LOCUS       sequence-404993-        9306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of full-length NUT 
            tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-NUT
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1058..1084
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1088..1792
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     primer_bind     complement(5258..5275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5264..5488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5621..5640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5772..5819
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5827..6849
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5827..6849
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6979..7100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7016..7035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7070..7089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7149..7165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7149..7165)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7162..7184)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7173..7189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7197..7203,7204..7221,7222..7227))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7242..7263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7380..7397)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7551..8139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7631..7650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8310..9101,9102..9170))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8933..8952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9171..9275)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatccagcac agtggcggcc gatgggaccg gctgccaagc gcgtgaaact
     1081 cgatggcgtg agcaagggcg aggaggataa catggcctct ctcccagcga cacatgagtt
     1141 acacatcttt ggctccatca acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa
     1201 tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc tccagttctc
     1261 cccctggatt ctggtccctc atatcgggta tggcttccat cagtacctgc cctaccctga
     1321 cgggatgtcg cctttccagg ccgcaatggt agatggctcc ggataccaag tccatcgcac
     1381 aatgcagttt gaagatggtg cctcccttac tgttaactac cgctacacct acgagggaag
     1441 ccacatcaaa ggagaggccc aggtgaaggg gactggtttc cctgctgacg gtcctgtgat
     1501 gaccaactcg ctgaccgctg cggactggtg caggtcgaag aagacttacc ccaacgacaa
     1561 aaccatcatc agtaccttta agtggagtta caccactgga aatggcaagc gctaccggag
     1621 cactgcgcgg accacctaca cctttgccaa gccaatggcg gctaactatc tgaagaacca
     1681 gccgatgtac gtgttccgta agacggaact caagcactcc aagaccgaac tcaacttcaa
     1741 ggagtggcaa aaggccttta ccgatgtgat gggcatggac gagctgtaca agggcggctc
     1801 gtacgagggg tcgagcgcat ctgcattgcc gggaccggat atgagcatga aacctagtgc
     1861 cgccctgtct ccatcccctg cacttccctt tctcccacca acttctgacc caccagacca
     1921 cccacccagg gagccacctc cacagcccat catgccttca gtattctctc cagacaaccc
     1981 tctgatgctc tctgctttcc ccagctcact gttggtgaca ggggacgggg gcccttgcct
     2041 cagtggggct ggggctggca aggtcattgt caaagtcaag acagaagggg ggtcagctga
     2101 gccctctcaa actcagaact ttatccttac tcagactgcc ctcaattcga ctgccccggg
     2161 cactccctgt ggaggccttg agggtcctgc acctccattt gtgacagcat ctaatgtgaa
     2221 gaccattctg ccctctaagg ctgttggtgt cagccaggag ggtcctccag gccttccgcc
     2281 tcagcctcca ccaccagttg ctcaactggt ccccattgtg cccctggaaa aagcttggcc
     2341 agggccacat gggacaaccg gggaaggagg tcctgtggcc actctatcca agccttccct
     2401 aggtgaccgc tccaaaattt ccaaggacgt ttatgagaac ttccgtcagt ggcagcgtta
     2461 caaagccttg gcccggaggc acctatccca gagtcctgac acagaagctc tttcctgttt
     2521 tcttatccca gtgcttcgtt ccctggcccg gctgaagccc actatgaccc tggaggaggg
     2581 actgccattg gctgtgcagg agtgggagca caccagcaac tttgaccgga tgatctttta
     2641 tgagatggca gaaaggttca tggagtttga ggctgaggag atgcagattc agaacacaca
     2701 gctgatgaat gggtctcagg gcctgtctcc tgcaacccct ttgaaacttg atcctctagg
     2761 gcccctggcc tctgaggttt gccagcagcc agtgtacatt ccgaagaagg cagcctccaa
     2821 gacacgggcc ccccgccggc gtcagcgtaa agcccagaga cctcctgctc ctgaggcacc
     2881 caaggagatc ccaccagaag ctgtgaagga gtatgttgac atcatggaat ggctggtggg
     2941 gactcacttg gccactgggg agtcagatgg aaaacaagag gaagaagggc agcagcagga
     3001 ggaggaaggg atgtatccag atccaggtct cctgagctac atcaatgagc tgtgttctca
     3061 gaaggtcttt gtctccaagg tggaggctgt cattcaccct caatttctgg cagatctgct
     3121 gtccccagaa aaacagagag atcccttggc cttaattgag gagctagagc aagaagaagg
     3181 actcactctt gcccagctgg tccagaagcg actcatggcc ttggaagagg aggaagatgc
     3241 agaggcgcct ccaagtttca gtggcgctca gttggactca agtccttctg gttctgttga
     3301 ggatgaagat ggggatgggc ggcttcggcc ctcacctggg cttcaggggg ctgggggcgc
     3361 cgcttgcctt ggaaaggttt cttcttcagg aaaacgggca agagaagtgc atggtgggca
     3421 ggagcaagcc ctagatagcc ccagagggat gcacagggat gggaacactc tgccatcccc
     3481 cagcagctgg gacctgcagc cagaacttgc agctccacag ggaactccgg gacccttggg
     3541 tgtggagagg agagggtctg ggaaggttat aaaccaggta tctctacatc aggatggcca
     3601 tctaggaggc gctgggcctc ctgggcactg cctggtggct gataggactt cagaggctct
     3661 gcccctttgt tggcagggag gcttccagcc tgagagcact cccagtttgg atgctggact
     3721 tgcagagctg gctcctctgc aaggacaagg gttagaaaag caagtcctgg gattgcagaa
     3781 aggacaacaa acagggggtc gtggagtgct tcctcaaggg aaggagcctt tagcagtgcc
     3841 ctgggaaggc tcttcaggag ccatgtgggg agatgacaga ggtaccccca tggctcagag
     3901 ttatgatcag aatccttccc ctagagcagc tggggagagg gacgatgtct gtctcagccc
     3961 aggagtttgg ctgagcagtg agatggatgc tgtaggcttg gagctgcctg tacaaataga
     4021 ggaggtcata gagagcttcc aagttgagaa gtgtgtaact gagtatcagg aaggctgcca
     4081 gggactgggc tccaggggca acatttccct gggtcctgga gaaaccctag tacctgggga
     4141 tacggagagc agtgtgattc cctgtggagg cacagttgcg gcagctgccc tagaaaagag
     4201 aaactattgc agcttgccag gacctttgag ggccaacagc ccacccttga ggtccaaaga
     4261 aaatcaagaa cagagctgtg aaaccgtagg gcatcccagt gatctgtggg cagaaggttg
     4321 cttcccattg ctagaaagtg gtgattccac actggggtct tccaaagaaa cccttccacc
     4381 cacatgccaa ggcaatctcc ttatcatggg gactgaggat gcctcctcct tgcctgaagc
     4441 cagccaagag gcagggagca gaggcaattc cttttctcct ctgttggaaa ccatagaacc
     4501 tgtcaacata ctagatgtta aagatgactg tggcctccaa ctaagggtca gcgaggacac
     4561 ctgcccactg aatgttcatt cttatgaccc ccaaggagaa ggcagggtgg atcctgatct
     4621 gtccaagcct aaaaaccttg ctcctttaca agagagtcag gagtcttaca caactgggac
     4681 tcccaaagca acatcttctc accagggcct tggaagcact ttgcctagaa ggggaaccag
     4741 gaatgccata gttccgagag aaacttctgt tagtaaaaca cacaggtcag cagacagggc
     4801 caaaggaaag gagaaaaaga aaaaggaagc agaggaagag gatgaggaac tctccaactt
     4861 tgcttacctc ttggcctcta aacttagcct ctcaccaagg gagcatcccc tcagtcctca
     4921 ccatgcctca ggaggtcagg gcagccagag agcatcccac ctgctccctg ctggagcaaa
     4981 aggccccagc aaacttccat atcctgttgc caagtctggg aagcgagctc tagctggagg
     5041 tccagcccct actgaaaaga caccccactc aggagctcaa cttggggtcc ccagggagaa
     5101 acccctagct ctgggagtag ttcgaccctc acagcctcgt aaaaggcggt gtgacagttt
     5161 tgtcacgggc agaaggaaga aacgacgtcg tagccagggc tcgacctgat catagtgagg
     5221 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5281 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5341 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5401 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5461 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5521 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5641 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5701 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5761 tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
     5821 ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     5881 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     5941 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     6001 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     6061 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     6121 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     6181 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     6241 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     6301 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     6361 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     6421 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     6481 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     6541 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     6601 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     6661 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     6721 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     6781 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     6841 aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6901 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6961 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7081 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7141 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7201 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7261 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7321 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7981 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8341 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8401 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8461 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8521 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8581 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8641 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8701 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8761 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8821 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8881 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8941 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9001 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9061 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9301 gacgtc
//
