LOCUS       sequence-405028-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prime editor (PE2) with codon-optimized Reverse transcriptase for 
            prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Gao Z, Ravendran S, Mikkelsen NS, Haldrup J, Cai H, Ding X, Paludan 
            SR, Thomsen MK, Mikkelsen JG, Bak RO
  TITLE     A truncated reverse transcriptase enhances prime editing by split 
            AAV vectors.
  JOURNAL   Mol Ther. 2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 
            10.1016/j.ymthe.2022.07.001.
  PUBMED    35808824
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2022 Jul 8. pii: S1525-0016(22)00422-1. doi: 
            10.1016/j.ymthe.2022.07.001."
COMMENT     Sequence Label: PE_CO
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        402..781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        782..985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     936..956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1107..1127
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1128..3641,3642..3644,3645..5228)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1128..5228
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7401..7421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7430..7447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7460..7515
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7725..7741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7725..7741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7749..7765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7773..7779,7780..7797,7798..7803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7818..7839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7956..7973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8127..8715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8207..8226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8886..9677,9678..9746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9509..9528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9747..9851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9919..9937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgacattgat tattgactag
      421 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      481 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      541 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      601 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      661 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      721 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      781 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      841 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      901 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      961 gtgggaggtc tatataagca gagctgagct cggtacctaa tacgactcac tataaggaaa
     1021 taagagagaa aagaagagta agaagaaata tagctagcag agccgccacc atgaaacgga
     1081 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
     1141 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     1201 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     1261 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     1321 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1381 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1441 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1501 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1561 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1621 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1681 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1741 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     1801 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1861 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1921 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1981 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     2041 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     2101 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     2161 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     2221 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     2281 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     2341 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2401 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2461 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2521 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2581 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2641 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2701 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2761 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2821 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2881 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2941 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     3001 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     3061 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     3121 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     3181 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     3241 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     3301 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3361 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3421 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3481 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3541 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3601 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3661 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3721 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3781 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3841 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3901 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3961 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     4021 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     4081 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     4141 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     4201 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     4261 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     4321 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4381 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4441 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4501 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4561 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4621 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4681 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4741 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4801 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4861 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4921 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4981 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     5041 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     5101 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     5161 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     5221 gaggtgactc tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg
     5281 agtcagcaac accagagagc agtggcggca gcagcggcgg cagcagcacc ctgaacatcg
     5341 aagatgagta cagactgcac gagacatcta aggaacctga tgtgtccctg ggttctacat
     5401 ggctgagcga cttccctcag gcctgggctg aaaccggcgg catgggcctg gctgtgcggc
     5461 aggctcctct gatcatccct ctgaaggcca cctccacccc tgtgtccatc aagcagtacc
     5521 ccatgagcca ggaggccaga ctgggcatca agccccacat ccagcggctg ctcgaccagg
     5581 gaatcctggt cccctgccag agcccctgga acaccccact tttacctgtg aaaaagcccg
     5641 gcactaacga ctaccggcct gtgcaggacc tgagagaagt gaacaagcgg gttgaggaca
     5701 tccaccctac agtgcctaac ccctacaacc tgctgtctgg cctgccaccg agccaccagt
     5761 ggtacaccgt gctggacctg aaagacgcct tcttctgtct gagactccat cctaccagcc
     5821 agccactgtt cgccttcgag tggcgggacc ctgagatggg catcagcggc cagctgacat
     5881 ggacccggct gccacagggc ttcaagaaca gccctacact gttcaacgag gccctgcaca
     5941 gagatctggc tgacttcaga atccagcatc ctgacctgat cctgctgcag tacgtggacg
     6001 acttgctgct tgccgctaca tctgaactgg attgccagca aggcaccaga gctctgctcc
     6061 agaccctggg caacctgggg tatagggcca gcgccaagaa ggcccagatc tgccagaagc
     6121 aagtgaagta cctgggatac ctgctgaagg aaggccagag atggctgacc gaggcgcgga
     6181 aggaaacagt gatgggccag cctaccccta agacccccag acagctgcga gaattcctgg
     6241 gcaaggctgg cttttgcaga ctgttcatcc ccggcttcgc tgagatggcc gctcctctgt
     6301 acccactgac caagcccggt accctgttca attggggccc cgatcagcag aaagcctacc
     6361 aggagattaa gcaggccctg ctgaccgccc ctgccctcgg gctgcctgat ctgacaaaac
     6421 ctttcgagct gtttgtcgac gagaagcagg gctacgccaa gggcgtgctg acacagaaac
     6481 tgggaccttg gagaagacct gtggcttatc tcagcaagaa gctggacccc gtggccgccg
     6541 gatggcctcc atgtctgagg atggtcgccg ctatcgccgt gctgaccaag gacgccggca
     6601 aactgaccat gggacaacct ctggtgatcc tggcccctca cgccgtcgag gccctggtga
     6661 agcagcctcc tgacagatgg ctgagcaacg ccagaatgac ccactaccag gccctgctgc
     6721 tggataccga tagagtgcag ttcggccctg tggtggctct gaatcctgcc accctgctcc
     6781 cacttcccga ggaaggcctg cagcacaact gcctggacat cctggcagaa gcccacggca
     6841 caagacctga cttaacagac cagcctctgc ctgatgccga ccacacctgg tataccgacg
     6901 gcagcagcct gctgcaggag ggccaaagaa aggccggagc cgccgtgaca acagaaaccg
     6961 aggtgatctg ggccaaggcc ctccctgctg gcaccagcgc ccaaagagcc gagctgattg
     7021 ccctcaccca ggccctgaag atggccgaag gcaagaaact gaatgtgtac actgactcta
     7081 gatacgcctt tgccacagcc cacatccacg gcgagatcta cagacggaga ggatggctaa
     7141 cctctgaggg caaggaaatc aaaaacaagg atgagattct ggccctgctg aaggcactgt
     7201 ttctgcccaa gagactgagc atcatccact gccccggcca ccagaaggga cacagcgccg
     7261 aggcccgggg caatagaatg gccgatcagg ccgctcggaa ggccgccatc accgaaacac
     7321 ccgacaccag cacacttctg atcgagaact ccagcccctc tggcggctca aaaagaaccg
     7381 ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctaaccggtc atcatcacca
     7441 tcaccattga ggcggccgca ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     7501 tttttgtgtc tctcattaat taagctgcct tctgcggggc ttgccttctg gccatgccct
     7561 tcttctctcc cttgcacctg tacctcttgg tctttgaata aagcctgagt aggaagtcta
     7621 gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagggtcttc
     7681 atcatcgcct gcagaggcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     7741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9901 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
