LOCUS       sequence-404974-        9284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human long non-coding RNA H19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Hofmann P, Sommer J, Theodorou K, Kirchhof L, Fischer A, Li Y, 
            Perisic L, Hedin U, Maegdefessel L, Dimmeler S, Boon RA
  TITLE     Long non-coding RNA H19 regulates endothelial cell aging via 
            inhibition of STAT3 signalling.
  JOURNAL   Cardiovasc Res. 2019 Jan 1;115(1):230-242. doi: 10.1093/cvr/cvy206.
  PUBMED    30107531
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Cardiovasc Res"; date: "2019-01-1- 1"; volume: "115"; issue: "1"; 
            pages: "230-242"
COMMENT     Sequence Label: pLenti4-Puro-H19
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      67..202
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             226..825
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(226..245)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     722..742
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             897..1130
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1202..1323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1239..1258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1293..1312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1363..1498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1518..1537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1519..1537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1547..1564)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1547..1563)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1556..1578)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1705..2160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1792..1811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2002..2023
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2186..2290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2291..2359,2360..3151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2509..2528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3322..3910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3811..3830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4064..4081
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4198..4219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4234..4239,4240..4257,4258..4264)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4272..4288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4277..4299
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4296..4312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4296..4312
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4331..4351
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4333..4351
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4379..4605
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4606..4786
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4833..4958
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5451..5684
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5869..5913
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6495..6698
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6649..6669
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6700..6718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6721..6739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             9046..9087
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9097..9114
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9137..9154)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(9166..9186)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9171..9284,1..216)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
       61 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
      121 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
      181 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag
      241 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
      301 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
      361 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
      421 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
      481 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
      541 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
      601 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
      661 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
      721 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
      781 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaggtac ctttaagacc
      841 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
      901 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
      961 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1021 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1081 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     1141 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     1201 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1261 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1321 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     1381 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1441 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1501 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     1561 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1621 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1681 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1741 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1801 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1861 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1921 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     1981 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2041 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2101 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     2161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2281 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2341 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2401 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2461 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2521 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2581 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2641 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2701 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2761 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2821 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2881 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2941 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3001 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3061 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3121 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3181 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3241 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3721 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3781 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3841 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3901 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3961 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4021 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4081 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4141 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4201 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4261 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     4321 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     4381 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     4441 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     4501 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     4561 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     4621 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4681 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4741 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4801 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4861 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4921 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4981 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     5041 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     5101 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     5161 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     5221 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     5281 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5341 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5401 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5461 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5521 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5581 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5641 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5701 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5761 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5821 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5881 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5941 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     6001 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     6061 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     6121 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgataagct
     6181 tgggagttcc gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     6241 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     6301 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     6361 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     6421 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     6481 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     6541 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     6601 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     6661 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
     6721 tccctatcag tgatagagat cgtcgactag tggggtaacg ggggaaactg gggaagtggg
     6781 gaaccgaggg gcaaccaggg gaagatgggg tgctggagga gagcttgtgg gagccaagga
     6841 gcaccttgga catctggagt ctggcaggag tgatgacggg tggaggggct agctcgaggc
     6901 agggctggtg gggcctgagg ccagtgagga gtgtggagta ggtgcccagg catcgtgcag
     6961 acagggcgac atcagctggg gacgatgggc ctgagctagg gctggaaaga agggggagcc
     7021 aggcattcat cccggtcact tttggttaca ggacgtggca gctggttgga cgaggggagc
     7081 tggtgggcag ggtttgatcc cagggcctgg gcaacggagg tgtagctggc agcagcgggc
     7141 aggtgaggac cccatctgcc gggcaggtga gtcccttccc tccccaggcc tcgcttcccc
     7201 agccttctga aagaaggagg tttaggggat cgagggctgg cggggagaag cagacaccct
     7261 cccagcagag gggcaggatg ggggcaggag agttagcaaa ggtgacatct tctcgggggg
     7321 agccgagact gcgcaaggct ggggggctat gggcccgttc caggcagaaa gagcaagagg
     7381 gcagggaggg agcacagggg tggccagcgt agggtccagc acgtggggtg gtaccccagg
     7441 cctgggtcag acagggacaa ggcaggggac acaggacaga ggggtcccca gctgccacct
     7501 cacccaccgc aattcattta gtagcaggca caggggcagc tccggcacgg ctttctcagg
     7561 cctatgccgg agcctcgagg gctggagagc gggaagacag gcagtgctcg gggagttgca
     7621 gcaggacatc accaggaggg cgaagcggcc acgggagggg ggccccggga cattgcgcag
     7681 caaggaggct gcaggggctc ggcctgcggg cgccggtccc acgaggcact gcggcccagg
     7741 gtctggtgcg gagagggccc acagtggact tggtgacgct gtatgccctc accgctcagc
     7801 ccctggggct ggcttggcag acagtacagc atccagggga gtcaagggca tggggcgaga
     7861 ccagactagg cgaggcgggc ggggcggagt gaatgagctc tcaggaggga ggatggtgca
     7921 ggcaggggtg aggagcgcag cgggcggcga gcgggaggca ctggcctcca gagcccgtgg
     7981 ccaaggcggg cctcgcgggc ggcgacggag ccgggatcgg tgcctcagcg ttcgggctgg
     8041 agacgaggcc aggtctccag ctggggtgga cgtgcccacc agctgccgaa ggccaagacg
     8101 ccaggtccgg tggacgtgac aagcaggaca tgacatggtc cggtgtgacg gcgaggacag
     8161 aggaggcgcg tccggccttc ctgaacacct taggctggtg gggctgcggc aagaagcggg
     8221 tctgtttctt tacttcctcc acggagtcgg cacactatgg ctgccctctg ggctcccaga
     8281 acccacaaca tgaaagaaat ggtgctaccc agctcaagcc tgggcctttg aatccggaca
     8341 caaaaccctc tagcttggaa atgaatatgc tgcactttac aaccactgca ctacctgact
     8401 caggaatcgg ctctcgaagg tgaagcgaga ggaaccagac ctcatcagcc caacatcaaa
     8461 gacaccatcg gaacagcagc gcccgcagca cccaccccgc accggcgact ccatcttcac
     8521 ggccaccccc tgcggcggac ggttgaccac cagccaccac atcatcccag agctgagctc
     8581 ctccagcggg atgacgccgt ccccaccacc tccctcttct tctttttcat ccttctgtct
     8641 ctttgtttct gagctttcct gtctttcctt tttttctgag agattcaaag cctccacgac
     8701 tctgtttccc ccgtcccttc tgaatttaat ttgcactaag tcatttgcac tggttggagt
     8761 tgtggagacg gccttgagtc tcggtgcgag tgtgcgtgag tgtgagccac cttggcaagt
     8821 gcctgcgcag ggcccggccg ccctccatct gggccgggtg actgggcgcc ggctgtgtgc
     8881 ccgaggcctc accctgccct cgcctagtct ggaagctccg accgacatca cggagcagcc
     8941 ttcaagcatt ccattacgcc ccatctcgct ctgtgcccct ccccaccagg gcttcagcag
     9001 gagccctgga ctcatcatca ataaacactg ttacagcaat tcgaaggtaa gcctatccct
     9061 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     9121 taaacctgct gatcagcctc gactgtgcct tctagtcgac cctgtggaat gtgtgtcagt
     9181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agca
//
