LOCUS       sequence-404963-       11553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium transformation. Rattus rattus biliverdin reductase
            A (BVRA) flanked with an N-terminal plastid transit peptide and a 
            C-terminal FLAG tag under the control of the 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
  TITLE     Bilirubin is produced nonenzymatically in plants to maintain 
            chloroplast redox status.
  JOURNAL   Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. 
            Epub 2023 Jun 7.
  PUBMED    37285441
REFERENCE   2  (bases 1 to 11553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-9"; volume: "9(23):eadh4787. doi"; pages: " 
            10.1126/sciadv.adh4787. Epub 2023 Jun 7"
COMMENT     Sequence Label: pGWB511-TP-BVRA
FEATURES             Location/Qualifiers
     source          1..11553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2358..2381
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2395..2419)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2430..2453
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2475..2727
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2760..2776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2760..2776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2773..2795)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2784..2800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2808..2814,2815..2832,2833..2838))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2853..2874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2910..3162)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3313..4338)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4390..4569)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5075..5099
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5620..6411
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6657..7245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7146..7165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7399..7416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7431..7571
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7497..7516)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7657..7679
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7915..8109
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8175..9248)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9677..10306)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgg cttcctctat gctctcttcc gctactatgg
     1261 ttgcctctcc ggctcaggcc actatggtcg ctcctttcaa cggacttaag tcctccgctg
     1321 ccttcccagc cacccgcaag gctaacaacg acattacttc catcacaagc aacggcggaa
     1381 gagttaactg catgcaggtg tggcctccga ttggaaagaa gaagtttgag actctctctt
     1441 accttcctga ccttaccggc ggtagcggcg gtatggatgc cgagccaaag aggaaatttg
     1501 gagtggtagt ggttggtgtt ggcagagctg gctcggtgag gctgagggac ttgaaggatc
     1561 cacgctctgc agcattcctg aacctgattg gatttgtgtc cagacgagag cttgggagcc
     1621 ttgatgaagt acggcagatt tctttggaag atgctctccg aagccaagag attgatgtcg
     1681 cctatatttg cagtgagagt tccagccatg aagactatat acggcagttt ctgcaggctg
     1741 gcaagcatgt cctcgtggaa taccccatga cactgtcatt tgcggcggcc caggagctgt
     1801 gggagctggc cgcacagaaa gggagagtcc tgcatgagga gcacgtggaa ctcttgatgg
     1861 aggaattcga attcctgaga agagaagtgt tggggaaaga gctactgaaa gggtctcttc
     1921 gcttcacagc tagcccactg gaagaagaga gatttggctt ccctgcgttc agcggcattt
     1981 ctcgcctgac ctggctggtc tccctcttcg gggagctttc tcttatttct gccaccttgg
     2041 aagagcgaaa agaggatcag tatatgaaaa tgaccgtgca gctggagacc cagaacaagg
     2101 gtctgctgtc atggattgaa gagaaagggc ctggcttaaa aagaaacaga tatgtaaact
     2161 tccagttcac ttctgggtcc ctggaggaag tgccaagtgt aggggtcaat aagaacattt
     2221 tcctgaaaga tcaggatata tttgttcaga agctcttagg ccaggtctct gcagaggacc
     2281 tggctgctga gaagaagcgc atcatgcatt gcctggggct ggccagcgac atccagaagc
     2341 tttgccacca gaagaaggac tacaaggatg acgatgacaa gtagggatat ctagacccag
     2401 ctttcttgta caaagtggtt gataacagcg actacaagga tgacgatgac aaggcttaga
     2461 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2521 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2581 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2641 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2701 gcgcggtgtc atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg
     2761 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2821 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga
     2881 attaggcgcg cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga
     2941 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     3001 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     3061 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     3121 attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgggggatc
     3181 gatcccgcga actgtggacg agaactgtgc caccaagcgt aaggccgttc tctcgcattt
     3241 gccttgctag gctcgcgcga gttgctggct gaggcgttct cgaaatcagc tcttgttcgg
     3301 tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     3361 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     3421 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     3481 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     3541 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     3601 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     3661 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     3721 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     3781 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     3841 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     3901 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     3961 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
     4021 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     4081 gctctcgctg aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     4141 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     4201 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     4261 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     4321 gagttcaggc tttttcatat cttattgccc cccgggatca gatctggatt gagagtgaat
     4381 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     4441 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     4501 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     4561 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     4621 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     4681 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     4741 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     4801 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     4861 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     4921 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     4981 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     5041 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     5101 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     5161 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     5221 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     5281 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     5341 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     5401 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     5461 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     5521 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     5581 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     5641 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     5701 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     5761 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     5821 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     5881 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     5941 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     6001 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     6061 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     6121 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     6181 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     6241 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     6301 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     6361 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     6421 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     6481 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     6541 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     6601 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6661 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6721 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6781 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6841 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6901 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6961 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7021 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7081 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7141 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7201 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7261 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7321 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7381 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     7441 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     7501 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     7561 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7621 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7681 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     7741 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     7801 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     7861 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     7921 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     7981 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     8041 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     8101 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     8161 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     8221 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     8281 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     8341 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     8401 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     8461 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     8521 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     8581 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     8641 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     8701 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     8761 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     8821 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     8881 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     8941 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     9001 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     9061 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     9121 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     9181 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     9241 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     9301 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     9361 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     9421 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     9481 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     9541 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     9601 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     9661 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     9721 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     9781 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     9841 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     9901 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     9961 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    10021 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    10081 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    10141 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    10201 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    10261 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    10321 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    10381 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    10441 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    10501 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    10561 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    10621 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    10681 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    10741 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    10801 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    10861 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    10921 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    10981 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    11041 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    11101 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    11161 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    11221 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    11281 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    11341 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    11401 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    11461 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    11521 catgcacata caaatggacg aacggataaa cct
//
