LOCUS       sequence-405034-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hSyn1-driven iGluSnFR3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  TITLE     Hart Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FSW_iGluSnFR3.857.GPI
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(66..86)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(484..495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(604..620)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(605..621)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1126..1306
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1332..1349)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1338..1562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1608..2036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1695..1714)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1905..1926
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2045..2065)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2050..2379
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2230..2365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2292..2311
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2427..2474
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2493..2867
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2997..3118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3034..3053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3088..3107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3167..3183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3167..3183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3180..3202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3191..3207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3215..3221,3222..3239,3240..3245))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3260..3281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3398..3415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3569..4157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3649..3668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4328..5119,5120..5188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4951..4970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5189..5293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5368..5387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5559..5938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5940..6138
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6093..6113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6156..6336
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6383..6508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7001..7234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7419..7463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7761..7878
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7944..8391
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      8402..8411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     8408..8467
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             10052..10081
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
       61 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
      121 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
      181 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
      241 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
      301 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
      361 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
      421 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
      481 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
      541 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
      601 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
      661 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
      721 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
      781 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
      841 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
      901 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
      961 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     1021 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     1081 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     1141 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1201 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1261 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     1321 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1381 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1441 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     1501 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     1561 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     1621 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1681 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1741 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1801 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1861 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1921 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1981 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     2041 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2101 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2161 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2221 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2281 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     2341 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     2401 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     2461 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     2521 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     2581 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     2641 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     2701 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     2761 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     2821 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     2881 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     2941 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     3001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3121 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     3181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3241 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     5341 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5401 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     5461 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     5521 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     5581 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     5641 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     5701 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     5761 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     5821 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     5881 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     5941 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     6001 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     6061 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     6121 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     6181 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6241 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6301 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     6361 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6421 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6481 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     6541 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     6601 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     6661 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     6721 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6781 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6841 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     6901 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     6961 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7021 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7081 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7141 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7201 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7261 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7321 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7381 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7441 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7501 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     7561 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     7621 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     7681 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     7741 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7801 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7861 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     7921 aactgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt
     7981 gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc
     8041 caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc
     8101 gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac
     8161 cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt
     8221 cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg
     8281 cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg
     8341 cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gcaaggatcc
     8401 cgccaccatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     8461 cactggtgac agatctgggt ccgccgccgg aagtactctt gataagatcg ctaaaaatgg
     8521 tgtaattgtt gtcggccacc gagactcttc tgtgccattc agctattatg attctcaaca
     8581 caaagtggtg ggctattcac aagagtattc taacgccatt gttgaagcag ttaagaagaa
     8641 gttgaataaa ccagaccttc aagtaaaact cattcctata acatcccaaa accgcatacc
     8701 tctcttgcaa aacggcactt acgatttcga gtgtgggagc actactaata atgtcgaacg
     8761 ccagaaacaa gccgcattct ccgacaccat cttcgtggtg ggcactcgac tgttgacaaa
     8821 gaaaggcggt gatattaagg atttcgcaaa tctgaaggat aaggcagtcg tggtcaccag
     8881 cgggaccacc tcagaagtac tccttaataa actcaatgaa gaacaaaaga tgaatatgcg
     8941 aatcatctct gctaaggacc acggtgactc ttttcgcaca ttggaaagtg ggcgcgccgt
     9001 agcatttatg atggacgact ctctgctcgc tggcgagcgc gccaaagcca aaaaaccaga
     9061 cgactgggaa attgtaggta aacctcaaag ccaagaggca tatggctgca tgttgagaaa
     9121 agatgacccc caattcaaga aactgatgga cgacactata gctcaggtcc agacttcagg
     9181 ggaagctgag aagtggtttg ataaatggtt caagaaccca atcttggtaa atcataccgt
     9241 ttatattacc gctgacaaac aaaagaatgg gataaaggca aatttcaaga ttcaccataa
     9301 cgtagaggat ggaagcgtgc agttggcaga ccattatcaa cagaataccc ctattggcga
     9361 cggacccgtg ctcctccctg acaaccacta cttgtcctat caaagtgttc tctcaaagga
     9421 ccccaatgag aagcgagacc acatggtact tctcgaattc gttaccgcca ccgggaattc
     9481 acttggcatg gatgaattgt ataagggggg tactggcggc agcatgtcca agggtgagga
     9541 gctttttacc ggagtagtac caattctcgt tgaacttgac ggagacgtca acgggcacaa
     9601 gtttagcgta cgaggtgagg gggagggtga tgcaaccaac ggtaaactta ctctcaaatt
     9661 catatgcact accggaaagc tgccagtacc ttggccaaca ctggtcacta ccctctccta
     9721 tggcgttcaa tgttttgctc gataccctga ccacatgaaa cagcatgact tctttaaatc
     9781 agccatgcct gaagggtatg tccaggagcg gactatatca tttaaagacg atggaaccta
     9841 taagactcgg gctgaagtga agctcgaggg tgacacattg gttaatcgga tcgagcttaa
     9901 gggcatagat tttaaagaag atggtaatat tcttggccac aaattggagt acaattttaa
     9961 taatcctttg aacatgaact ttgagctctc cgatgaaatg aaggcactct ttaaagagcc
    10021 aaacgacaaa gctctgaaac tgcaggtcga cgaacaaaaa ctcatctcag aagaggatct
    10081 gtcagataac gactacggta aagatgggtt ggctggggct tcaggaggca gacgaaatgg
    10141 cgctataact gtgctcagtt tcataacgtt ctacgtggca gcatttatgg tattgttgta
    10201 ga
//
