LOCUS       sequence-405093-        1615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid containing the chloramphenicol resistance gene 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   van Schaik J, Li Z, Cheadle J, Crook N
  TITLE     Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and 
            Identification of Potential Bacterial Chassis for Studying 
            Plant-Microbe Interactions.
  JOURNAL   ACS Synth Biol. 2023 Oct 20;12(10):3030-3040. doi: 
            10.1021/acssynbio.3c00371. Epub 2023 Sep 15.
  PUBMED    37712562
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00371"; journalName: "ACS Synth Biol"; date: 
            "2023-10-20- 20"; volume: "12"; issue: "10"; pages: "3030-3040"
COMMENT     Sequence Label: pMRMTK-clo5
FEATURES             Location/Qualifiers
     source          1..1615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            176..285
                     /note="incP origin of transfer"
     promoter        456..558
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             559..1218
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(625..644)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      complement(1239..1266)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      join(1289..1615,1..62)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgct gatcttcaga
       61 tcctctacgc cggacgcatc gtggccggat caattccgtg ataggtggct gcccttcctg
      121 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
      181 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
      241 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
      301 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
      361 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaac
      421 ggaattgatc cccctcgaat tgacggtctc gcgcttgatc ggcacgtaag aggttccaac
      481 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
      541 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      601 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      661 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      721 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
      781 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
      841 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
      901 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
      961 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     1021 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1081 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1141 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1201 tggcagggcg gggcgtaatt ttgactccta ggtataaacg cagaaaggcc cacccgaagg
     1261 tgagccagtg tgaggagcga gacccccatg tcagccgtta agtgttcctg tgtcactcaa
     1321 aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct tgttgtccac
     1381 aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct tataaaactt
     1441 aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc aaacatgaga
     1501 gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag tacgttagcc
     1561 atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga gctta
//
