LOCUS       sequence-404932-       12994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 
            4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic
            domain (Ada) with three repeats of GGGGS linker in between, fol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Sankaranarayanan S, Haag C, Petzsch P, Kohrer K, Matuszynska A, 
            Zarnack K, Feldbrugge M
  TITLE     The mRNA stability factor Khd4 defines a specific mRNA regulon for 
            membrane trafficking in the pathogen Ustilago maydis.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2301731120. doi: 
            10.1073/pnas.2301731120. Epub 2023 Aug 17.
  PUBMED    37590419
REFERENCE   2  (bases 1 to 12994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-08-22"; volume: "120(34):e2301731120. 
            doi"; pages: " 10.1073/pnas.2301731120. Epub 2023 Aug 17"
COMMENT     Sequence Label: p Pcrg1: Khd4-Ada-Gfp (CbxR)
FEATURES             Location/Qualifiers
     source          1..12994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(217..236)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     564..585
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      657..909
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(967..986)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(968..986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1073..1090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1244..1832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1324..1343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2003..2794,2795..2863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2626..2645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4966..4984
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5022..5044)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5144..5163
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5244..5262
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5244..5261
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(12905..12907,12908..12910,12911..12994,1..630)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12905..12907,12908..12910,12911..12994,1..627)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12950..12971)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
       61 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      121 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      181 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      241 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      301 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      361 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      421 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      481 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
      541 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      601 actctcggca tggacgagct gtacaagtaa agcggccggc gcgccggccg cccggcgatc
      661 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
      721 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
      781 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
      841 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
      901 tactagatcc gatgataagc tgtcaaacat gagaattctt atcgatgata tcagatctgc
      961 cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa
     1021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2881 atcgagcacg ttgatgggat cgatcctaga atatcaatcg agccaccgtc aatttcgagc
     2941 gctaagtgga gttgtccgag tcccgaatca caagaattag gctcgtgctc tgtgagatct
     3001 ctcgcgaaac ccagatgaag gaaaaaaatc ggaagatcgc ggaagaagtg gggttcgcat
     3061 ggtctaacat tgtcgcattc ctcacagttt cggctggaaa cggcagggac aatcacgaga
     3121 aatgcgcgac gagtattcag ttgtccaaaa ttatgtcagc ttgaaagttg gaaacggcgg
     3181 aagaaaattc gtcaggaggg cgttgcggct agggtgaaga acggagacaa tttcggcttt
     3241 gaaattggct cgactctgat tggatcatga cgcactgctg gtccacaacc gtgtcgaagg
     3301 tgccgtcttt actacaggtc cgctggaagc aaaatggaaa aagccgctgg agcccgacta
     3361 cagagccgcc gtgttttggt aatcagtcgg caaataggtc agcacagcgc agcgtgacag
     3421 gttcttgcaa tttacagcac agctcgtccg tctacgactt tgcacaccac aaagtgtgcg
     3481 gggagcaaag gagccgatct tggtcgcgcg caaagccaag gagtcttgaa cctgagagtg
     3541 tgcgtgtctt gtgacgcttg cccttctgta ctttgctgtg acactaccac cacatctgtc
     3601 ttggcttttt gttcatacat ccacaccgac catgtcgcta ttcaacgtca gcaacggtct
     3661 tcgtaccgct ctccgacctt ctgttgccag ctcttcgcgc gttgctgcct tttccacaac
     3721 cgccgctgcc cgtctcgcca cacccacctc tgacaacgtt ggcagttcgg gcaagcctca
     3781 gcacttgaag cagttcaaga tctaccgatg gaaccctgac aagccctcgg agaagcctcg
     3841 tctgcagtcg tacacactgg acctcaacca gaccggtcca atggttctcg acgcgctcat
     3901 caaaatcaag aacgaaattg accctacgct caccttccgt cgctcgtgcc gtgagggtat
     3961 ctgcggttcg tgcgctatga atattgacgg tgtcaacacc ctcgcctgcc tctgccggat
     4021 cgacaagcag aatgacacca agatctaccc cttgccgcac atgtacattg tcaaggacct
     4081 cgtgccagac ttgacccagt tctacaagca gtaccgatcc atcgagcctt tcctcaagtc
     4141 caacaacacc ccttctgagg gtgaacatct tcagtcgccc gaggagcgtc gtcgactcga
     4201 cggtctgtac gagtgcattc tgtgcgcgtg ctgctccaca tcctgcccct cttactggtg
     4261 gaatcaggac gagtaccttg gccccgccgt gctcatgcag gcgtaccgat ggatggccga
     4321 ctcgcgtgac gactttggtg aggagcgaag acagaagctc gagaacacct tttcgctcta
     4381 ccgatgcctt accatcatga actgctccag gacctgcccc aagaacctca accctggtaa
     4441 ggcaattgca cagatcaaga aggacatggc cgtcggcgca cccaaggctt ccgagcgccc
     4501 tatcatggct tcgtcgtaat cttgatatat catatcgttc tttcctcagc acttcttttg
     4561 tcaatttcaa aagtatctaa ttgcattcaa ctccgcttgt ggtttgttgt tcagtgagag
     4621 tggaaacgct acgggcaaga tgagggcagt gttctggcga cggaaaagtg tgcaagtgtc
     4681 tggcctgcgt cctcgctggt tccagcagcc gatgcaggac gtgtacctag cgatttcttc
     4741 gacagcctat tgtggcagcc gcgattcgcc acaatcgtac gaaagcgaga cgagttgagc
     4801 gaagatcccc attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4861 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4921 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4981 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     5041 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     5101 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     5161 gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa ttaatacata
     5221 accttatgta tcatacacat acgatttagg tgacactata gaactcgagc agctgaagct
     5281 tgcatgcata gtacatcagg ctactaactg tctttcgcat ctctttgctc aacaagccat
     5341 ccagcgcgca aaagagctcg atgctaagcg tgccctgctc caagagcaag gtcgactgga
     5401 ccagctaggt ccactcttcg gcctgccgat gagtatcaag ggtcaaatgt cctatggaag
     5461 ccacggaaac caacgtggct tcgtctttga cgtcctgccc gaccctgctt cacacccgct
     5521 cgtcaaccgg aatctcactc ggcagcaggt gcagctcttg gcgtcaaccc aaggtggata
     5581 tgtggcagag gagccagtcg attccagcat tgcaaagcta ctactgcaaa acgatgcggt
     5641 catcctggcc aaaacaaccg tgccccaggg cattatgcat ctcgacacga cgagcaactt
     5701 gtacggccaa acgttgaacc cgtgcaatct cgccttgagc ccaggtggtt cgagtggtgg
     5761 agagagtgcg ttggtcgccg gtggcggtag tgcgatgggc gtgggctcgg atatcggtgg
     5821 aagcatccgt cagccgtgcg gcgtgaccgg actatatggc atcaggccta ctacacagag
     5881 gctgccatat atgcaggtat acgctcgacc atgcccggaa atgaggcggt gggatcgtcg
     5941 ttagggccaa tggccaagag tttgagagat ctcgagctgt tcatgtcgag ccttatgaat
     6001 cagaacacta gaccgtggga ctgggaccat accgtgttgc caatgccctg gcgtaaggtg
     6061 gacaccttac tgcagaatgg cgcgtcgcga accaagccta ttattgttgg ggtaatgatg
     6121 gaggacggtg tcgttcgtcc gacgacgcct gtgcgacgtg cgctttgcaa ctgggtcgag
     6181 cagctgaatc ggtttgctag tcaaacagat tctcatctgc cacgcattca acttcgacgt
     6241 tggaacccgc gtgacctgca ccgtcgtgcg tgggacgtga tccgatcact ctactttatg
     6301 gattctggta agatgtttgc tctgctcgcc aaagctactg gcgaaccgct gctcgctctg
     6361 acgcagttca tcctatcgga accctttgtc cgttccttca cggctgatct cgacgacgac
     6421 gctgctcgcg actcgacaat gcgcaaactg gacgaactgt cctactctga gtcgtgcgcc
     6481 aacattcgca tccgcgaggc gttcagaaaa gagtttctgc aagcgtggaa cctgctcgaa
     6541 ctcgactgtc tgctgactcc tgtcatgggc aatgtggcgc ccagaccggc aacagttaca
     6601 tactggggct acacgagcgt tttcaacctg gtcgactatc caggagtggt gttcccgtcc
     6661 ggtttcaagg cggatgccaa ggctgatcag caattcgaag ccgtcgctgc tggtagtggc
     6721 gagtttgcct acaccaacaa gcacgactgg atgagcgagt tcgacaagca caacacgcaa
     6781 gagtatcaac agcacagaca agtgtttgac ggtgctccgg taggcttgca gctgattgga
     6841 cgcaggtaca gggatgaaga actggtaaag tatgccgagc tacttcagca tattgtaggc
     6901 agcaagtgac ggctttctgc gatcctgatg tacatgcaca ctcctttagc tcaaaagcga
     6961 gacactacaa cctgtctcgg atatctcttt gatggcagca tggtgccttg atgtcgccca
     7021 actgttgttt gcgccaagaa cgtccgagca agcagccgtg cacgaagatc tccggtacta
     7081 gagtttcgct tagggatgca aagtgtgaga tttccgagca gtagcgtgga cgcggttttt
     7141 gtcggcatcg gtcaactttt cgagtgatga tgcaacatga agttaggtgt aggcgcgctc
     7201 ttgagcgtgg aagaccatcg cggcatagtg tatgtgtggc ttgaatagcg gcaattccag
     7261 aacgatgctc tggaggctca agacaaagcg gggtggggtg agcgtcgggt ataaagagat
     7321 cacgacaccg cgaggtttgc ggtgaaactc gatgaggcca aaaaagatac cataataggc
     7381 ctgagtggcc atggatttct actcgacgtc cttcactatt ccgcgctcca tcggtgccag
     7441 cagttttgtc acggagacta ccaatcccaa cggcagcatt acagcagcat cgtcgtcgag
     7501 cttcagcctc gaggctcctc gtcctggctc tcatgtaggt gcgcgaactg cgcccacagc
     7561 ttcggctgct cccgagccac ctgcctcttc gttgccaggc cgcttcacct cgatgcgctc
     7621 ccgttttcct cctagcgtca aggctttcat gcccagtgcg tttggcggca agcgcaccag
     7681 cgacaggatc gtcgaggctg tcggcgcaga aaaagcagag gccgacgctg ccaccggctc
     7741 ctcttctcaa cctgttaaag ccgaacccga gcagtcggcg ccgctggccc ctatcggcga
     7801 gaggggaaat gctcgtcctt ccaagctcgt caatcccagt ctggccaaca tggatgacaa
     7861 cggtagcagc cagcatcgtg acagcagatc caccgttggc actcggtcca gctttgacat
     7921 ttcatcggca gcctccttca ccgcagacag caccgctgct agtagcgcca tcggcgtcaa
     7981 ctcgtcgagc accacccatt cggcttccaa ttcgatctcg gccaaccatg tgcagcagga
     8041 tcgaggcatc tcgctttcac agagcagcaa agatcttcaa gaagctatcc agaaactcag
     8101 ctccgatatc atggccaatc accagtgcct agtctcgttt gcttcagtgc cgcaaaacga
     8161 cgagtcgcgc atgcccccaa ccccgctctc gcatcccttt gctcgccaga gctccttctc
     8221 ttcgttgcag agcctcgatc aattgccttt tcccaatcca aatggatctt ccggctatcc
     8281 ctatcagcag taccatccac agcccatcgg cacgcctgac tcgcactcgt ccgccttctt
     8341 ccctggacgc gccgctggag aagcgctcgg ttctggtttc cccggcctca actcaagttc
     8401 gctctcgctc tcgtcgctca atccgcccgc gtctggcagc cacgtcgccc aagaggtcgg
     8461 tctctcgtcc gctcagccgc tccggtcgcc gcagccaaat gccattggtg cttcgcgagc
     8521 cagcctcagt agcatgccca aaccaccgca gtacaacttt catctcagcg gtggctacca
     8581 acaggtcatg aatgctcgag gcaccatctt acgcgaaaat ccgttcaaat cgacctcctc
     8641 catcaaagtg ccgcgcgccg atgtgttcga cgtcgcaccc gcctcgtcgc ctcccgcaaa
     8701 agagagcatc aaggaagatg tgcgtcgcaa actcgatgaa gtacagaccg ccacgggcgc
     8761 aaccatcacg ctcaaaaaca gtgaagtgcg cggcgctgat ctcggctatg gcctcgagac
     8821 cgagcgcatc gtcgaggtgc tcgtctcggg aacctttgaa agtgtcgagt tggcccgggt
     8881 caaggtgctc gtcatcttgg acgagattgc tgggcttcga tccgagacat gcgagatcga
     8941 ctacaagttg cacaacatca tcggcggccg caagcgctgc gttgtgcaga agatcgagga
     9001 agaaacgggc accagcatct acttgccaag ttcttttctc ggcaccttcg gctcgagtct
     9061 tgccagccgc aacgacggca aggcagtcgc ggctcatcaa aatcaaatcc acatcacggg
     9121 cgaattcttt ggcgttcagc gtgcacgcga catgcttttc caggtgtcga tgcacaagag
     9181 caagggcatc atttcacgag atgcagctat cctacctcgc aagctcgatt ggatgctgac
     9241 cgaccgtctc gaggagctcc gatccatcat gatcgacaac ggcacctttg tcgccttccc
     9301 attgctcggc agtcagacca gcgtcatctc ggtctttggc gatcatcgcg ttaacatcga
     9361 acgcacaatc cgatccatca tgcagcttgc gtgccagttc tacgtcgcct cgctctggct
     9421 cttacccgtc ggtttcgacg tctacatgcc atctccacag gctaatctca atccaacgca
     9481 ggtcgcgccc atgctcaagc acgtcgccaa cgcttcgggc gccgaggtgg tgttcaagag
     9541 caactgcttc gagattcacg gtctcgagtc cgaggtgcga accgccgttt cggcgttgct
     9601 cgaactcgag ttgatcaaaa gcttcaactt cgaagtgcgc ttccagattg agctggccaa
     9661 cgagcaccgt gagtttatct cgggcaagaa gaacggcaaa gtcaacaagg tcatgaagca
     9721 gtgtggcgtg cgcatcaagt ttgaaacatt caatgactac aacttcctaa tcgacgtctc
     9781 gggaaacgat cgcaacggag ttttacaagg cctcggtctt ttacaagagg agttgccagc
     9841 cgagatgtcg ttccacgtcc ccgaggcgta ccacaaacgc atcattggcg tcggtggcaa
     9901 gaacatccaa cgcatcatga aaaaatttgg cgtctatgtc aagtttagca atgccgagga
     9961 gtttgctgcg ctcggaggtt acctcgataa cgaagataac gtcatcgcac gaacaccggc
    10021 caaaaatgct gccaaccttg aaaacctcaa gctgtcggtg atggagctgg tcaatcccaa
    10081 ggacaaggac tacatcagtg agacggtcac catctcgcgt cgctaccatc gaactttgct
    10141 tggcgagaag gccatcttca tccatgacat tgagagcaag acaagttcca gcgttcgctt
    10201 ccctgcgcga gagtcagcct cggatctggt gaccatcttc ggacccgagt cgcagattca
    10261 cattgctgcg cagatgcttc tcgaccacgt tccctttgaa gccgagttcc gcgcccccaa
    10321 ctcgaccgag ctcggcgatg ctattcacag tgccgagttt gctgcactcg ctgagcagct
    10381 caagcgcgac ctcagtatct ctgtctcgcc tgtcattgag cagcgacgac ccggtggcga
    10441 ggccgtcttc aagttgcggc tcaacaggag taacaccgac ttcctgccca cggcaaagga
    10501 tgcaatcgag gatttcctca tcaatcgcaa cgccaacatc tacgctgcac cctctcgaac
    10561 tcgctccgat tcgtttgcat cggccttccc gcactttgcc aacaagttga tctcgacggc
    10621 tggcgcggcc gaatcaaatg aatcgttcaa cactgcggcc gcagcggcag ccgccgcggc
    10681 cgaacaggcg cgtgtgaatg agcgcaggct gcgtgctgct gcctcgacac ccgatatcaa
    10741 agcgctcttt gatgcaccgt cccagcacct tcatggcggc ccaggatttg cgtcgagcaa
    10801 tggcggaagc gcagctacag ctgctaactc gcctttgatc acgtctccgc tctacacttc
    10861 gccgtacggt aacggtcgtg ggtttggcag tgatgtgtgg ggcgctccta cacgtgcctt
    10921 cacagccagt aacgctagca acgccacgcc aagcttgatg gggttgcctc cgccttccgt
    10981 cggaacgcct ggcgcaggcc tcgttagtgc agctggacct ggacccatcg gggctggagt
    11041 cagtgtgcca acgagtggcg ggatccagtt tccctcgcag cctagcctgc accagcaaag
    11101 cggacatcgg ctgtcggacg agatgcacat gaaccgaggc ttggagggca tgtcgatgga
    11161 agaccgcgtc aaggcgcttc gaaagcctcg ctcgtttgcg catcgggcac agagtttgga
    11221 cattggcgcc atggcagcgc agcaggcctc gcagcatgca agtggaagca tgagtgttgg
    11281 cccgtcgacg ccttacggtc ttggtcctat cggcacgggt gcccctggca tgtctggcca
    11341 cttccctggc atgacaagct cgcaaggcac ctttggcggt ggtgcacctc accacctcta
    11401 ccagcctcca acaccgcagc agcaggcaca gcagcaactc caataccaac agcagcagca
    11461 gcaacagcag caacagcagc aacagccggg ttacggcatg ccgcatcaac ctcagcactt
    11521 tcatgcgtct tccgctagca tctcgcgcct tccgcccggt cgcaacacac agaaccctga
    11581 ttcgaccacc atggacgagg tgagccgggt gcttgctcaa ctcgcttttg accgggcggc
    11641 caacgcggcc caaggaggcg gtggttccgg tggcggagga tcgggaggtg gcggttcgtc
    11701 catggtggtt cctcaaaaga atgtgccact gcctatcgat gataagtcgt cttctatgga
    11761 actgcctcag atccacgcag ataccattgg ccgactcgtc ctcgaaaagt tcatggaagt
    11821 gattaaggga caggaagcgt attcccgacg caaggtgctg gctggtatcg tcatgaccga
    11881 gaacatgaat ttttgtgagg ctaaggtgat ctctgtctcg accggcacca aatgtgtctc
    11941 cggagagcac atgtcggtca atggagcagt gttgaatgat tcccatgcag aaattgtttc
    12001 ccgtcgttgt ttgctgaaat atctctacgc tcaactggac ctgcaatgca atcaggcaac
    12061 agcttaccaa tcgatcttcg ttcgtaacac agatggacag tatccataca agctcaagag
    12121 cggtgtgcat ttccatctgt acatcaacac agcgccgtgc ggcgacgccc gaattttttc
    12181 gccccacgaa aatgataccg gcgttgataa gcatccaaat cgtaaggcgc gaggccaatt
    12241 gcgaacaaag atcgaatcgg gacaaggcac aatcccggtg aagagcagcg atggcattca
    12301 gacctgggac ggagttctcc aaggacagcg tctgcttacc atgtcgtgct ctgataaaat
    12361 tgctcgctgg aatattgtcg gcatccaggg atcgctgctg tcgtccatca tcgagccggt
    12421 gtacctccat tctatcgttc tgggatcgtt gctgcaccct gagcacatgt accgtgccgt
    12481 gtgtggccgt atcgagaagt cgatccaggg actccctcca ccttaccacc tgaataaacc
    12541 acgacttgct ctcgtcacct cggccgaacc gcgcaatcag gcgaaagcgc ctaactttgg
    12601 cattaactgg accattggag acaccgaact tgaagtggtg aactctctga ctggtcgcac
    12661 cattggcggc caggtttcgc gcatcacgaa gcaagccttt tttgtcaaat atggcttcct
    12721 gatggcgaac ctgcccggta tcctcgtccg taaggttacg acagactatg gccagacgaa
    12781 agcgaacgtg aaagattatc aaattgccaa gctggaactg ttttcggcct ttaaacgcga
    12841 ggatttggga tcgtggctca aaaagccgat cgaacaagat gagttcggac tggcagaatc
    12901 ccatatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
    12961 ggacggcgac gtaaacggcc acaagttcag cgtg
//
