LOCUS       sequence-404945-        8880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hXRCC1wt coupled to eGFP under the control 
            of a CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Schwarz SD, Xu J, Gunasekera K, Schurmann D, Vagbo CB, Ferrari E, 
            Slupphaug G, Hottiger MO, Schar P, Steinacher R
  TITLE     Covalent PARylation of DNA base excision repair proteins regulates 
            DNA demethylation.
  JOURNAL   Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8.
  PUBMED    38167803
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-01-2"; volume: "15(1):184. doi"; pages: " 
            10.1038/s41467-023-44209-8"
COMMENT     Sequence Label: pCAIP-hXRCC1wt-eGFP
FEATURES             Location/Qualifiers
     source          1..8880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        34..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        415..669
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          670..1685
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1609..1631
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1693..1712
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3716..3725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3722..3724,3725..3727,3728..4441)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3722..3724,3725..3727,3728..4438)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3767..3788)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4028..4047)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4375..4396
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          4519..4748
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4731..4750
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4821..5394
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4988..5005)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5215..5234
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5427..6026
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5427..5446)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5923..5943
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6162..6179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6168..6392
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6443..6462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6522..6538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6522..6538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6535..6557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6546..6562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6570..6576,6577..6594,6595..6600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6615..6636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6753..6770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6924..7512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7004..7023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7683..8474,8475..8543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8306..8325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8544..8648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8723..8742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
      421 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      481 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ngggcggggc
      541 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
      601 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
      661 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
      721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
      781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
      841 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
      901 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
      961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1021 gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc
     1081 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc
     1141 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta
     1201 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1261 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga
     1321 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     1381 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     1441 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     1501 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     1561 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     1621 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     1681 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattct
     1741 agctagcagt gtacagatgc cggagatccg cctccgccat gtcgtgtcct gcagcagcca
     1801 ggactcgact cactgtgcag aaaatcttct caaggcagac acttaccgaa aatggcgggc
     1861 agccaaggca ggcgagaaga ccatctctgt ggtcctacag ttggagaagg aggagcagat
     1921 acacagtgtg gacattggga atgatggctc agctttcgtg gaggtgctgg tgggcagttc
     1981 agctggaggc gctggggagc aagactatga ggtccttctg gtcacctcat ctttcatgtc
     2041 cccttccgag agccgcagtg gctcaaaccc caaccgcgtt cgcatgtttg ggcctgacaa
     2101 gctggtccgg gcagccgccg agaagcgctg ggaccgggtc aaaattgttt gcagccagcc
     2161 ctacagcaag gactccccct ttggcttgag ttttgtacgg tttcatagcc ccccagacaa
     2221 agatgaggca gaggccccgt cccagaaggt gacagtgacc aagcttggcc agttccgtgt
     2281 gaaggaggag gatgagagcg ccaactctct gaggccgggg gctctcttct tcagccggat
     2341 caacaagaca tccccagtca cagccagcga cccggcagga cctagctatg cagctgctac
     2401 cctccaggct tctagtgctg cctcctcagc ctctccagtc tccagggcca taggcagcac
     2461 ctccaagccc caggagtctc ccaaagggaa gaggaagttg gatttgaacc aagaagaaaa
     2521 gaagaccccc agcaaaccac cagcccagct gtcgccatct gttcccaaga gacctaaatt
     2581 gccagctcca actcgtaccc cagccacagc cccagtccct gcccgagcac agggggcagt
     2641 gacaggcaaa ccccgaggag aaggcaccga gcccagacga ccccgagctg gcccagagga
     2701 gctggggaag atccttcagg gtgtggtagt ggtgctgagt ggcttccaga accccttccg
     2761 ctccgagctg cgagataagg ccctagagct tggggccaag tatcggccag actggacccg
     2821 ggacagcacg cacctcatct gtgcctttgc caacaccccc aagtacagcc aggtcctagg
     2881 cctgggaggc cgcatcgtgc gtaaggagtg ggtgctggac tgtcaccgca tgcgtcggcg
     2941 gctgccctcc cggaggtacc tcatggcagg gccaggttcc agcagtgagg aggatgaggc
     3001 ctctcacagc ggtggcagcg gagatgaagc ccccaagctt cctcagaagc aaccccagac
     3061 caaaaccaag cccactcagg cagctggacc cagctcaccc cagaagcccc caacccctga
     3121 agagaccaaa gcagcctcac cagtgctcca ggaagatata gacattgagg gggtacagtc
     3181 agaaggacag gacaatgggg cggaagattc tggggacaca gaggatgagc tgaggagggt
     3241 ggcagagcag aaggaacaca gactgccccc tggccaggag gagaatgggg aagacccgta
     3301 tgcaggctcc acggatgaga acacggacag tgaggaacac caggagcctc ctgatctgcc
     3361 agtccctgag ctcccagatt tcttccaggg caagcacttc tttctttacg gggagttccc
     3421 tggggacgag cggcggaaac tcatccgata cgtcacagcc ttcaatgggg agctcgagga
     3481 ctatatgagt gaccgggttc agtttgtgat cacagcacag gaatgggatc ccagctttga
     3541 ggaggccctg atggacaacc cctccctggc attcgttcgt ccccgatgga tctacagttg
     3601 caatgagaag cagaagttac ttcctcacca gctctatggg gtggtgccgc aagccgtcga
     3661 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
     3721 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3781 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3841 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3901 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3961 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     4021 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     4081 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     4141 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     4201 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     4261 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     4321 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     4381 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     4441 aagcggccga tggatccact agtaacggcc gccagtgtgc tggaattaat tcgctgtctg
     4501 cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa
     4561 gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt
     4621 tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt
     4681 gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct
     4741 ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta gggcggccaa
     4801 ttccgcccct ctcccccccc ccccyctccc tccccccccc ctaacgttac tggccgaagc
     4861 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4921 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4981 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     5041 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     5101 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     5161 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     5221 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     5281 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     5341 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     5401 cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca
     5461 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5521 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5581 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5641 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5701 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5761 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     5821 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     5881 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     5941 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6001 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     6061 ggagcgcacg accccatggc tccgaccgaa gccacccggg gcggccccgc cgaccccgca
     6121 cccgcccccg aggcccaccg actctagagc tcgctgatca gcctcgactg tgccttctag
     6181 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6241 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6301 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6361 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6421 ctcgatcgag tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6481 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     7501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     7561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     7621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7801 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     8641 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     8701 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8761 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8821 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
//
