LOCUS       sequence-404962-       14040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana 
            NAD KINASE2 (NADK2) under the control of the cauliflower mosaic 
            virus (CaMV) 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14040)
  AUTHORS   Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
  TITLE     Bilirubin is produced nonenzymatically in plants to maintain 
            chloroplast redox status.
  JOURNAL   Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. 
            Epub 2023 Jun 7.
  PUBMED    37285441
REFERENCE   2  (bases 1 to 14040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-9"; volume: "9(23):eadh4787. doi"; pages: " 
            10.1126/sciadv.adh4787. Epub 2023 Jun 7"
COMMENT     Sequence Label: pGWB560-NADK2
FEATURES             Location/Qualifiers
     source          1..14040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4192..4216)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4227..4940
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      4962..5214
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5247..5263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5247..5263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5260..5282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5271..5287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5295..5301,5302..5319,5320..5325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5340..5361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5397..5649)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5800..6825)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6877..7056)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7562..7586
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8107..8898
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9144..9732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9633..9652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9886..9903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9918..10058
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9984..10003)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10144..10166
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10402..10596
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10662..11735)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12164..12793)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgt tcctatgctt ttgcccttgc cacgtaccta
     1261 tcatgagtcg cctttctccg gccaccggaa tctcttcccg cctccgcttc tccattggtt
     1321 tgagttccga tggacgatta attcccttcg gattccggtt tcggagaaac gatgtcccgt
     1381 ttaaacgccg cttgagattt gtgatcagag cgcagctctc tgaagctttt tctcccgatt
     1441 taggtttgga ttctcaggct gtgaaatccc gcgatacatc aaacttgcct tggattggtc
     1501 cagttccagg ggacattgct gaggttgagg cgtattgtag aatttttaga tcagctgagc
     1561 gactacatgg agcgttgatg gagacactat gcaaccctgt gactggtgaa tgtcgagtac
     1621 cgtatgattt ctcaccggag gaaaaaccat tgttggagga caaaatagta tcagtgcttg
     1681 gttgtatatt atctctttta aacaaaggaa ggaaagaaat tctctctggg aggtcatctt
     1741 ctatgaattc atttaatttg gatgatgttg gggttgcaga ggagtcgctt ccaccacttg
     1801 ctgttttcag gggtgaaatg aaacggtgtt gtgaaagctt acacattgct cttgagaatt
     1861 atctgacgcc ggatgatgag agaagtggaa ttgtttggag gaaattacag aagcttaaaa
     1921 atgtctgcta cgacgctggt tttccacgca gtgataacta tccttgtcaa acactttttg
     1981 cgaattggga ccctatttac tcgtcaaata cgaaagagga tattgattcc tacgagtctg
     2041 agattgcatt ttggagggga ggacaggtaa cccaagaagg attgaagtgg ttgatagaaa
     2101 acggatttaa aacaattgtt gacctgagag ctgaaatcgt taaggataca ttctaccaga
     2161 cggcacttga tgatgcaatt tcccttggga aaattacagt ggtgcaaatt ccaattgatg
     2221 tcaggatggc tcctaaagct gagcaggtcg agctgtttgc ttctattgta tcagatagca
     2281 gcaaaagacc gatatatgtt cacagtaaag aaggtgtttg gagaacttct gcgatggttt
     2341 ctaggtggaa gcagtacatg acacgaccga tcacgaaaga aattccagtt tcagaagagt
     2401 caaagcgtcg ggaggtttct gaaactaagc ttggatcaaa tgctgtagta tctggtaagg
     2461 gtgtacctga tgagcagact gataaagtct ctgaaatcaa tgaggttgat agtagatctg
     2521 cttcaagcca gagcaaggaa tctggaaggt ttgagggaga tacatctgca tcagaattta
     2581 atatggtgag cgatcctctt aaatctcaag ttccaccagg caatattttt tcaagaaaag
     2641 aaatgtctaa attcctgaag agcaaaagta ttgctcctgc tggttatctt actaatccgt
     2701 ccaaaatatt gggaacagtg ccaactccac aattttcata tactggtgtg acaaacggaa
     2761 atcagattgt tgataaagat tcgataagaa gacttgcgga gacaggaaac tccaatggga
     2821 cccttctacc tacaagttct caaagtttag attttggcaa cgggaagttt tcaaatggaa
     2881 atgtgcatgc gtctgataac accaacaaaa gtatatcgga caacagggga aatggcttct
     2941 ctgcagcccc tattgctgtg cctcctagtg ataacttaag tcgcgctgta ggatcccatt
     3001 cggttcgaga gtctcagact cagagaaata atagtggttc ctcctcggat tccagtgatg
     3061 atgaagctgg agctattgag ggaaatatgt gtgcttctgc cactggtgta gtaagggtgc
     3121 agtcgagaaa gaaagcagag atgttcttag tccgaactga tggagtgtct tgtacaaggg
     3181 aaaaggtgac agaatcctct ctggccttca cacatccaag tactcaacag cagatgcttc
     3241 tttggaaaac taccccaaaa actgtcttac tgctgaagaa gctcgggcaa gaactgatgg
     3301 aggaagctaa agaggctgcc tctttcttgt atcaccaaga gaatatgaat gttctggttg
     3361 aacctgaggt gcatgatgta tttgccagga ttccagggtt tggctttgtc cagaccttct
     3421 acattcagga cacgagcgat ctccatgaaa gggttgattt tgtggcatgc ttaggggggg
     3481 atggggtgat attacatgca tcaaacttgt tcaaaggagc cgtccctccc gttgtttcat
     3541 ttaatctggg gtctcttgga tttctcactt cacatccatt tgaggacttc aggcaagacc
     3601 tcaaacgagt catccatggg aataacacgc tagatggggt ttatataact cttcgaatgc
     3661 gtcttcgttg cgaaatctat cgtaaaggca aagcaatgcc tggtaaagtg tttgatgttc
     3721 tgaacgagat tgttgttgat cgaggatcca acccatacct ttctaagatc gaatgttatg
     3781 agcacgaccg tcttatcacg aaggtacaag gcgatggagt tatagtagcc actcctacag
     3841 gaagtactgc ttattctaca gcagcaggag gttccatggt gcatccaaac gttccttgca
     3901 tgctgttcac tccaatctgc ccacattccc tgtcgttcag accagttata cttccagatt
     3961 ctgcaaaact cgagttaaag attccagatg atgctcgaag caatgcatgg gtttcgtttg
     4021 atggaaagag aagacaacaa ctttcaaggg gagattcggt gagaatatac atgagccaac
     4081 atccactccc aactgtcaac aaatctgatc aaaccggtga ttggtttaga agcttaatcc
     4141 gttgcttaaa ctggaacgag cgtcttgatc aaaaggctct cggatatcta gacccagctt
     4201 tcttgtacaa agtggttgat aacagcatgg tgtctaaggg cgaagagctg attaaggaga
     4261 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     4321 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     4381 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
     4441 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     4501 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     4561 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     4621 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc gagatgctgt
     4681 accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc gtgggcgggg
     4741 gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct aagaacctca
     4801 agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag gccgacaaag
     4861 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     4921 tggggcacaa acttaattga gcttagagct cgaatttccc cgatcgttca aacatttggc
     4981 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     5041 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     5101 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     5161 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     5221 tggttccgga accaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     5281 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5341 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc
     5401 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     5461 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     5521 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     5581 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     5641 tgaacgatcg gggaaattcg ggggatcgat cccgcgaact gtggacgaga actgtgccac
     5701 caagcgtaag gccgttctct cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag
     5761 gcgttctcga aatcagctct tgttcggtcg gcatctactc tattcctttg ccctcggacg
     5821 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     5881 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     5941 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     6001 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     6061 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     6121 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     6181 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     6241 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     6301 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     6361 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     6421 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     6481 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     6541 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat gccccaatgt caagcacttc
     6601 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     6661 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     6721 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     6781 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctt attgcccccc
     6841 gggatcagat ctggattgag agtgaatatg agactctaat tggataccga ggggaattta
     6901 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     6961 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     7021 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     7081 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct
     7141 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     7201 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     7261 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     7321 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     7381 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     7441 cttgcggcag cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg
     7501 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     7561 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     7621 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     7681 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     7741 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     7801 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     7861 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     7921 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     7981 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     8041 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     8101 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     8161 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     8221 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     8281 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     8341 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     8401 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     8461 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     8521 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     8581 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     8641 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     8701 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     8761 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     8821 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     8881 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     8941 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     9001 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     9061 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9121 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9181 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9241 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     9301 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9361 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9421 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9481 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9541 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9601 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9661 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9721 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9781 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9841 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9901 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     9961 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    10021 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
    10081 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
    10141 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
    10201 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
    10261 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
    10321 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
    10381 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
    10441 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
    10501 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    10561 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
    10621 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    10681 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    10741 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    10801 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    10861 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    10921 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    10981 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    11041 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    11101 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    11161 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    11221 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    11281 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    11341 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    11401 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    11461 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    11521 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    11581 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    11641 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    11701 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    11761 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
    11821 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    11881 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    11941 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    12001 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    12061 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    12121 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    12181 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    12241 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    12301 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    12361 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    12421 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    12481 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    12541 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    12601 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    12661 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    12721 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    12781 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    12841 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    12901 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    12961 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    13021 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    13081 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    13141 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    13201 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    13261 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    13321 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    13381 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    13441 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    13501 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    13561 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    13621 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    13681 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    13741 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    13801 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    13861 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    13921 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    13981 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
