LOCUS       sequence-405080-        1881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Elu ES3 insert with a chloramphenicol
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo16
FEATURES             Location/Qualifiers
     source          1..1881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      23..50
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(71..730)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     645..664
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(731..833)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     oriT            993..1102
                     /note="incP origin of transfer"
     rep_origin      1217..1605
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 taaaattacg agacctcaca ctggctcacc ttcgggtggg cctttctgcg tttataccta
       61 ggagtcaaaa ttactccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
      121 ttctaccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
      181 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
      241 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
      301 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
      361 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
      421 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
      481 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
      541 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
      601 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
      661 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
      721 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
      781 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcagtcaatt
      841 cgagggggat caattccgtt ttccgctgca taaccctgct tcggggtcat tatagcgatt
      901 ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag ggttcgtgta
      961 gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt aggcccaccc
     1021 gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct caacgggaat
     1081 cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag atgagggcaa gcggatggct
     1141 gatgaaacca agccaaccag gaagggcagc ccacctatca cggaattgat ccggccacga
     1201 tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     1261 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     1321 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
     1381 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
     1441 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta
     1501 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc
     1561 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatgggg gtctcggaga
     1621 cgtggtatcg gcgacaaggt tgaaatccgt gaatgccgtc cactgtccaa gactaagtcc
     1681 tggacgctgg ttcgcgttgt agagaaagcg gttctgtaat agagtacgca ttctcaatac
     1741 gaataaacgg ctcagcgatg agccgtttat tttttctacc catattgtag aagcggtgtt
     1801 ataatgccgc gccctcgata tggggctttt taacggctct gattttagag tctcaggtag
     1861 tagttgacat tagcggagca c
//
