LOCUS       sequence-405091-       13125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid allows for inducible expression of engineered 
            BRD4-p300(HI) fusion tagged with mNeonGreen, in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Kosno M, Currie SL, Kumar A, Xing C, Rosen MK
  TITLE     Molecular features driving condensate formation and gene expression 
            by the BRD4-NUT fusion oncoprotein are overlapping but distinct.
  JOURNAL   Sci Rep. 2023 Jul 24;13(1):11907. doi: 10.1038/s41598-023-39102-9.
  PUBMED    37488172
REFERENCE   2  (bases 1 to 13125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-07-24"; volume: "13(1):11907. doi"; pages: " 
            10.1038/s41598-023-39102-9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(HI)
FEATURES             Location/Qualifiers
     source          1..13125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1016
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1020..1724
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      6987..6996
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9077..9094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9083..9307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9440..9459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9591..9638
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9646..10668
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9646..10668
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10798..10919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10835..10854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10889..10908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10968..10984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10968..10984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10981..11003)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10992..11008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11016..11022,11023..11040,11041..11046))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11061..11082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11199..11216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11370..11958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11450..11469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12129..12920,12921..12989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12752..12771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12990..13094)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatgggac cggctgccaa gcgcgtgaaa ctcgatggcg
     1021 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
     1081 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
     1141 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
     1201 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
     1261 cgcctttcca ggccgcaatg gtagatggct ccggatacca agtccatcgc acaatgcagt
     1321 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
     1381 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
     1441 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
     1501 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
     1561 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
     1621 acgtgttccg taagacggaa ctcaagcact ccaagaccga actcaacttc aaggagtggc
     1681 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caagggcggc tcgtacgtgt
     1741 acaagtctgc ggagagcggc cctgggacga gattgagaaa tctgccagta atgggggatg
     1801 gactagaaac ttcccaaatg tctacaacac aggcccaggc ccaaccccag ccagccaacg
     1861 cagccagcac caaccccccg cccccagaga cctccaaccc taacaagccc aagaggcaga
     1921 ccaaccaact gcaatacctg ctcagagtgg tgctcaagac actatggaaa caccagtttg
     1981 catggccttt ccagcagcct gtggatgccg tcaagctgaa cctccctgat tactataaga
     2041 tcattaaaac gcctatggat atgggaacaa taaagaagcg cttggaaaac aactattact
     2101 ggaatgctca ggaatgtatc caggacttca acactatgtt tacaaattgt tacatctaca
     2161 acaagcctgg agatgacata gtcttaatgg cagaagctct ggaaaagctc ttcttgcaaa
     2221 aaataaatga gctacccaca gaagaaaccg agatcatgat agtccaggca aaaggaagag
     2281 gacgtgggag gaaagaaaca gggacagcaa aacctggcgt ttccacggta ccaaacacaa
     2341 ctcaagcatc gactcctccg cagacccaga cccctcagcc gaatcctcct cctgtgcagg
     2401 ccacgcctca ccccttccct gccgtcaccc cggacctcat cgtccagacc cctgtcatga
     2461 cagtggtgcc tccccagcca ctgcagacgc ccccgccagt gcccccccag ccacaacccc
     2521 cacccgctcc agctccccag cccgtacaga gccacccacc catcatcgcg gccaccccac
     2581 agcctgtgaa gacaaagaag ggagtgaaga ggaaagcaga caccaccacc cccaccacca
     2641 ttgaccccat tcacgagcca ccctcgctgc ccccggagcc caagaccacc aagctgggcc
     2701 agcggcggga gagcagccgg cctgtgaaac ctccaaagaa ggacgtgccc gactctcagc
     2761 agcacccagc accagagaag agcagcaagg tctcggagca gctcaagtgc tgcagcggca
     2821 tcctcaagga gatgtttgcc aagaagcacg ccgcctacgc ctggcccttc tacaagcctg
     2881 tggacgtgga ggcactgggc ctacacgact actgtgacat catcaagcac cccatggaca
     2941 tgagcacaat caagtctaaa ctggaggccc gtgagtaccg tgatgctcag gagtttggtg
     3001 ctgacgtccg attgatgttc tccaactgct ataagtacaa ccctcctgac catgaggtgg
     3061 tggccatggc ccgcaagctc caggatgtgt tcgaaatgcg ctttgccaag atgccggacg
     3121 agcctgagga gccagtggtg gccgtgtcct ccccggcagt gccccctccc accaaggttg
     3181 tggccccgcc ctcatccagc gacagcagca gcgatagctc ctcggacagt gacagttcga
     3241 ctgatgactc tgaggaggag cgagcccagc ggctggctga gctccaggag cagctcaaag
     3301 ccgtgcacga gcagcttgca gccctctctc agccccagca gaacaaacca aagaaaaagg
     3361 agaaagacaa gaaggaaaag aaaaaagaaa agcacaaaag gaaagaggaa gtggaagaga
     3421 ataaaaaaag caaagccaag gaacctcctc ctaaaaagac gaagaaaaat aatagcagca
     3481 acagcaatgt gagcaagaag gagccagcgc ccatgaagag caagccccct cccacgtatg
     3541 agtcggagga agaggacaag tgcaagccta tgtcctatga ggagaagcgg cagctcagct
     3601 tggacatcaa caagctcccc ggcgagaagc tgggccgcgt ggtgcacatc atccagtcac
     3661 gggagccctc cctgaagaat tccaaccccg acgagattga aatcgacttt gagaccctga
     3721 agccgtccac actgcgtgag ctggagcgct atgtcacctc ctgtttgcgg aagaaaagga
     3781 aacctcaagc tgagaaagtt gatgtgattg ccggctcctc caagatgaag ggcttctcgt
     3841 cctcagagtc ggagagctcc agtgagtcca gctcctctga cagcgaagac tccgaaacaa
     3901 tggccgagaa tgtggtggaa ccggggccgc cttcagccaa gcggcctaaa ctctcatctc
     3961 cggccctctc ggcgtccgcc agcgatggca cagattttgg ctctctattt gacttggagc
     4021 acgacttacc agatgaatta atcaactcta cagaattggg actaaccaat ggtggtgata
     4081 ttaatcagct tcagacaagt cttggcatgg tacaagatgc agcttctaaa cataaacagc
     4141 tgtcagaatt gctgcgatct ggtagttccc ctaacctcaa tatgggagtt ggtggcccag
     4201 gtcaagtcat ggccagccag gcccaacaga gcagtcctgg attaggtttg ataaatagca
     4261 tggtcaaaag cccaatgaca caggcaggct tgacttctcc caacatgggg atgggcacta
     4321 gtggaccaaa tcagggtcct acgcagtcaa caggtatgat gaacagtcca gtaaatcagc
     4381 ctgccatggg aatgaacaca gggatgaatg cgggcatgaa tcctggaatg ttggctgcag
     4441 gcaatggaca agggataatg cctaatcaag tcatgaacgg ttcaattgga gcaggccgag
     4501 ggcgacagaa tatgcagtac ccaaacccag gcatgggaag tgctggcaac ttactgactg
     4561 agcctcttca gcagggctct ccccagatgg gaggacaaac aggattgaga ggcccccagc
     4621 ctcttaagat gggaatgatg aacaacccca atccttatgg ttcaccatat actcagaatc
     4681 ctggacagca gattggagcc agtggccttg gtctccagat tcagacaaaa actgtactat
     4741 caaataactt atctccattt gctatggaca aaaaggcagt tcctggtgga ggaatgccca
     4801 acatgggtca acagccagcc ccgcaggtcc agcagccagg tctggtgact ccagttgccc
     4861 aagggatggg ttctggagca catacagctg aaaaacgaag gaccagacta cagaagcaga
     4921 acatgctacc aaatgctgca ggcatggttc cagtttccat gaatccaggg cctaacatgg
     4981 gacagccgca accaggaatg acttctaatg gccctctacc tgacccaagt atgatccgtg
     5041 gcagtgtgcc aaaccagatg atgcctcgaa taactccaca atctggtttg aatcaatttg
     5101 gccagatgag catggcccag ccccctattg taccccggca aacccctcct cttcagcacc
     5161 atggacagtt ggctcaacct ggagctctca acccgcctat gggctatggg cctcgtatgc
     5221 aacagccttc caaccagggc cagttccttc ctcagactca gttcccatca cagggaatga
     5281 atgtaacaaa tatccctttg gctccgtcca gcggtcaagc tccagtgtct caagcacaaa
     5341 tgtctagttc ttcctgcccg gtgaactctc ctataatgcc tccagggtct caggggagcc
     5401 acattcactg tccccagctt cctcaaccag ctcttcatca gaattcaccc tcgcctgtac
     5461 ctagtcgtac ccccacccct caccatactc ccccaagcat aggggctcag cagccaccag
     5521 caacaacaat tccagcccct gttcctacac ctcctgccat gccacctggg ccacagtccc
     5581 aggctctaca tccccctcca aggcagacac ctacaccacc aacaacacaa cttccccaac
     5641 aagtgcagcc ttcacttcct gctgcacctt ctgctgacca gccccagcag cagcctcgct
     5701 cacagcagag cacagcagcg tctgttccta ccccaacagc accgctgctt cctccgcagc
     5761 ctgcaactcc actttcccag ccagctgtaa gcattgaagg acaggtatca aatcctccat
     5821 ctactagtag cacagaagtg aattctcagg ccattgctga gaagcagcct tcccaggaag
     5881 tgaagatgga ggccaaaatg gaagtggatc aaccagaacc agcagatact cagccggagg
     5941 atatttcaga gtctaaagtg gaagactgta aaatggaatc taccgaaaca gaagagagaa
     6001 gcactgagtt aaaaactgaa ataaaagagg aggaagacca gccaagtact tcagctaccc
     6061 agtcatctcc ggctccagga cagtcaaaga aaaagtctgc taaaaggttg ccatctacca
     6121 gacttggcac ctttctagag aatcgtgtga atgactttct gaggcgacag aatcaccctg
     6181 agtcaggaga ggtcactgtt agagtagttc atgcttctga caaaaccgtg gaagtaaaac
     6241 caggcatgaa agcaaggttt gtggacagtg gagagatggc agaatccttt ccataccgaa
     6301 ccaaagccct ctttgccttt gaagaaattg atggtgttga cctgtgcttc tttggcatgc
     6361 atgttcaaga gtatggctct gactgccctc cacccaacca gaggagagta tacatatctt
     6421 acctcgatag tgttcatttc ttccgtccta aatgcttgag gactgcagtc tatcatgaaa
     6481 tcctaattgg atatttagaa tatgtcaaga aattaggtta cacaacaggg catatttggg
     6541 catgtccacc aagtgaggga gatgattata tcttccattg ccatcctcct gaccagaaga
     6601 tacccaagcc caagcgactg caggaatggt acaaaaaaat gcttgacaag gctgtatcag
     6661 agcgtattgt ccatgactac aaggatattt ttaaacaagc tactgaagat agattaacaa
     6721 gtgcaaagga attgccttat ttcgagggtg atttctggcc caatgttctg gaagaaagca
     6781 ttaaggaact ggaacaggag gaagaagaga gaaaacgaga ggaaaacacc agcaatgaaa
     6841 gcacagatgt gaccaaggga gacagcaaaa atgctaaaaa gaagaataat aagaaaacca
     6901 gcaaaaataa gagcagcctg agtaggggca acaagaagaa acccgggatg cccaatgtat
     6961 ctaacgacct ctcacagaaa ctatatgcca ccatggagaa gcataaagag gtcttctttg
     7021 tgatccgcct cattgctggc cctgctgcca actccctgcc tcccattgtt gatcctgatc
     7081 ctctcatccc ctgcgatctg atggatggtc gggatgcgtt tctcacgctg gcaagggaca
     7141 agcacctgga gttctcttca ctccgaagag cccagtggtc caccatgtgc atgctggtgg
     7201 agctgcacac gcagagccag cagaagctcc ggcagcaaca gctgcagcac cgactacagc
     7261 aggcccaaat gcttcgcagg aggatggcca gcatgcagcg gactggtgtg gttgggcagc
     7321 aacagggcct cccttccccc actcctgcca ctccaacgac accaactggc caacagccaa
     7381 ccaccccgca gacgccccag cccacttctc agcctcagcc tacccctccc aatagcatgc
     7441 caccctactt gcccaggact caagctgctg gccctgtgtc ccagggtaag gcagcaggcc
     7501 aggtgacccc tccaacccct cctcagactg ctcagccacc ccttccaggg cccccacctg
     7561 cagcagtgga aatggcaatg cagattcaga gagcagcgga gacgcagcgc cagatggccc
     7621 acgtgcaaat ttttcaaagg ccaatccaac accagatgcc cccgatgact cccatggccc
     7681 ccatgggtat gaacccacct cccatgacca gaggtcccag tgggcatttg gagccaggga
     7741 tgggaccgac agggatgcag caacagccac cctggagcca aggaggattg cctcagcccc
     7801 agcaactaca gtctgggatg ccaaggccag ccatgatgtc agtggcccag catggtcaac
     7861 ctttgaacat ggctccacaa ccaggattgg gccaggtagg tatcagccca ctcaaaccag
     7921 gcactgtgtc tcaacaagcc ttacaaaacc ttttgcggac tctcaggtct cccagctctc
     7981 ccctgcagca gcaacaggtg cttagtatcc ttcacgccaa cccccagctg ttggctgcat
     8041 tcatcaagca gcgggctgcc aagtatgcca actctaatcc acaacccatc cctgggcagc
     8101 ctggcatgcc ccaggggcag ccagggctac agccacctac catgccaggt cagcaggggg
     8161 tccactccaa tccagccatg cagaacatga atccaatgca ggcgggcgtt cagagggctg
     8221 gcctgcccca gcagcaacca cagcagcaac tccagccacc catgggaggg atgagccccc
     8281 aggctcagca gatgaacatg aaccacaaca ccatgccttc acaattccga gacatcttga
     8341 gacgacagca aatgatgcaa cagcagcagc aacagggagc agggccagga ataggccctg
     8401 gaatggccaa ccataaccag ttccagcaac cccaaggagt tggctaccca ccacagcagc
     8461 agcagcggat gcagcatcac atgcaacaga tgcaacaagg aaatatggga cagataggcc
     8521 agcttcccca ggccttggga gcagaggcag gtgccagtct acaggcctat cagcagcgac
     8581 tccttcagca acagatgggg tcccctgttc agcccaaccc catgagcccc cagcagcata
     8641 tgctcccaaa tcaggcccag tccccacacc tacaaggcca gcagatccct aattctctct
     8701 ccaatcaagt gcgctctccc cagcctgtcc cttctccacg gccacagtcc cagccccccc
     8761 actccagtcc ttccccaagg atgcagcctc agccttctcc acaccacgtt tccccacaga
     8821 caagttcccc acatcctgga ctggtagctg cccaggccaa ccccatggaa caagggcatt
     8881 ttgccagccc ggaccagaat tcaatgcttt ctcagcttgc tagcaatcca ggcatggcaa
     8941 acctccatgg tgcaagcgcc acggacctgg gactcagcac cgataactca gacttgaatt
     9001 caaacctctc acagagtaca ctagacatat gagcggccgc tcgagtctag agggcccgtt
     9061 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9121 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9181 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9241 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9301 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     9361 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9421 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9481 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     9541 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta
     9601 ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc
     9661 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     9721 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     9781 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     9841 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     9901 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     9961 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
    10021 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
    10081 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
    10141 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
    10201 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
    10261 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
    10321 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
    10381 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
    10441 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
    10501 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
    10561 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
    10621 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga
    10681 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    10741 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
    10801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10921 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
    10981 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    11041 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    11101 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    11161 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    11221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    11281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    11341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    11581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    11821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12001 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    12061 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    12121 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    12181 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    12241 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    12301 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    12361 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    12421 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    12481 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    12541 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    12601 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    12661 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    12721 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    12781 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    12841 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    12901 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    12961 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    13021 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    13081 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
