtaaaggca gagtttgccg
    10261 ctagacgtgc tgcaacattg gcgaaactgg cagcgaagaa tgaagattga gtggactgaa
    10321 acagctcgcc aagatcgtag aaatatctat gattatcttg aagaacgcaa ccctatagca
    10381 gctattgaaa ttgatgattt aattgaagaa aagacagatt tacttgttga taatcgactg
    10441 atggggcgca caggcagaca gaaagatact agggagttag tgatacatcc gcattatgtg
    10501 gttgtatatg acatcactga tataatacgg atactcagag tgctacacac atcgcaggag
    10561 tggtcatgac ttactcatgt actttggatt atttagtgtt ataaaatcct gatttataaa
    10621 ttttttttgt taaaaaagat aaaagcccct tgcaattgct tggggcttta ccgtaattta
    10681 tggggtacag atcttcgata ctgacatatc ggcaatcgaa agcattaagg tttgacgacc
    10741 gctaatgatt tcaccacagg ggcttaatgt acctgtctta aattctaagg ttttaactcg
    10801 ctttgtcaag catagacccc aaaaatttag ccaatgtctg taactcaatc tgtccatgtg
    10861 tgggtgatga ggtacagtga cgctagcaca catcggaaaa acgctattac taggggaact
    10921 gaacagagta gcggacgcaa tgagtagtca tttaattggc ggttatgagc gtgttcaggc
    10981 ggtgctatca atcgtaatca taacagtggc agcttgatac agtgatgtca tccctgatgc
    11041 gaaagcgacc gaccgacggt acatcgaatg ggaatacttt agggtgattt ttaagaatcg
    11101 ctctagggtg agtatttccc attcagctct gctccctccc tctggtactt taatcaaaag
    11161 cactactaaa catatgtttt taaataaaaa atattgatat agagataata ttagtaagaa
    11221 taattaaaca attgaatata gataaatcat tgttaaataa agattaatta ttaaaatgaa
    11281 tgtatactta tatataaatc aatgatttaa aatatttgat aaagaaaact tttcaaaaaa
    11341 aatataattg agattgtgtc atttcggtca a
//
