LOCUS       sequence-404928-       10098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pheS_A294G in e.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Park H, Kim S
  TITLE     Gene-specific mutagenesis enables rapid continuous evolution of 
            enzymes in vivo.
  JOURNAL   Nucleic Acids Res. 2021 Apr 6;49(6):e32. doi: 10.1093/nar/gkaa1231.
  PUBMED    33406230
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2021-04-6"; volume: "49(6):e32. doi"; pages: " 
            10.1093/nar/gkaa1231"
COMMENT     Sequence Label: pHyo182
FEATURES             Location/Qualifiers
     source          1..10098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(121..1203)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1165..1184
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1204..1281)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1487..1506
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1590..1609
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1590..1608
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1609..1633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1648..1670
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1689..1706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1716..1733
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2727..2744
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2797..2815)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2811..2858
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3255..3728
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3801..4772)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(4772..5947)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    6273..6523
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(6526..7281)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      7372..7591
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      7678..8313
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8937..9041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9042..9110,9111..9902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9260..9279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cacggaaaaa ggttatgctg cttccaccgg aaggagctga ctgggttgaa ggctctcaag
       61 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
      121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      181 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      241 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      301 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      361 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      421 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      481 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      541 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      601 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      661 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      721 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      781 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      841 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      901 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      961 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     1021 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     1081 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1141 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1201 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1261 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     1321 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     1381 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     1441 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     1501 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     1561 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
     1621 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg
     1681 gcagcagcca tcatcatcat catcacagca gcggcctggt gccgcgcggc agccatatgt
     1741 cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg tcagatgttg
     1801 ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta acccttcaga
     1861 tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg gttatcaacg
     1921 aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg gaaagcgctg
     1981 cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg
     2041 aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg
     2101 agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat aacttcgatg
     2161 ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc tggtttgaca
     2221 ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg aaagcccagc
     2281 agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac gaccagactc
     2341 acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc agctttacca
     2401 acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat ttgcagattc
     2461 gcttccgtcc ttcctacttc ccgtttaccg aaccttctgc ggaagtggac gtcatgggta
     2521 aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac gtgttgcgta
     2581 acgttggcat cgacccggaa gtttactctg gtttcggctt cgggatgggg atggagcgtc
     2641 tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt
     2701 tcctcaaaca gtttaaataa ctcgagcacc accaccacca ccactgagat ccggctgcta
     2761 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     2821 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     2881 gatgaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     2941 taggcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga
     3001 aaacctctga ctcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca
     3061 cggcctgggt aaccaggtat tttgtccaca taaccgtgcg caaaatgttg tggataagca
     3121 ggacacagca gcaatccaca gcaggcatac aaccgcacac cgaggttact ccgttctaca
     3181 ggttacgacg acatgtcaat acttgccctt gacaggcatt gatggaatcg tagtctcacg
     3241 ctgatagtct gatcgacaat acaagtggga ccgtggtccc agaccgataa tcagaccgac
     3301 aacacgagtg ggatcgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt
     3361 ggtcccagac taataatcag accgacgata cgagtgggac cgtggttcca gactaataat
     3421 cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag
     3481 tgggaccatg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag
     3541 tctgattatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga
     3601 cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg
     3661 tggtcccagt ctgattatca gaccgacgat acaagtggaa cagtgggccc agagagaata
     3721 ttcaggccag ttatgctttc tggcctgtaa caaaggacat taagtaaaga cagataaacg
     3781 tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa gttccttaag aatggcctca
     3841 attttctcta tacactcagt tggaacacga gacctgtcca ggttaagcac cattttatcg
     3901 cccttataca atactgtcgc tccaggagca aactgatgtc gtgagcttaa actagttctt
     3961 gatgcagatg acgttttaag cacagaagtt aaaagagtga taacttcttc agcttcaaat
     4021 atcaccccag cttttttctg ctcatgaagg ttagatgcct gctgcttaag taattcctct
     4081 ttatctgtaa aggctttttg aagtgcatca cctgaccggg cagatagttc accggggtga
     4141 gaaaaaagag caacaactga tttaggcaat ttggcggtgt tgatacagcg ggtaataatc
     4201 ttacgtgaaa tattttccgc atcagccagc gcagaaatat ttccagcaaa ttcattctgc
     4261 aatcggcttg cataacgctg accacgttca taagcacttg ttgggcgata atcgttaccc
     4321 aatctggata atgcagccat ctgctcatca tccagctcgc caaccagaac acgataatca
     4381 ctttcggtaa gtgcagcagc tttacgacgg cgactcccat cggcaatttc tatgacacca
     4441 gatactcttc gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga agggatgaga
     4501 tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt cattacctga ccatacccga
     4561 gaggtcttct caacactatc accccggagc acttcaagag taaacttcac atcccgacca
     4621 catacaggca aagtaatggc attaccgcga gccattactc ctacgcgcgc aattaacgaa
     4681 tccaccatcg gggcagctgg tgtcgataac gaagtatctt caaccggttg agtattgagc
     4741 gtatgttttg gaataacagg cgcacgcttc attatctaat ctcccagcgt ggtttaatca
     4801 gacgatcgaa aatttcattg cagacaggtt cccaaataga aagagcattt ctccaggcac
     4861 cagttgaaga gcgttgatca atggcctgtt caaaaacagt tctcatccgg atctgacctt
     4921 taccaacttc atccgtttca cgtacaacat tttttagaac catgcttccc caggcatccc
     4981 gaatttgctc ctccatccac ggggactgag agccattact attgctgtat ttggtaagca
     5041 aaatacgtac atcaggctcg aaccctttaa gatcaacgtt cttgagcaga tcacgaagca
     5101 tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg ggaacaatca
     5161 gcacatcagc agcacatacg acattaatcg tgccgatacc caggttaggc gcgctgtcaa
     5221 taactatgac atcatagtca tgagcaacag tttcaatggc cagtcggagc atcaggtgtg
     5281 gatcggtggg cagtttacct tcatcaaatt tgcccattaa ctcagtttca atacggtgca
     5341 gagccagaca ggaaggaata atgtcaagcc ccggccagca agtgggcttt attgcataag
     5401 tgacatcgtc cttttcccca agatagaaag gcaggagagt gtcttctgca tgaatatgaa
     5461 gatctggtac ccatccgtga tacattgagg ctgttccctg ggggtcgtta ccttccacga
     5521 gcaaaacacg tagccccttc agagccagat cctgagcaag atgaacagaa actgaggttt
     5581 tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg tggaaatacg tcttcagcac
     5641 gtcgcaatcg cgtaccaaac acatcacgca tatgattaat ttgttcaatt gtataaccaa
     5701 cacgttgctc aacccgtcct cgaatttcca tatccgggtg cggtagtcgc cctgctttct
     5761 cggcatctct gatagcctga gaagaaaccc caactaaatc cgctgcttca cctattctcc
     5821 agcgccgggt tattttcctc gcttccgggc tgtcatcatt aaactgtgca atggcgatag
     5881 ccttcgtcat ttcatgacca gcgtttatgc actggttaag tgtttccatg agtttcattc
     5941 tgaacatcct ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt tactgcaaag
     6001 caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata agagcaacac
     6061 tacaaaagga gataagaaga gcacatacct cagtcactta ttatcactag cgctcgccgc
     6121 agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag caaagaagaa ctgttctgtc
     6181 agatagctct tacgctcagc gcaagaagaa atatccaccg tgggaaaaac tccaggtaga
     6241 ggtacacacg cggatagcca attcagagta ataaactgtg ataatcaacc ctcatcaatg
     6301 atgacgaact aacccccgat atcaggtcac atgacgaagg gaaagagaag gaaatcaact
     6361 gtgacaaact gccctcaaat ttggcttcct taaaaattac agttcaaaaa gtatgagaaa
     6421 atccatgcag gctgaaggaa acagcaaaac tgtgacaaat taccctcagt aggtcagaac
     6481 aaatgtgacg aaccaccctc aaatctgtga cagataaccc tcagactatc ctgtcgtcat
     6541 ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt tctttttctc
     6601 aatgtatgag aggcgcattg gagttctgct gttgatctca ttaacacaga cctgcaggaa
     6661 gcggcggcgg aagtcaggca tacgctggta actttgaggc agctggtaac gctctatgat
     6721 ccagtcgatt ttcagagaga cgatgcctga gccatccggc ttacgatact gacacaggga
     6781 ttcgtataaa cgcatggcat acggattggt gatttctttt gtttcactaa gccgaaactg
     6841 cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga tatgggttga tatgtacact
     6901 gtaaagccct ctggatggac tgtgcgcacg tttgataaac caaggaaaag attcatagcc
     6961 tttttcatcg ccggcatcct cttcagggcg ataaaaaacc acttccttcc ccgcgaaact
     7021 cttcaatgcc tgccgtatat ccttactggc ttccgcagag gtcaatccga atatttcagc
     7081 atatttagca acatggatct cgcagatacc gtcatgttcc tgtagggtgc catcagattt
     7141 tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga gactatatgc
     7201 cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt tcttgtgatt
     7261 gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt ttagattgtc
     7321 acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca gcttcttctg
     7381 agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc atttgagggt
     7441 gatttgtcac actgaaaggg caatttgtca caacaccttc tctagaacca gcatggataa
     7501 aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat aattataaaa
     7561 atatccccgt ggataagtgg ataaccccaa gggaagtttt ttcaggcatc gtgtgtaagc
     7621 agaatatata agtgctgttc cctggtgctt cctcgctcac tcgaaattcc cctttccgac
     7681 gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc
     7741 gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cggccgccgg cgttgtggat
     7801 acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg
     7861 ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt
     7921 ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga
     7981 tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta
     8041 ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg
     8101 cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt
     8161 tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta
     8221 taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg
     8281 gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg
     8341 ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg caggggatct cgagggcttc
     8401 gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa aggacagacc acatcatggt
     8461 tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact tcaacgtaac
     8521 accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg ttaccgcttg
     8581 caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg agattaataa
     8641 tgcggatctc tacgataatg ggagattttc ccgactgttt cgttcgcttc tcagtggata
     8701 acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc cacatttcca
     8761 tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac cgcatcagac
     8821 tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa aaccttcgtg
     8881 tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac tttcagcggt
     8941 aaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9001 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9061 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9121 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9181 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9241 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9301 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9361 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9421 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9481 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9541 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9601 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9661 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9721 ggtttattgc tgataaatct ggagccggtg agcgtggttc tcgcggtatc attgcagcac
     9781 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9841 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9901 aactgtcaga ccaagtttac tcaactgcga tgagtggcag ggcggggcgt aattttttta
     9961 aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg
    10021 gatgaatggc agaaattcga tgataagctg tcaaacatga gaattggtcg agcatcttgg
    10081 ttaccgtgaa gttaccat
//
