LOCUS       sequence-404960-       11292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium transformation. A plastid transit peptide fused 
            mCherry-UnaG under the control of the cauliflower mosaic virus 
            (CaMV) 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
  TITLE     Bilirubin is produced nonenzymatically in plants to maintain 
            chloroplast redox status.
  JOURNAL   Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. 
            Epub 2023 Jun 7.
  PUBMED    37285441
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-9"; volume: "9(23):eadh4787. doi"; pages: " 
            10.1126/sciadv.adh4787. Epub 2023 Jun 7"
COMMENT     Sequence Label: pGWB602-TP-mCherry-UnaG
FEATURES             Location/Qualifiers
     source          1..11292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1170..1194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1455..2162
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1868..1887
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2193..2612
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
                     KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
                     GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     protein_bind    complement(2623..2647)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2679..2931
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2964..2980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2964..2980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2977..2999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2988..3004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3012..3018,3019..3036,3037..3042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3057..3078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3114..3366)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3526..4077)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4129..4308)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4814..4838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5359..6150
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6396..6984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6885..6904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7138..7155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7170..7310
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7236..7255)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7396..7418
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7654..7848
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7914..8987)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9416..10045)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gagttatcaa caagtttgta caaaaaagca ggctttaaag
     1201 gaaccaattc agtcgacatg gcttcctcta tgctctcttc cgctactatg gttgcctctc
     1261 cggctcaggc cactatggtc gctcctttca acggacttaa gtcctccgct gccttcccag
     1321 ccacccgcaa ggctaacaac gacattactt ccatcacaag caacggcgga agagttaact
     1381 gcatgcaggt gtggcctccg attggaaaga agaagtttga gactctctct taccttcctg
     1441 accttaccga ttccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     1501 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1561 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1621 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     1681 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     1741 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     1801 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     1861 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     1921 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     1981 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2041 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2101 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2161 agggtggttc tggtggatcc ggtggttctg gtatggtcga gaaatttgtt ggcacctgga
     2221 agatcgcaga cagccataat tttggtgaat acctgaaagc tatcggagcc ccaaaggaat
     2281 taagcgatgg tggggatgcc acgacgccga cattgtacat ctcccagaag gacggagaca
     2341 aaatgacagt gaaaatagag aatggacctc ctacgttcct tgacactcaa gtaaagttca
     2401 aattagggga ggagttcgac gaatttcctt ctgatcgaag aaaaggcgta aaatctgtcg
     2461 tgaacttggt gggagagaag ctggtgtacg tacaaaagtg ggacggcaag gagacgacgt
     2521 atgtccgaga gataaaggac ggtaaactgg tcgtgacact tacgatggga gacgtcgtgg
     2581 ctgtgcgcag ctaccggagg gcgacggaat gaggatatct agacccagct ttcttgtaca
     2641 aagtggttga taacagcgct tagagctcga atttccccga tcgttcaaac atttggcaat
     2701 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     2761 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     2821 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     2881 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattgg
     2941 ttccggaacc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3001 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3061 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag
     3121 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     3181 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     3241 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     3301 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     3361 acgatcgggg aaattcgagc tggggacgtc cccagctcgg taccccctcc tgggcacggt
     3421 cgtacgcgat ccgccgcggt tccacctcac cattgcgggt atgccagttg agttcgatga
     3481 gttcccgtac cagctccaac tcgggcgatg ccacgcgcac tccgttcaga tctcggtgac
     3541 gggcaggacc ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc
     3601 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
     3661 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
     3721 gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt
     3781 ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
     3841 tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
     3901 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
     3961 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
     4021 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatatc
     4081 ttattgcccc ccgggatcag atctggattg agagtgaata tgagactcta attggatacc
     4141 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     4201 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     4261 taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa
     4321 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     4381 cgctcatgat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     4441 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     4501 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     4561 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
     4621 gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
     4681 gcgccctgag tgcttgcggc agcgtgaagc tgatccgtcg agattaatag aaataaattc
     4741 agcctaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
     4801 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
     4861 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
     4921 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
     4981 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
     5041 attgtaacga tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca
     5101 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     5161 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     5221 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     5281 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     5341 aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca
     5401 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     5461 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     5521 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     5581 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     5641 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     5701 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     5761 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     5821 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     5881 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     5941 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     6001 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     6061 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     6121 ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg
     6181 ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag
     6241 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
     6301 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga
     6361 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6541 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6781 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6841 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6901 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6961 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7021 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7081 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7141 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     7201 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7261 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     7321 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7381 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7441 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
     7501 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
     7561 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
     7621 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
     7681 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
     7741 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
     7801 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
     7861 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
     7921 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
     7981 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
     8041 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
     8101 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
     8161 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
     8221 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
     8281 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
     8341 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
     8401 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
     8461 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
     8521 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
     8581 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
     8641 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
     8701 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
     8761 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
     8821 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
     8881 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
     8941 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg
     9001 gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc
     9061 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
     9121 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
     9181 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
     9241 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
     9301 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
     9361 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
     9421 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
     9481 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
     9541 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
     9601 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
     9661 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
     9721 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
     9781 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
     9841 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
     9901 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
     9961 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    10021 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    10081 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    10141 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    10201 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    10261 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    10321 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    10381 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    10441 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    10501 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    10561 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    10621 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    10681 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    10741 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    10801 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    10861 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    10921 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    10981 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    11041 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    11101 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    11161 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    11221 atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga
    11281 acggataaac ct
//
