LOCUS       sequence-405041-       10649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse NAIP6 expressed under the constitutive, moderate-level 
            retroviral LTR promoter, expresses in mammalian cells; IRES-GFP 
            reporter downstream of NAIP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-NAIP6
FEATURES             Location/Qualifiers
     source          1..10649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4406..4972
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4422..4974
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4566..4583)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4793..4812
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4986..4988,4989..4991,4992..5705)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5031..5052)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5292..5311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5639..5660
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5767..6281
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6443..6459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6467..6473,6474..6491,6492..6497))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6512..6533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6650..6667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6821..7409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6901..6920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7580..8371,8372..8440))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8203..8222
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8441..8545)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8613..8631
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8669..8691)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8791..8810
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9004..9026
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9154..9173
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             9373..9889
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9953..10294
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(10361..10649,1..128)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatcc gccaccatgg ctgagcatgg ggagtcctcc gaggatcgga
      181 tttctgagat tgattatgaa tttctcgctg agctctctgc tcgtttcggg atgaatctag
      241 ttcagctggc aaagagccaa gaagaagaag accataaaga acggatgaaa atgaagaaag
      301 gttttaactc acagatgcgc agtgaagcca aacgactaaa gacctttgag tcctatgaca
      361 cgttcagatc atggacgcca caggagatgg cagctgctgg gttttaccac acaggggtga
      421 aacttggggt tcagtgcttt tgctgtagct tgatcctctt tggtaacagc ctcaggaagc
      481 ttcccataga gagacacaag aaattacgac cagaatgtga gttccttcag ggaaaagatg
      541 ttggtaacat tggcaagtat gacatccggg tgaagagtcc agagaagatg ctgagaggtg
      601 gcaaagcaag gtaccatgaa gaggaggcca gactggagtc ctttgaggac tggccatttt
      661 atgcccatgg gacatcacca cgtgcactgt cagcagctgg ctttgtcttt acaggtaaaa
      721 gggacactgt gcagtgtttc tcctgtggcg gaagcttggg caactgggaa gaaggagatg
      781 acccctggaa ggagcatgcc aagtggttcc ccaaatgtga atttcttcaa agtaagaaat
      841 cctcagagga aattgcccag tatattcaag actacgaggg atttgttcat gtaacgggag
      901 aacattttgt gaagtcctgg gtcagaagag aattacctat ggtatcagct tactgcaatg
      961 acagtgtctt cactaatgaa gaactaagga tggacatgtt taaggactgg ccccaagaat
     1021 cacctgtggg ttttgaagct ctagtcagag caggcttttt ctacactggc aaaaaggaca
     1081 ttgtccggtg tttttcctgt ggaggatgtt tggagaagtg ggcagaaggt gacgacccaa
     1141 tggaagatca catcaagttt tttcccgaat gtgtatttct tcaaaccttg aagtcctctg
     1201 cagaagtaat tccaaccctt cagagccaat atgcacttcc agaagccacg gaaaccacac
     1261 gtgaaagcaa ccatgatgat gcagcagcag ttcattctac agtggtggac ttgggtagga
     1321 gtgaagctca gtggtttcaa gaggccagga gtctgagtga gcagctgaga gacacctaca
     1381 ctaaaaccag tttctgccac atgaacttgc cagaagtgtg ctccagcctc ggcactgacc
     1441 acttgctcgg ctgtgatgtg tccatcattt caaagcacgt cagccagcct gtgcaagggg
     1501 ccctgacgat cccagaggtc ttctccaatc tcagctctgt catgtgtgtg gagggggaag
     1561 ctggaagtgg aaagacaacc ttcctgaaga ggatagcttt tctctgggca tcaggatgct
     1621 gccccctgtt gtacaggttc cagctggtct tctacctctc ccttagttcc atcacaccag
     1681 atcagggact ggccaacatc atctgtaccc aactcctagg ggcaggaggc tgcattagtg
     1741 aagtgtgtct gagcagcagc atccagcagt tacaacacca ggtgctgttc ctgttggatg
     1801 actacagtgg gctggcctca ctcccccaag ccctacacac actgattaca aaaaactact
     1861 tgttccggac ctgcttattg atcgctgttc atacaaacag ggtcagagac atccgcccat
     1921 acctaggtac aagtctagag atccaagagt ttcccttcta taatactgtc tttgtgttac
     1981 ggaagttttt ttcacatgac ataatctgtg tggaaaagct tataatttac tttagtgaaa
     2041 ataaagactt acagggagtt tacaagaccc ctctcttcgt agcagcagta tgtaatgact
     2101 ggaatcaaaa tgcatctgcc caggatgact ttcaagatgt gacacttttc cattcctaca
     2161 tgcaatacct gtccttaaaa tacaaagcta cagctgagtc tctccaggcc actgtgtcct
     2221 catgtgggca gctggccttg acagggcttt tctcatcgtg ctttgagttc aatagtgatg
     2281 acctggcaga ggcaggagtt gatgaagatg taaagcttac caccttcttg atgagcaaat
     2341 tcaccgccca gagactgagg ccagtctacc gatttttagg tcctctgttc caggagtttc
     2401 ttgctgccgt gaggttgact gaactcctga gttcagatag gcaggaagac caagatcttg
     2461 gactttatta tttgagacaa attgactcac ctctgaaggc aataaactcc tttaacatat
     2521 ttttgtatta tgtctccagc cactcttcct caaaggcagc gccaacagtt gtatctcatt
     2581 tgcttcagtt ggtggatgag aaagagtcac tggagaacat gtctgaaaat gaggattaca
     2641 tgaagctcca tccacaaact tttctatggt ttcagtttgt tagagggttg tggctggtgt
     2701 ctcctgaatc tttctcttca ttcgtttcag aacatttatt gcgccttgct ctaatctttg
     2761 cttatgaaag caacacagtt gctgaatgct ctccatttat tttgcaattc cttcgaggaa
     2821 gaacactggc tttaagagta ctgaatttag agtacttttg ggaccaccca gaaagcctgt
     2881 tactgttgag gagtttaaag gtttccataa atggaaataa aatgtcatct tatgtagatt
     2941 attcatttaa gacatatttt gaaaacttac agccaccagc tataaatgag gagtatacat
     3001 ctgcctttga gcatgtaagt gaatggagga gaaattttgc tcaagatgag gagatcataa
     3061 aaaactatga aaatatctgg cccagagccc taccagacat cagtgaaggg tactggaatc
     3121 tgtcccccaa gccatgcaag atccccaagc tggaagttca agtgaacaac atgggtccag
     3181 cagaccaagc actgctccag gtcctcatgg aagtcttctc agcttcacag agtattgagt
     3241 tccatttatt caacagcagt ggcttccttg aaagcatccg cccagctctg gagctgagta
     3301 aggcctctgt caccaagtgt tccatgtcca ggctggagct cagcagagca gaacaggagt
     3361 tgcttctcac cctgcctgcc ctgcagtctc tcgaggtctc agagacaaac cagttaccag
     3421 atcagctctt ccataacttg cacaagttcc tgggcctgaa agaactgtgt gtgagactag
     3481 atggcaaacc ggatgtgctc tcagtccttc ctgaagagtt cctgaacctc catcacatgg
     3541 agaagttatc catcagaacc tctacagagt ctgacctctc caaactagtt aaattcatcc
     3601 agaactttcc aaatctccat gttttccatc tgaaatgtga tttcctttca aattgtgagt
     3661 ctctcatgac tgcgcttgct tcctgcaaga aactcagaga gattgagttt tctggacaat
     3721 gctttgaagc catgaccttt gtcaacattt tgccaaattt tgtttctctg aagatactga
     3781 gtcttaaagg tcaacaattt gcagataagg aaacatcaga aaagtttgcc caggctctgg
     3841 gttctctcag gaacttagag gaactgcttg ttcccactgg ggatgggatt caccaagtgg
     3901 ccaaactgat tgtccggcag tgtctgcagc ttccgtgcct ccgagttctc gcctttcacg
     3961 acatcttgga cgatgaaagt gtgattgaaa ttgccagggc ggcaaccagt ggaagtttcc
     4021 agaaacttga aaacttagat atttcaatga atcacaagat taccgaggaa ggatacagaa
     4081 atttctttca agccctggac aacttgccaa acctacaaat gctgaacatc tgcaggaata
     4141 tcccaggacg cattcaagtt caggccacca ctgtcaaggc tttgggtcac tgtgtgtccc
     4201 gactgcccag cctcaccagg ctggggatgc tcagttggct cctggatgaa gaggacatga
     4261 aagtgattaa tgatgtgaag gaaagacacc cccagtccaa acgcttgact atcttctgga
     4321 aatggatagt cccgttctct cctgttgtcc tggagtaagc ggccgcgttt aaacgtcgac
     4381 ggtatcgata agcttgatat cgaattccgc ccccccccct aacgttactg gccgaagccg
     4441 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4501 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4561 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4621 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4681 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     4741 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     4801 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4861 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     4921 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     4981 caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     5041 tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca
     5101 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     5161 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     5221 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     5281 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5341 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5401 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     5461 agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     5521 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     5581 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     5641 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     5701 agtaatgaat taattaagaa ttcgataaaa taaaagattt tatttagtct ccagaaaaag
     5761 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     5821 aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga
     5881 gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     5941 gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca
     6001 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     6061 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     6121 acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtgta
     6181 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     6241 tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag tttcttgaag
     6301 ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac tcaattagcc
     6361 actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga cagcgcagaa
     6421 agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6481 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6541 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6601 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6661 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6721 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6781 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6901 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6961 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7021 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7081 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7141 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7201 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7261 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7321 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7381 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7441 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7501 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7561 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7621 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7681 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7741 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7801 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7861 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7921 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7981 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8041 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8101 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8161 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8221 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8281 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8341 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8401 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8461 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8521 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8581 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8641 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8701 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8761 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8821 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     8881 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8941 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9001 tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc
     9061 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     9121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     9181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt
     9241 ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca
     9301 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca
     9361 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     9421 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
     9481 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     9541 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
     9601 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     9661 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     9721 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac
     9781 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg
     9841 ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcattt ggaggttcca
     9901 ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag gtaagctggc
     9961 cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt
    10021 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
    10081 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg
    10141 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt
    10201 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
    10261 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg
    10321 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
    10381 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
    10441 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
    10501 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
    10561 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
    10621 cttggctttt gacccccctc cctgggtca
//
