LOCUS       sequence-404965-       11091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium transformation. A plastid transit peptide fused 
            UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 
            35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ishikawa K, Xie X, Osaki Y, Miyawaki A, Numata K, Kodama Y
  TITLE     Bilirubin is produced nonenzymatically in plants to maintain 
            chloroplast redox status.
  JOURNAL   Sci Adv. 2023 Jun 9;9(23):eadh4787. doi: 10.1126/sciadv.adh4787. 
            Epub 2023 Jun 7.
  PUBMED    37285441
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-9"; volume: "9(23):eadh4787. doi"; pages: " 
            10.1126/sciadv.adh4787. Epub 2023 Jun 7"
COMMENT     Sequence Label: pGWB511-TP-UnaG-FLAG
FEATURES             Location/Qualifiers
     source          1..11091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1482..1898
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
                     KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
                     GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     CDS             1899..1922
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(1933..1957)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1968..1991
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2013..2265
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2298..2314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2298..2314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2311..2333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2322..2338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2346..2352,2353..2370,2371..2376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2391..2412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2448..2700)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2851..3876)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3928..4107)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4613..4637
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5158..5949
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6195..6783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6684..6703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6937..6954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6969..7109
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7035..7054)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7195..7217
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7453..7647
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7713..8786)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9215..9844)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gctttaaagg aaccaattca gtcgacatgg cttcctctat gctctcttcc gctactatgg
     1261 ttgcctctcc ggctcaggcc actatggtcg ctcctttcaa cggacttaag tcctccgctg
     1321 ccttcccagc cacccgcaag gctaacaacg acattacttc catcacaagc aacggcggaa
     1381 gagttaactg catgcaggtg tggcctccga ttggaaagaa gaagtttgag actctctctt
     1441 accttcctga ccttaccgat tccggaggct caggaggctc catggtcgag aaatttgttg
     1501 gcacctggaa gatcgcagac agccataatt ttggtgaata cctgaaagct atcggagccc
     1561 caaaggaatt aagcgatggt ggggatgcca cgacgccgac attgtacatc tcccagaagg
     1621 acggagacaa aatgacagtg aaaatagaga atggacctcc tacgttcctt gacactcaag
     1681 taaagttcaa attaggggag gagttcgacg aatttccttc tgatcgaaga aaaggcgtaa
     1741 aatctgtcgt gaacttggtg ggagagaagc tggtgtacgt acaaaagtgg gacggcaagg
     1801 agacgacgta tgtccgagag ataaaggacg gtaaactggt cgtgacactt acgatgggag
     1861 acgtcgtggc tgtgcgcagc taccggaggg cgacggaaga ctacaaggat gacgatgaca
     1921 agggatatct agacccagct ttcttgtaca aagtggttga taacagcgac tacaaggatg
     1981 acgatgacaa ggcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     2041 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     2101 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     2161 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     2221 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcrssaa ttggttccgg
     2281 aaccaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2341 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2401 taactcacat taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat
     2461 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     2521 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     2581 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     2641 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     2701 ggggaaattc gggggatcga tcccgcgaac tgtggacgag aactgtgcca ccaagcgtaa
     2761 ggccgttctc tcgcatttgc cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg
     2821 aaatcagctc ttgttcggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     2881 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     2941 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     3001 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     3061 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     3121 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     3181 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     3241 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     3301 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     3361 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     3421 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     3481 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     3541 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     3601 ggagatgcaa taggtcaggc tctcgctgaa tgccccaatg tcaagcactt ccggaatcgg
     3661 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     3721 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     3781 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     3841 ctcgacagac gtcgcggtga gttcaggctt tttcatatct tattgccccc cgggatcaga
     3901 tctggattga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
     3961 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
     4021 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
     4081 tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
     4141 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct
     4201 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     4261 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     4321 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     4381 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     4441 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     4501 gcgtgaagct gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc
     4561 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     4621 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     4681 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     4741 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     4801 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     4861 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     4921 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     4981 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     5041 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     5101 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     5161 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     5221 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     5281 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     5341 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     5401 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     5461 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     5521 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     5581 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     5641 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     5701 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     5761 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     5821 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     5881 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     5941 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     6001 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     6061 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     6121 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6301 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6901 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6961 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     7021 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     7081 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     7141 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     7201 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     7261 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     7321 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     7381 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     7441 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     7501 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     7561 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     7621 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     7681 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     7741 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     7801 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     7861 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     7921 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     7981 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     8041 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     8101 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
     8161 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
     8221 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
     8281 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
     8341 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
     8401 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
     8461 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
     8521 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
     8581 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
     8641 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
     8701 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
     8761 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
     8821 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     8881 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     8941 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     9001 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
     9061 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
     9121 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
     9181 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
     9241 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
     9301 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
     9361 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
     9421 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
     9481 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
     9541 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
     9601 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
     9661 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
     9721 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
     9781 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
     9841 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
     9901 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
     9961 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    10021 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    10081 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    10141 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    10201 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    10261 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    10321 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    10381 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    10441 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    10501 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    10561 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    10621 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    10681 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    10741 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    10801 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    10861 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    10921 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    10981 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
    11041 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc t
//
