LOCUS       sequence-404944-        8864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under
            the control of a CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, 
            Slupphaug G, Hottiger MO, Schar P, Steinacher R
  TITLE     Covalent PARylation of DNA base excision repair proteins regulates 
            DNA demethylation.
  JOURNAL   Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8.
  PUBMED    38167803
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-01-2"; volume: "15(1):184. doi"; pages: " 
            10.1038/s41467-023-44209-8"
COMMENT     Sequence Label: pCAIP-hXRCC1pd-eGFP
FEATURES             Location/Qualifiers
     source          1..8864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        34..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          654..1669
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1593..1615
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1677..1696
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3700..3709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3706..3708,3709..3711,3712..4425)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3706..3708,3709..3711,3712..4422)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3751..3772)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4012..4031)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4359..4380
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          4503..4732
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4715..4734
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4805..5379,5380..5382,5383..5391)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4972..4989)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5199..5218
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5411..6010
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5411..5430)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5907..5927
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6146..6163)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6152..6376
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6427..6446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6506..6522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6506..6522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6519..6541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6530..6546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6554..6560,6561..6578,6579..6584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6599..6620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6737..6754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6908..7496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6988..7007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7667..8458,8459..8527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8290..8309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8528..8632)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8707..8726)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
      421 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      481 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg cggggcgggg
      541 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      601 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      661 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      721 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      781 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      841 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
      961 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     1021 ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     1081 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
     1141 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
     1201 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     1261 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg
     1321 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     1381 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1441 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     1501 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     1561 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     1621 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     1681 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctagctag cagtgtacag
     1741 atgccggaga tccgcctccg ccatgtcgtg tcctgcagca gccaggactc gactcactgt
     1801 gcagaaaatc ttctcaaggc agacacttac cgaaaatggc gggcagccaa ggcaggcgag
     1861 aagaccatct ctgtggtcct acagttggag aaggaggagc agatacacag tgtggacatt
     1921 gggaatgatg gctcagcttt cgtggaggtg ctggtgggca gttcagctgg aggcgctggg
     1981 gagcaagact atgaggtcct tctggtcacc tcatctttca tgtccccttc cgagagccgc
     2041 agtggcgcta accccaaccg cgttcgcatg tttgggcctg acaagctggt ccgggcagcc
     2101 gccgagaagc gctgggaccg ggtcaaaatt gtttgcagcc agccctacag caaggactcc
     2161 ccctttggct tgagttttgt acggtttcat agccccccag acaaagatga ggcagaggcc
     2221 ccgtcccaga aggtgacagt gaccaagctt ggccagttcc gtgtgaagga ggaggatgag
     2281 agcgccaacg ctctggctcc gggggctctc ttcttcgctc ggatcaacaa gacatcccca
     2341 gtcacagcca gcgacccggc aggacctagc tatgcagctg ctaccctcca ggctgctgcc
     2401 gctgcctcct cagcctctcc agtctccagg gccataggca gcaccgctaa gccccaggag
     2461 tctcccaaag ggaagaggaa gttggatttg aaccaagaag aaaagaagac ccccagcaaa
     2521 ccaccagccc agctgtcgcc agctgttccc aagagaccta aattgccagc tccaactcgt
     2581 accccagcca cagccccagt ccctgcccga gcacaggggg cagtgacagg caaaccccga
     2641 ggagaaggca ccgagcccag acgaccccga gctggcccag aggagctggg gaagatcctt
     2701 cagggtgtgg tagtggtgct gagtggcttc cagaacccct tccgctccga gctgcgagat
     2761 aaggccctag agcttggggc caagtatcgg ccagactgga cccgggacag cacgcacctc
     2821 atctgtgcct ttgccaacac ccccaagtac agccaggtcc taggcctggg aggccgcatc
     2881 gtgcgtaagg agtgggtgct ggactgtcac cgcatgcgtc ggcggctgcc ctcccggagg
     2941 tacctcatgg cagggccagg ttccagcagt gaggaggatg aggcctctca cagcggtggc
     3001 agcggagatg aagcccccaa gcttcctcag aagcaacccc agaccaaaac caagcccact
     3061 caggcagctg gacccagctc accccagaag cccccaaccc ctgaagagac caaagcagcc
     3121 tcaccagtgc tccaggaaga tatagacatt gagggggtac agtcagaagg acaggacaat
     3181 ggggcggaag attctgggga cacagaggat gagctgagga gggtggcaga gcagaaggaa
     3241 cacagactgc cccctggcca ggaggagaat ggggaagacc cgtatgcagg ctccacggat
     3301 gagaacacgg acagtgagga acaccaggag cctcctgatc tgccagtccc tgagctccca
     3361 gatttcttcc agggcaagca cttctttctt tacggggagt tccctgggga cgagcggcgg
     3421 aaactcatcc gatacgtcac agccttcaat ggggagctcg aggactatat gagtgaccgg
     3481 gttcagtttg tgatcacagc acaggaatgg gatcccagct ttgaggaggc cctgatggac
     3541 aacccctccc tggcattcgt tcgtccccga tggatctaca gttgcaatga gaagcagaag
     3601 ttacttcctc accagctcta tggggtggtg ccgcaagccg tcgagctcaa gcttcgaatt
     3661 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc
     3721 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3781 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3841 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3901 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3961 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4021 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4081 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4141 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4201 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4261 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4321 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4381 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgatggatc
     4441 cactagtaac ggccgccagt gtgctggaat taattcgctg tctgcgaggg ccagctgttg
     4501 gggtgagtac tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa
     4561 aaacgaggag gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc
     4621 catctggtca gaaaagacaa tctttttgtt gtcaagcttg aggtgtggca ggcttgagat
     4681 ctggccatac acttgagtga caatgacatc cactttgcct ttctctccac aggtgtccac
     4741 tcccaggtcc aactgcaggt cgagcatgca tctagggcgg ccaattccgc ccctctcccc
     4801 cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4861 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4921 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4981 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5041 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5101 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5161 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5221 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5281 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5341 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag
     5401 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5461 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5521 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5581 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5641 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5701 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5761 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5821 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5881 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5941 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6001 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     6061 tggctccgac cgaagccacc cggggcggcc ccgccgaccc cgcacccgcc cccgaggccc
     6121 accgactcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6181 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6241 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6301 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6361 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat cgagtgcatt
     6421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6481 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8641 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     8701 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     8761 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     8821 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgc
//
