LOCUS       sequence-405029-       14072 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter 
            (pan-neuronal expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Farrants H, Shuai Y, Lemon WC, Hernandez CM, Yang S, Patel R, Qiao 
            G, Frei MS, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, 
            Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER
  TITLE     A modular chemigenetic calcium indicator enables in vivo functional 
            imaging with near-infrared light.
  JOURNAL   bioRxiv. 2023 Jul 19:2023.07.18.549527. doi: 
            10.1101/2023.07.18.549527. Preprint.
  PUBMED    37503182
REFERENCE   2  (bases 1 to 14072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Jul 19:2023.07.18.549527. doi: 10.1101/2023.07.18.549527. 
            Preprint."
COMMENT     Sequence Label: pTol2-elavl3-WHaloCaMP1a
FEATURES             Location/Qualifiers
     source          1..14072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             9533..9550
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             10190..10630
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     polyA_signal    11029..11163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11053..11072)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11107..11126
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(11602..11789,11790..11801)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11835..11851)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11835..11851)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11888..11908)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11888..11906)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11927..11943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11927..11943)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11940..11962)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11951..11967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11975..11981,11982..11999,12000..12005))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12020..12041
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12158..12175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12329..12917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12409..12428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13088..13879,13880..13948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13711..13730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13949..14053)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttcccg
      721 ggtgtcgacg aattctcgag ctctattcct aaagaccttg ggtgaccaaa atcttatttt
      781 aataaataaa actgtttatt aaaacttttt tgtttcaaag aaccatatgt atagtgaaat
      841 ttataaaaat atcaattttt aaaaagctgg tgtactcatt tatgttatga actctaaaac
      901 catatactga ctgcaagtga tgatgtatag agtgatgttt acgagtaaac atatttagtt
      961 gtatacatcc tactgagcac attttgatgt atgaaataac attacaagct ttatccaaat
     1021 taagccattt taaaacactg ccaattgaaa atacaaatcc tggaaaaaat cgtctttagc
     1081 gcagtcattt gagccatcct aatccgttac ctcagaccat aataagaagg gataacacta
     1141 gctgtagcaa tggaacacat ctgtttcaca caatcatatc tcctgcgccg gtgctaagca
     1201 gattcagcgt gatcataaca tgctttccac tcataaatgt aaatttacaa tttgcacatg
     1261 taaaacagac acttttgaga tattggataa aaaaacaaga gtatattgct tagtttcatc
     1321 caccagtcat ccccacagcg tttggaaggc cataaaaagt gtctaaaatc aatgatcatt
     1381 gaaagagcac aagagagact cttacgctgt aatgccactg gggacaaaag tgacagtctc
     1441 ttaatgggct cttctggagg ggctcctgaa cattaaaaat tatcagcgaa attaccgaaa
     1501 gagcttcaag caactggcat gcttgatcct ctgcgtcggg gcggtgaata ggtgcttcag
     1561 atgccctctt acccacgggc tggattcagc tgccccgcta ccagcggaga ccccctaatg
     1621 agcctctgca attaagttta ttcatgttaa gtgtgaacgg ggtgcgtgcg gaactgtggg
     1681 cagctaacag acctgggttc tttgtgccac aagtgctgcc tttattcggc tcacaaagca
     1741 gaaaacaaca cccgcaccta taatggcgcc ctcggctggg tctaagaaac gtggcgagtt
     1801 gacagagcag agtgggcggg gttaagacag actgacagcg ggacccatct ccatcctctt
     1861 attaacgctt aacgagtgcc ttcctcatgc aatattcatc gccactaata tcatccaagc
     1921 tctgagctga gctggccact tatgtaaggc aattatgtaa aatatcagac agggcccaca
     1981 ctcagaatct gactggggta gagacgcggg acgagaaccg agagcaagaa ctgaaagtga
     2041 aagtgaccac taaagggagg agaggacaga ggggcaggat gtgtcaagat taccagagaa
     2101 cacttggcca gaaatgcgca accattggag ctctccggat tacccaaagg ttaacgagtt
     2161 tgaacgcctc tgcccactcg cccatctctg atggtttccc aagaactcct caagcaaaat
     2221 atatataatt gtgtgtatta tgcacagaca cgagaaaatg ctgtttttct gatctgcatt
     2281 acagcacatt tgcccgccaa cgacaatacc acccactcgg tacctcgctg actcctgatg
     2341 cctgatacct gcgcggtgac tgtctacaat ctgcataatc aagagaagtt gtgttgaaga
     2401 cgagcgccac acaaccgttt ccacaaggtc acccaaggcc ggtgcagatg taggtgaggt
     2461 ctccataaac agactgaaat aaacacatcc tccgctggga acaacaaccc cctcacgcct
     2521 catgcatttc cataagccta catgcatctc ttccaactta tggagactcg cacctaccaa
     2581 catccgcaca acaaagatat acagagcgcg ctccctcagg tcaaggccct gtgggggtct
     2641 gtgcagaaat aggtcatttg tcacacatca agtcctgggg caggagatgc attatagatg
     2701 agaccaaaca gcctgtctcg gtgagctcta cccactccct gagactagaa atgggggaag
     2761 ggagcttgag ataacaaccg ctgcaatcac tgtgtcgatg tttaatatca gcaccaaccg
     2821 ggaacaataa ggagatgggt gcattcatgt tcacatctta ccagtcaagt atcatcgaac
     2881 cggcttgata accacacctc gtgtaatagc tgagcagata gttgtcattt taaagcgttg
     2941 gcctttgtcg attatgtaat gcgcacattc aacacatggt aatatagaaa cggttatgtc
     3001 gaggttgttt tgtccagaga tgaccttcac acagttacag ccgctctgca tccacacaaa
     3061 tggaggactt aatcgtggac tgcattctta gaaatgatct acaaagacaa ataatgtgaa
     3121 atcaagaaag gacaaaattt aagtaagggg atgagggaga gagagaacga ggggcaagga
     3181 gaaagcatgg ctcctgtctt tttctgcacc catctgttcg gagtgcaggt ggagctctat
     3241 tcactcagct ctgcatgtgt gtttgggggg ggcaggaaga aagggagggc aaaaggaaga
     3301 gtggagagat ggtgggggct ggagggatgg ggggttctcg gtgatctctc ctgaagggga
     3361 taatgggaga gcagcgcttt gcaatggctg ccatgtagta ccctccctgc acaattagcc
     3421 aatcagcagc aagctctgcc agccagaagg acacataaaa gaagaacatt gcagcagagg
     3481 cacagaagga gcctgcgagg agctgggaaa tacacacaca acagcagaac cacaacaccc
     3541 tcccctggac acaccctact ggggatcact gcttttcttt ttttctgaac catcgcccac
     3601 gccacacgga gagaaatctc tctctcatca tcatcctgaa gaaaaccccc ttatcctcat
     3661 tttcacactg ctgaggaaaa cctacaatcg cacgggctga gatttcctgg cgaagactgt
     3721 cctttttcct ttttcttttt ttttcttttc ctttggaaac tgacatttgc atttctccat
     3781 tccaagccac ggcgtaataa tatctgcaat ccagcctgaa gacctgcaaa tcgaaggacc
     3841 tagatcacta tcatctttgt acgtcaagga tcctggttac tgtacgtata acctttcttt
     3901 cttttgctct gaccaatatg aaacactaaa atcgattcga gcagcctctc agcatcaatt
     3961 acagtgcgtg aaaaacattc aaatagaggc agcaaatatt acatgtgaaa atacaggctg
     4021 gctaaatcca agctaattta gaaatgtggt caaaacgcat actggcacgt ctaatcgcgg
     4081 attcagtaaa caagattaac gattagccca gtgtataagt catatgatac cggcatgcgc
     4141 gagagcatcg ctacacccga gctggcttca ttttcggagg aaaatcaaaa cattgctttc
     4201 tcctgccgtg cgaaccattc gtcataaacc gtaatacgca acatacattt attactacat
     4261 ccgttaatta gcgataatta gccgttatta acaaagagcg ctgaggaatt cctctcaaat
     4321 agcggaggtg cggcggaggc agaggggcgt aaaagggcac atgcgtgcct ggctcaaaaa
     4381 aggatgctcc agactgaaac tcagagacaa aacacgatgc ctcggaggct gagagcccat
     4441 cgagaggaaa ggcaaagaaa agggtcgagt cactcagagg gggaggggag atattgtgca
     4501 tccattggtt tgaattgaag caggcagaat aaaaatcctt gcgattatgc ttcgtgagag
     4561 atcgcgagag aggaaaaggc taaatgccac gtatgcaaat ggataaatgt catctatttc
     4621 ttcgcgagta gacattttgt ggacaacgag agtgtaaata agcgatatga ggggaataca
     4681 aaggccttga gggaaagcgt tttgggcgtg tcttgtcgaa aagaagacag cgatggcacg
     4741 cgctcagaac caccatcttc acgtagacaa tccgcgtgaa aacaataaca aaactgctca
     4801 ttttaacacg agcaggtgtt tgaaaccacc caaaaaagat tgtctaaaat attaagatta
     4861 attaatgacg tttaaatgtc caggcctgat ataaatgaag cgtgtagtaa gagtaatcgc
     4921 ctttgtttct tttcagagat acgttttcag agattcaggg aattatatct tcaataaaat
     4981 aatgtttgat ttcattattt taaagctttt taagaagtaa aaatacacat tattcattta
     5041 cattttattt tatttaaagg cctatggtct tgaaaataat aagtggaata tataggctat
     5101 atattcgtct aaggacagcc ggtgttattt tatagtgacc tcagacaaaa gccgagacaa
     5161 aacgaggcgc ttgcctttca ttttaatcct taatagacgg tgtgatgaat gaatgaatga
     5221 atgaataaat gaatgaatga cgggcccttc ggcatgttgt tccgcagtcc tccgtgagca
     5281 cgagtctcta ccgcagtgcc aagcacataa aaccgttgag tcagataacc ttctccaggc
     5341 gcgtatacac aattcaagga caagcacatc acaactatac acaacacaac acaaccgcct
     5401 aaacgcacga gctcatcaat cgcaatttaa aggcatgtca gtcaaagaaa aaggcggcgt
     5461 tatttaaaac caatgttcaa caatatttgt atgctgacta gcccgggcgc gcacgctata
     5521 agatcacaag gccttgtttt ttccctttat tcaaaatatc attaattatt acaggtcata
     5581 aaacaaaaca atgcgaatta caatgattta aagactattg atttaacaat aatcagttaa
     5641 ttatagaata atattataat attttacata caaatgaaag atgacattat ttgatgacat
     5701 tgtaattaaa atatatgcaa tatattttta cgtttttatt aattaggcta ttactattat
     5761 tataattatc attattttta ttattattat tattattatt aaatagtctt gcgaaataag
     5821 agtgaaggca gttgcaatgt tcattgttcc cggagagtcc cgcagcctct ggcctcaaca
     5881 caaagcgcta ctgtttacat taatattcat agtgctgcca tggtctctgt agaggaggaa
     5941 cgggtggctc ttttgttcgc tagtgtcaat acgtttattc agcaaaaagc agctcggatt
     6001 tgtgaatgcc caacaagcac acgcgcactc caatcaaagg acgagaggat aacgatttta
     6061 aacgattcgc aaacgtgttt atttgtaaag acgacatcaa gagcttaatg tccacttgaa
     6121 aagaaataaa tatgcccgcg gcgccaagga tattttgaat gtagtcgttc atggtgagcc
     6181 actcccgaat tcagcactct ggagagcgac caaagaggaa tagcggaata gcaactactt
     6241 gagccatttc tctccgtttg atggcgttca tgattaaaaa tataatcacg tgatttttta
     6301 tacatatata aatatataca taaagtaacg ctttcctttt gagaatacat aaatatttat
     6361 tcgcgtgata aatcaatgat cttatattta tttgcacgac cgaaattagc caaattcacc
     6421 aattaaaaaa aaaataagcc aacaaaaaag aagggtttat ccgttcaatt ttgacttgtg
     6481 tcctgttgtt ttacagctgc cactgtgatc ccttaagctg cagtgagagt gtggctaatg
     6541 ccttttgttt aagataatcc tgtaatctgt taccgaaacg gcctattgac aagccggcat
     6601 tcacatttca gtcaggaggc ccgtccagac atgtacattt atgaattacg aatgataaaa
     6661 ttaagatctg cattaggcgt cagaaatgtc acgaacaccc attcatttcc cacagccaat
     6721 aatggatcat gctgggaagc gctatcctcg cagtctcaaa attaatgttg acatgttgcg
     6781 tagagctaca gattagatca gccatatgct ttatgtgttt acctcagcgg atcttcagca
     6841 agatgtgttt attttaagaa aatggcttcc tcgctgcctg cacaggggca ttaaggaagt
     6901 gacgtgagcg tcctcacagt caaattactt tatctcatgc tgctttcagg cctctgctta
     6961 ttttattatt attattttaa attaggggga atcacggtgg cgttgtgggt agggtagcgc
     7021 gatcacctta cagcaagaag gtcgctggtt cgagctctgg ctgggtcagt tggcatttct
     7081 gtgtggagtt taaattgaat tgaaattgaa taaactaaat tggccctagt gtatgtgtgt
     7141 gaatgtaagt gtgcatgggt gtttcccagt gttgggtttg actggaagag catccgcttc
     7201 gtaaaacata tgctggataa gttggcggtt catttcactg tggtgaccgc tgattaataa
     7261 agggactaag ctgaaaatga aatgaattaa tacattttaa atgagctttt gctgacatgt
     7321 ttatgattac aaatagtgaa attgtatgtt aatttacgat aagtgcattg ttctaaattc
     7381 atttttatgt agcagctggt ttttcttcca tttgtgtcat tcaacagaac atttttctaa
     7441 aaaagtgaac ttaaattttc tcctccctct gacaaggctt gatggtaatt tcagccgact
     7501 ctaattacag actcactaaa aggaatcggt gcagtcattt atctttatca gcgcgctggt
     7561 gggaaggtaa taggttttgc ttatgagttg tttgcgtgcg agtctgggtc cttgtgtagg
     7621 cgatgctgat gcactgcatt aaatattgag ggagaagtcg ctcaaaaata gcaaagtaca
     7681 ggctggggca ggaacgtcaa agcccgctca gtggccacgt ttcttgtttc gtaagtccta
     7741 tttttaatag cgataattca ggctcagtag aaggaactcc agcactataa gcagtctctc
     7801 gcctgtctcg gcgagttatg tattttggga tgaagtgctc ttccacctct acctctgctg
     7861 tgctggcgcc agtatctggg ctgtaagata gtgtacgtgt cagtctgtgt ggccgaagag
     7921 cctgtgtgtt gtgtaactca ctacattcgt cgctgtggat ggctgacttg ccatggggag
     7981 gtttacttca gttcacgaat cacattcgcc acgatcttgt cactttgtgt caagactcgc
     8041 tctctgtgtg tcaacagctc tgctgtttga ggttgaaatt ctgaacttga gatatatccc
     8101 aggatatatt agggctactg ccaacggaaa aagcaaatgg tgccatggcc atttgattga
     8161 tcaaacaaac acccttttgc ttgggtgaca gtgaaatgtc agatccatgt ttgtactgct
     8221 ttaataactt gcctctctga gctttctcct tgtcagtgtc aatttgcctg agatataaat
     8281 tccggttgcc ccgaaagctt attttattaa aaaaaagcta acatgcactc atacaacgtg
     8341 cgcacataca tgtgaggctt gctaaaagtc aagaagcaag ccaatagcaa gagtcgtcag
     8401 actgctgtta cctgggcaac ctggttagag gtaaggctca aggcaagaat aaatcattcc
     8461 actgaagaga cacagcgtta cagcgagatg agagcaagag gcgatagtga agggagaaca
     8521 aaagaacagc ctacccacac tagtgcagat ctacctgttt gagctactga ttgttcagtt
     8581 tgattagata tcagacaatt ctgctatctc caattgcatt gctgcatgaa ttcctagaaa
     8641 tcattttaat caccaaaaac tagtagggac acttgtggcg tatatgattc tgcctgttga
     8701 ttgtggggat ccaacagaaa gatcatgaat gcattgttaa ttaagtcggt gagacaccct
     8761 gttccaccct aggggccgtt ctggggaaag agtgctttca gtcagctaaa gatgactaat
     8821 atgtgaacat atattactac acatgccaat ttgtacattc tggatagtta ccagccctgg
     8881 gaaaacatcg aaaaaaataa atcaacatag ataaaattgg caaatccctt ctggtacgat
     8941 gcagagtgct tgctctgaga acttgtgcat tgtgacagaa agcgaccgtt aaaaactgaa
     9001 agtagcatct acaacctgca tacatctgtt ctttgaaaat cccctgtagg ccaaattaag
     9061 catcacgctg tgctccacag caacacggat gaaccgaaaa cccagacaca aacgcacaca
     9121 cttttcacat aaacaaggtt tagttatgct agctatgctt tttcgtcctc cctttgtcat
     9181 cagcggcgag tgacgtaaca cagtttgacc ctggacagcg taaagggggg gttgggaatg
     9241 agtgaaagga ctgtgacatc tgcagggcca ggggctgatg gagagagctg aagtgaggga
     9301 ggtcagagga ggaggaaggg tgggatattg tcctcctcag gccctatccc tctcagacgc
     9361 attgattttt aacctctcag cgagaatgcc aagcattaca cccctcaaat tatggttcct
     9421 cacccaacct gttatatttt ccacctgcag ataattaacc ggtgcaaaca tgctgcagaa
     9481 cgagcttgct cttaagttgg ctggacttga tattaacaag actggaggtt ctcatcatca
     9541 ccaccaccat ggatccatgg cagaaatcgg tactggcttt ccattcgacc cccattatgt
     9601 ggaagtcctg ggcgagcgca tgcactacgt cgatgttggt ccgcgcgatg gcacccctgt
     9661 gctgttcctg cacggtaacc cgacctcctc ctacgtgtgg cgcaacatca tcccgcatgt
     9721 tgcaccgacc catcgctgca ttgctccaga cctgatcggt atgggcaaat ccgacaaacc
     9781 agacctgggt tatttcttcg acgaccacgt ccgcttcatg gatgccttca tcgaagccct
     9841 gggtctggaa gaggtcgtcc tggtcattca cgactggggc tccgctctgg gtttccactg
     9901 ggccaagcgc aatccagagc gcgtcaaagg tattgcattt atggagttca tccgccctat
     9961 cccgacctgg gacgaatggc cagaatttgc ccgcgagacc ttccaggcct tccgcaccac
    10021 cgacgtcggc cgcaagctga tcatcgatca gaacgttttt atcgagtgga cgctgccgat
    10081 ggccgtcgcg cgcgcccgtc gtaaatggca gaaaacaggc catgcggttc gtgctatcgg
    10141 tcgtttgtcg agcggaggct ctggcggttc cggcggttca ggtggatcag atcaattaac
    10201 agaggaacag attgcggagt ttaaggaagc gttctcttta tttgataagg atggcgacgg
    10261 tacaatcact actaaagaat tgggaacagt catgcgctca ttggggcaaa atccgacaga
    10321 ggctgaattg caggacatga ttaacgaggt agacgccgat gggaacggga ctatcgactt
    10381 tccggaattt cttactatga tggcacgcaa aatgaaagat accgattctg aagaagagat
    10441 ccgtgaagcc ttccgtgttt ttgataaaga tgggaacggc tacatcagtg ctgctgagtt
    10501 acgccatgtg atgacaaatc tgggggaaaa acttaccgac gaagaagtag acgaaatgat
    10561 tcgcgaggcg gatattgacg gggatggaca agtaaactac gaggaatttg tgcagatgat
    10621 gaccgccaag tatctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
    10681 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
    10741 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
    10801 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
    10861 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
    10921 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
    10981 cggctaaccg gtggatcctg gccgtcgttt tactgtacca tcgatgatga tccagacatg
    11041 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
    11101 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
    11161 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
    11221 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag
    11281 atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg actgtgctgc
    11341 tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg actgttataa
    11401 tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga catgacgtca
    11461 cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa cgattttcat
    11521 aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat tggtttggta
    11581 atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat
    11641 aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa
    11701 aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt
    11761 acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac cccctacctt
    11821 cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg agctccagct
    11881 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
    11941 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12001 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12061 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12121 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12301 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12961 acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc ttcacctaga
    13021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    13081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    13141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    13201 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13921 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13981 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    14041 taggggttcc gcgcacattt ccccgaaaag tg
//
