LOCUS       sequence-405069-        1753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Elu ES1 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo14
FEATURES             Location/Qualifiers
     source          1..1753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(31..133)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     oriT            293..402
                     /note="incP origin of transfer"
     rep_origin      517..905
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      1076..1103
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1124..1753,1..30))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     1698..1717
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
       61 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      121 tacgtgccga tcagtcaatt cgagggggat caattccgtt ttccgctgca taaccctgct
      181 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
      241 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
      301 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
      361 tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag
      421 atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca
      481 cggaattgat ccggccacga tgcgtccggc gtagaggatc tgaagatcag cagttcaacc
      541 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
      601 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
      661 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
      721 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
      781 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
      841 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc
      901 tgacatgggg gtctcggaga agacgttatc aattgccgca ttatagccta ttaacgaaga
      961 cctttcatgg cgtcggacaa tcaggtacta tcgaagggct cattacaatt tattaacata
     1021 agatcagtaa caaccagaag cgctcgaagg agaggttatt acgagacctc acactggctc
     1081 accttcgggt gggcctttct gcgtttatac ctaggagtca aaattactcc ccgccctgcc
     1141 actcatcgca gtactgttgt aattcattaa gcattctacc gacatggaag ccatcacaaa
     1201 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1261 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1321 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     1381 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1441 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1501 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1561 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1621 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     1681 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     1741 gggatatatc aac
//
