LOCUS       sequence-404967-       16545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B WT fused with EmGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Wouters R, Beletchi I, Van den Haute C, Baekelandt V, Martin S, 
            Eggermont J, Vangheluwe P
  TITLE     The lipid flippase ATP10B enables cellular lipid uptake under stress
            conditions.
  JOURNAL   Biochim Biophys Acta Mol Cell Res. 2024 Feb;1871(2):119652. doi: 
            10.1016/j.bbamcr.2023.119652. Epub 2023 Dec 11.
  PUBMED    38086447
REFERENCE   2  (bases 1 to 16545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Cell Res."; date: "2024-02"; volume: 
            "1871(2):119652. doi"; pages: " 10.1016/j.bbamcr.2023.119652. Epub 
            2023 Dec 11"
COMMENT     Sequence Label: pLenti HsATP10B - EmGFP
FEATURES             Location/Qualifiers
     source          1..16545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1191..1210)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1424..1443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1543..1565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1603..1621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1794..1862,1863..2654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2012..2031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2825..3413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3314..3333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3567..3584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3654..3674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3659..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5122..5187
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5317..5337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5762..5896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5853..5872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6064..6697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6744..6869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7362..7595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7780..7824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8117..8234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8293..8596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8597..8800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8751..8771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8797..8821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8889..8898
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9930..9941)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(13297..13321)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(13335..13337,13338..13340,13341..14054)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(13380..13401)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(13641..13660)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13988..14009
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    14096..14648
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(14240..14257)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     14467..14486
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             14648..15250
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     15147..15167
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    15270..15858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(15323..15343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15741..15752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             16028..16208
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     4021 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     4081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     4141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     4201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     4261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     4321 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     4381 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     4441 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4501 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4561 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4621 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4681 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4741 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4801 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4861 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4921 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4981 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     5041 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5101 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5161 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5221 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5281 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5341 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5401 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5461 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5521 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5581 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5641 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5941 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     6001 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     6061 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     6121 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6181 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6241 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6301 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6361 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6421 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6481 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6661 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6721 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6781 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6841 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7201 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7261 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7321 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7381 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7441 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7501 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7561 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7621 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7681 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7741 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7801 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7861 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7921 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7981 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8041 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     8101 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     8161 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     8221 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ggcgataagc
     8281 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8401 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8581 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     8641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     8701 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     8761 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     8821 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagaggat
     8881 ctgctagcgc caccatggcc ctctcagtgg actcatcgtg gcatcggtgg cagtggagag
     8941 tcagagatgg cttcccccat tgtccatcgg aaaccacacc gctgctctct ccagagaaag
     9001 ggagacagag ctacaacttg acacagcagc gggtcgtgtt ccccaacaac agcatattcc
     9061 atcaagattg ggaagaggtc tccaggagat accctggcaa cagaacctgc acaaccaaat
     9121 acaccctctt caccttcctg ccccggaatc tctttgagca atttcataga tgggctaacc
     9181 tctatttcct gttcctggtg attttgaact ggatgccctc catggaagtc ttccacagag
     9241 aaatcaccat gttaccattg gccattgtcc tgttcgtcat catgatcaag gatggcatgg
     9301 aggacttcaa gagacaccgc tttgataaag caataaactg ctccaacatt cgaatttatg
     9361 aaagaaaaga gcagacctat gtgcagaagt gctggaagga tgtgcgcgtg ggagacttca
     9421 tccaaatgaa atgcaatgag attgtcccag cagacatact cctccttttt tcctctgacc
     9481 ccaatgggat atgccatctg gaaactgcca gcttggatgg agagacaaac ctcaagcaaa
     9541 gatgtgtcgt gaagggcttc tcacagcagg aggtacagtt cgaaccagag cttttccaca
     9601 ataccatcgt gtgtgagaaa cccaacaacc acctcaacaa atttaagggt tatatggagc
     9661 atcctgacca gaccaggact ggctttggct gtgagagtct tctgcttcga ggctgcacca
     9721 tcagaaacac cgagatggct gttggcattg tcatctatgc aggccatgag acgaaagcca
     9781 tgctgaacaa cagtggcccc cggtacaaac gcagcaagat tgagcggcgc atgaatatag
     9841 acatcttctt ctgcattggg atcctcatcc tcatgtgcct tattggagct gtaggtcaca
     9901 gcatctggaa tgggaccttt gaagaacacc ctcccttcga tgtgccagat gccaatggca
     9961 gcttccttcc cagtgccctt gggggcttct acatgttcct cacaatgatc atcctgctcc
    10021 aggtgctgat ccccatctct ttgtatgtct ccattgagct ggtgaagctc gggcaagtgt
    10081 tcttcttgag caatgacctt gacctgtatg atgaagagac cgatttatcc attcaatgtc
    10141 gagccctcaa catcgcagag gacttgggcc agatccagta catcttctcc gataagacgg
    10201 ggaccctgac agagaacaag atggtgttcc gacgttgcac catcatgggc agcgagtatt
    10261 ctcaccaaga aaatgctaag cgactggaga ccccaaagga gctggactca gatggtgaag
    10321 agtggaccca ataccaatgc ctgtccttct cggctagatg ggcccaggat ccagcaacta
    10381 tgagaagcca aaaaggtgct cagcctctga ggaggagcca gagtgcccgg gtgcccatcc
    10441 agggccacta ccggcaaagg tctatggggc accgtgaaag ctcacagcct cctgtggcct
    10501 tcagcagctc catagaaaaa gatgtaactc cagataaaaa cctactgacc aaggttcgag
    10561 atgctgccct gtggttggag accttgtcag acagcagacc tgccaaggct tccctctcca
    10621 ccacctcctc cattgctgat ttcttccttg ccttaaccat ctgcaactct gtcatggtgt
    10681 ccacaaccac cgagcccagg cagagggtca ccatcaaacc ctcaagcaag gctctgggga
    10741 cgtccctgga gaagattcag cagctcttcc agaagttgaa gctattgagc ctcagccagt
    10801 cattctcatc cactgcaccc tctgacacag acctcgggga gagcttaggg gccaacgtgg
    10861 ccaccacaga ctcggatgag agagatgatg catctgtgtg cagtggaggt gactccactg
    10921 atgacggtgg ctacaggagc agcatgtggg accagggcga catcctggag tctgggtcag
    10981 gcacttcctt ggaggaggca ttggaggccc cagccacaga cctggccagg cctgagttct
    11041 gttacgaggc tgagagccct gatgaggccg ccctggtgca cgctgcccat gcctacagct
    11101 tcacactagt gtcccggaca cctgagcagg tgactgtgcg cctgccccag ggcacctgcc
    11161 tcaccttcag cctcctctgc accctgggct ttgactctgt caggaagaga atgtctgtgg
    11221 ttgtgaggca cccactgact ggcgagattg ttgtctacac caagggtgct gactcggtca
    11281 tcatggacct gctggaagac ccagcctgcg tacctgacat taatatggaa aagaagctga
    11341 gaaaaatccg agcccggacc caaaagcatc tagacttgta tgcaagagat ggcctgcgca
    11401 cactatgcat tgccaagaag gttgtaagcg aagaggactt ccggagatgg gccagtttcc
    11461 ggcgtgaggc tgaggcatcc ctcgacaacc gagatgagct tctcatggaa actgcacagc
    11521 atctggagaa tcaactcacc ttacttggag ccactgggat cgaagaccgg ctgcaggaag
    11581 gagttccaga tacgattgcc actctgcggg aggctgggat ccagctctgg gtcctgactg
    11641 gagataagca ggagacagcg gtcaacattg cccattcctg cagactgtta aatcagaccg
    11701 acactgttta taccatcaat acagagaatc aggagacctg tgaatccatc ctcaattgtg
    11761 cattggaaga gctaaagcaa tttcgtgaac tacagaagcc agaccgcaag ctctttggat
    11821 tccgcttacc ttccaagaca ccatccatca cctcagaagc tgtggttcca gaagctggat
    11881 tggtcatcga tgggaagaca ttgaatgcca tcttccaggg aaagctagag aagaagtttc
    11941 tggaattgac ccagtattgt cggtccgtcc tgtgctgccg ctccacgcca ctccagaaga
    12001 gtatgatagt caagctggtg cgagacaagt tgcgcgtcat gaccctttcc ataggtgatg
    12061 gagcaaatga tgtaagcatg attcaagctg ctgatattgg aattggaata tctggacagg
    12121 aaggcatgca ggctgtcatg tccagcgact ttgccatcac ccgctttaag catctcaaga
    12181 agttgctgct cgtgcatggc cactggtgtt actcgcgcct ggccaggatg gtggtgtact
    12241 acctctacaa gaacgtgtgc tacgtcaacc tgctcttctg gtatcagttc ttctgtggtt
    12301 tctccagctc caccatgatt gattactggc agatgatatt cttcaatctc ttctttacct
    12361 ccttgcctcc tcttgtcttt ggagtccttg acaaagacat ctctgcagaa acactcctgg
    12421 cattgcctga gctatacaag agtggccaga actctgagtg ctataacctg tcgactttct
    12481 ggatttctat ggtggatgca ttctaccaga gcctcatctg tttctttatc ccttacctgg
    12541 cctataaggg ctctgatata gatgtcttta cctttgggac accaatcaac accatctccc
    12601 tcaccacaat ccttttgcac caggcaatgg aaatgaagac atggaccatt ttccacggag
    12661 tcgtgctcct cggcagcttc ctgatgtact ttctggtatc cctcctgtac aatgccacct
    12721 gcgtcatctg caacagcccc accaatccct attgggtgat ggaaggccag ctctcaaacc
    12781 ccactttcta cctcgtctgc tttctcacac cagttgttgc tcttctccca agatactttt
    12841 tcctgtctct gcaaggaact tgtgggaagt ctctaatctc aaaagctcag aaaattgaca
    12901 aactcccccc agacaaaaga aacctggaaa tccagagttg gagaagcaga cagaggcctg
    12961 cccctgtccc cgaagtggct cgaccaactc accacccagt gtcatctatc acaggacagg
    13021 acttcagtgc cagcacccca aagagctcta accctcccaa gaggaagcat gtggaagagt
    13081 cagtactcca cgaacagaga tgtggcacgg agtgcatgag ggatgactca tgctcagggg
    13141 actcctcagc tcaactctca tccggggagc acctgctggg acctaacagg ataatggcct
    13201 actcaagagg acagactgat atgtgccggt gctcaaagag gagcagccat cgccgatccc
    13261 agagttcact gaccatatac ctcgagtgcg gccgcaaccc agctttcttg tacaaagtgg
    13321 ttgatgctgt taacatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
    13381 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
    13441 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
    13501 tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc gcccgctacc
    13561 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
    13621 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
    13681 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
    13741 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
    13801 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
    13861 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
    13921 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
    13981 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
    14041 agctgtacaa gtaaacgcgt gctctagccc aattccgccc cctccccccc ccctaacgtt
    14101 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
    14161 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
    14221 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
    14281 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
    14341 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
    14401 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
    14461 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
    14521 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
    14581 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
    14641 tgataatatg ggcaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    14701 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    14761 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    14821 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
    14881 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    14941 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    15001 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    15061 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    15121 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    15181 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    15241 cggtgcctga ggcgcgttga attcccgata atcaacctct ggattacaaa atttgtgaaa
    15301 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
    15361 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
    15421 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
    15481 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
    15541 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
    15601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    15661 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    15721 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    15781 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    15841 tttgggccgc ctccccgcat cgggaattct gtcgactggt acctttaaga ccaatgactt
    15901 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    15961 ttcactccca acgaagacaa aatcgtcgag agatgctgca tataagcagc tgctttttgc
    16021 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    16081 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    16141 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    16201 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
    16261 aatatcagag agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag
    16321 tggagcacac aggcaaagct gcagaagtac ttggaagaag ccaccagaga tactcacgat
    16381 tctgcacata cctggctaat cccagatcct aaggattaca ttaagtttac taacatttat
    16441 ataatgattt atagtttaaa gtataaactt atctaattta ctattctgac agatattaat
    16501 taatcctcaa atatcataag agatgattac tattatcccc attta
//
