LOCUS       sequence-405085-        1861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the Lambda tl3 terminator insert with a 
            chloramphenicol resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo11
FEATURES             Location/Qualifiers
     source          1..1861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      24..412
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(428..672)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     terminator      691..718
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(739..1398)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGE"
     primer_bind     1313..1332
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1399..1501)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     oriT            1661..1770
                     /note="incP origin of transfer"
ORIGIN
        1 gccacgatgc gtccggtgta gaggatctga agatcagcag ttcaacctgt tgatagtacg
       61 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
      121 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
      181 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
      241 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
      301 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
      361 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga catgggggtc
      421 tcgggtacta ctggtattgg cacaaacctg attccaattt gagcaaggct atgtgccatc
      481 tcgatactcg ttcttaactc aacagaagat gctttgtgca tacagcccct cgtttattat
      541 ttatctcctc agccagccgc tgtgctttca gtggatttcg gataacagaa aggccgggaa
      601 atacccagcc tcgctttgta acggagtaga cgaaagtgat tgcgcctacc cggatattat
      661 cgtgaggatg cgctcccgag acctcacact ggctcacctt cgggtgggcc tttctgcgtt
      721 tatacctagg agtcaaaatt actccccgcc ctgccactca tcgcagtact gttgtaattc
      781 attaagcatt ctaccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      841 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
      901 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
      961 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     1021 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     1081 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     1141 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat
     1201 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1261 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1321 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1381 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1441 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     1501 agtcaattcg agggggatca attccgtttt ccgctgcata accctgcttc ggggtcatta
     1561 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1621 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     1681 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     1741 acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc
     1801 ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcacg gaattgatcc
     1861 g
//
